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- W3004718043 abstract "Abstract The synovium secretes synovial fluid, but is also richly innervated with nociceptors and acts as a gateway between avascular joint tissues and the circulatory system. Resident fibroblast-like synoviocytes’ (FLS) calcium-activated potassium channels (K Ca ) change in activity in arthritis models and this correlates with FLS activation . Objective To investigate this activation in an in vitro model of inflammatory arthritis; 72hr treatment with cytokines TNFα and IL1β. Methods FLS cells were isolated from rat synovial membranes. We analysed global changes in FLS mRNA by RNA-sequencing and then focused on FLS ion channels genes and corresponding FLS electrophysiological phenotype, finally modelling data with Ingenuity Pathway Analysis (IPA) and MATLAB. Results IPA showed significant activation of inflammatory, osteoarthritic and calcium signalling canonical pathways by cytokines, and we identified ~200 channel gene transcripts. The large K Ca (BK) channel consists of the pore forming Kcnma1 together with β-subunits. Following cytokine treatment, a significant increase in Kcnma1 RNA abundance was detected by qPCR and changes in several ion channels were detected by RNA-sequencing, including a loss of BK channel β-subunit expression Kcnmb1/2 and increase in Kcnmb3. In electrophysiological experiments, there was a decrease in overall current density at 20mV without change in chord conductance at this potential. Conclusion TNFα and IL1β treatment of FLS in vitro recapitulated several common features of inflammatory arthritis at the transcriptomic level, including increase in Kcnma1 and Kcnmb3 gene expression." @default.
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- W3004718043 date "2020-02-07" @default.
- W3004718043 modified "2023-09-24" @default.
- W3004718043 title "Pro-inflammatory cytokines drive deregulation of potassium channel expression in primary synovial fibroblasts" @default.
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- W3004718043 doi "https://doi.org/10.1101/2020.02.06.937078" @default.
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