Matches in SemOpenAlex for { <https://semopenalex.org/work/W3005102949> ?p ?o ?g. }
- W3005102949 endingPage "279" @default.
- W3005102949 startingPage "264" @default.
- W3005102949 abstract "Viral protein 9 (VP9) is a non-structural protein of white spot syndrome virus (WSSV) highly expressed during the early stage of infection. The crystal structure of VP9 suggests that the polymers of VP9 dimers resemble a DNA mimic, but its function remains elusive. In this study, we demonstrated that VP9 impedes histones binding to DNA via single-molecule manipulation. We established VP9 expression in HeLa cells due to the lack of a WSSV-susceptible cell line, and observed abundant VP9 in the nucleus, which mirrors its distribution in the hemocytes of WSSV-infected shrimp. VP9 expression increased the dynamics and rotational mobility of histones in stable H3-GFP HeLa cells as revealed by fluorescent recovery after photobleaching and fluorescence anisotropy imaging, which suggested a loosened compaction of chromatin structure. Successive salt fractionation showed that a prominent population of histones was solubilized in high salt concentrations, which implies alterations of bulk chromatin structure. Southern blotting identified that VP9 alters juxtacentromeric chromatin structures to be more accessible to micrococcal nuclease digestion. RNA microarray revealed that VP9 expression also leads to significant changes of cellular gene expression. Our findings provide evidence that VP9 alters the cellular higher-order chromatin structure, uncovering a potential strategy adopted by WSSV to facilitate its replication." @default.
- W3005102949 created "2020-02-14" @default.
- W3005102949 creator A5009749498 @default.
- W3005102949 creator A5015314071 @default.
- W3005102949 creator A5025350443 @default.
- W3005102949 creator A5043992281 @default.
- W3005102949 creator A5044374570 @default.
- W3005102949 date "2020-04-01" @default.
- W3005102949 modified "2023-10-13" @default.
- W3005102949 title "White spot syndrome viral protein VP9 alters the cellular higher‐order chromatin structure" @default.
- W3005102949 cites W135309739 @default.
- W3005102949 cites W1514653354 @default.
- W3005102949 cites W152585140 @default.
- W3005102949 cites W1547045190 @default.
- W3005102949 cites W1558882143 @default.
- W3005102949 cites W1584471230 @default.
- W3005102949 cites W1590920410 @default.
- W3005102949 cites W1603627149 @default.
- W3005102949 cites W1608797052 @default.
- W3005102949 cites W1899455654 @default.
- W3005102949 cites W1980042844 @default.
- W3005102949 cites W1980224338 @default.
- W3005102949 cites W1984870657 @default.
- W3005102949 cites W1986605851 @default.
- W3005102949 cites W1987333937 @default.
- W3005102949 cites W1993434836 @default.
- W3005102949 cites W1995782808 @default.
- W3005102949 cites W2000531240 @default.
- W3005102949 cites W2000537175 @default.
- W3005102949 cites W2001165481 @default.
- W3005102949 cites W2002698073 @default.
- W3005102949 cites W2002831389 @default.
- W3005102949 cites W2003435166 @default.
- W3005102949 cites W2003835295 @default.
- W3005102949 cites W2005502890 @default.
- W3005102949 cites W2006528064 @default.
- W3005102949 cites W2012042594 @default.
- W3005102949 cites W2014278482 @default.
- W3005102949 cites W2014461994 @default.
- W3005102949 cites W2019314918 @default.
- W3005102949 cites W2021387021 @default.
- W3005102949 cites W2023365382 @default.
- W3005102949 cites W2025947376 @default.
- W3005102949 cites W2031224669 @default.
- W3005102949 cites W2037837681 @default.
- W3005102949 cites W2043388821 @default.
- W3005102949 cites W2043929240 @default.
- W3005102949 cites W2048217707 @default.
- W3005102949 cites W2051127506 @default.
- W3005102949 cites W2060073273 @default.
- W3005102949 cites W2062320783 @default.
- W3005102949 cites W2062832688 @default.
- W3005102949 cites W2063104329 @default.
- W3005102949 cites W2063994725 @default.
- W3005102949 cites W2072869314 @default.
- W3005102949 cites W2077177939 @default.
- W3005102949 cites W2080646845 @default.
- W3005102949 cites W2081764012 @default.
- W3005102949 cites W2084280180 @default.
- W3005102949 cites W2088626432 @default.
- W3005102949 cites W2091404580 @default.
- W3005102949 cites W2097803426 @default.
- W3005102949 cites W2098103685 @default.
- W3005102949 cites W2106863384 @default.
- W3005102949 cites W2113221891 @default.
- W3005102949 cites W2115369674 @default.
- W3005102949 cites W2119362410 @default.
- W3005102949 cites W2121353617 @default.
- W3005102949 cites W2129098490 @default.
- W3005102949 cites W2140388293 @default.
- W3005102949 cites W2143351999 @default.
- W3005102949 cites W2143599753 @default.
- W3005102949 cites W2144800232 @default.
- W3005102949 cites W2147524392 @default.
- W3005102949 cites W2147600078 @default.
- W3005102949 cites W2151040185 @default.
- W3005102949 cites W2156384317 @default.
- W3005102949 cites W2158217645 @default.
- W3005102949 cites W2159210918 @default.
- W3005102949 cites W2159634882 @default.
- W3005102949 cites W2172400873 @default.
- W3005102949 cites W2174957569 @default.
- W3005102949 cites W2175556166 @default.
- W3005102949 cites W4117022 @default.
- W3005102949 cites W4250003364 @default.
- W3005102949 doi "https://doi.org/10.1096/fba.2019-00086" @default.
- W3005102949 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7133739" @default.
- W3005102949 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32259052" @default.
- W3005102949 hasPublicationYear "2020" @default.
- W3005102949 type Work @default.
- W3005102949 sameAs 3005102949 @default.
- W3005102949 citedByCount "1" @default.
- W3005102949 countsByYear W30051029492022 @default.
- W3005102949 crossrefType "journal-article" @default.
- W3005102949 hasAuthorship W3005102949A5009749498 @default.
- W3005102949 hasAuthorship W3005102949A5015314071 @default.
- W3005102949 hasAuthorship W3005102949A5025350443 @default.
- W3005102949 hasAuthorship W3005102949A5043992281 @default.