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• W3004896317 abstract "We developed a multiplex real-time SYBR green-based PCR assay for rapid detection of tet (X) and its variants, including tet (X1) and tet (X2) and high-level tigecycline resistance genes tet (X3), tet (X4), and tet (X5). We showed that the real-time PCR assay developed had high linearity ( R 2 ≥ 0.996), sensitivity (low detection limit), and specificity (only the target gene could be amplified significantly) and further evaluated it using bacterial, fecal, and environmental samples." @default.
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• W3004896317 date "2020-03-24" @default.
• W3004896317 modified "2023-10-16" @default.
• W3004896317 title "Development of a Multiplex Real-Time PCR Assay for Rapid Detection of Tigecycline Resistance Gene <i>tet</i> (X) Variants from Bacterial, Fecal, and Environmental Samples" @default.
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• W3004896317 doi "https://doi.org/10.1128/aac.02292-19" @default.
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