Matches in SemOpenAlex for { <https://semopenalex.org/work/W3005138049> ?p ?o ?g. }
- W3005138049 abstract "ABSTRACT Spatial transcriptomics seeks to integrate single-cell transcriptomic data within the 3-dimensional space of multicellular biology. Current methods use glass substrates pre-seeded with matrices of barcodes or fluorescence hybridization of a limited number of probes. We developed an alternative approach, called ‘ZipSeq’, that uses patterned illumination and photocaged oligonucleotides to serially print barcodes (Zipcodes) onto live cells within intact tissues, in real-time and with on-the-fly selection of patterns. Using ZipSeq, we mapped gene expression in three settings: in-vitro wound healing, live lymph node sections and in a live tumor microenvironment (TME). In all cases, we discovered new gene expression patterns associated with histological structures. In the TME, this demonstrated a trajectory of myeloid and T cell differentiation, from periphery inward. A variation of ZipSeq efficiently scales to the level of single cells, providing a pathway for complete mapping of live tissues, subsequent to real-time imaging or perturbation." @default.
- W3005138049 created "2020-02-14" @default.
- W3005138049 creator A5013943962 @default.
- W3005138049 creator A5016217664 @default.
- W3005138049 creator A5018179968 @default.
- W3005138049 creator A5025368177 @default.
- W3005138049 creator A5030109235 @default.
- W3005138049 creator A5049526512 @default.
- W3005138049 creator A5052760522 @default.
- W3005138049 creator A5078302316 @default.
- W3005138049 creator A5090253536 @default.
- W3005138049 creator A5091824858 @default.
- W3005138049 date "2020-02-04" @default.
- W3005138049 modified "2023-09-24" @default.
- W3005138049 title "ZipSeq : Barcoding for Real-time Mapping of Single Cell Transcriptomes" @default.
- W3005138049 cites W1664076455 @default.
- W3005138049 cites W1969057487 @default.
- W3005138049 cites W1984883254 @default.
- W3005138049 cites W1985314055 @default.
- W3005138049 cites W1988391018 @default.
- W3005138049 cites W2008603652 @default.
- W3005138049 cites W2029531805 @default.
- W3005138049 cites W2043565262 @default.
- W3005138049 cites W2047564123 @default.
- W3005138049 cites W2049472439 @default.
- W3005138049 cites W2068767807 @default.
- W3005138049 cites W2071117491 @default.
- W3005138049 cites W2099698731 @default.
- W3005138049 cites W2102212449 @default.
- W3005138049 cites W2113231837 @default.
- W3005138049 cites W2121235217 @default.
- W3005138049 cites W2148356659 @default.
- W3005138049 cites W2159803625 @default.
- W3005138049 cites W2162068902 @default.
- W3005138049 cites W2170921356 @default.
- W3005138049 cites W2175332918 @default.
- W3005138049 cites W2184331293 @default.
- W3005138049 cites W2471536144 @default.
- W3005138049 cites W2506616866 @default.
- W3005138049 cites W2528538594 @default.
- W3005138049 cites W2592935721 @default.
- W3005138049 cites W2593476638 @default.
- W3005138049 cites W2739492614 @default.
- W3005138049 cites W2756389915 @default.
- W3005138049 cites W2771767991 @default.
- W3005138049 cites W2793411374 @default.
- W3005138049 cites W2890107533 @default.
- W3005138049 cites W2901513648 @default.
- W3005138049 cites W2911559653 @default.
- W3005138049 cites W2915848182 @default.
- W3005138049 cites W2947391351 @default.
- W3005138049 cites W2949238013 @default.
- W3005138049 cites W2951068818 @default.
- W3005138049 cites W2951506174 @default.
- W3005138049 cites W2961304458 @default.
- W3005138049 cites W2972983657 @default.
- W3005138049 cites W2973055525 @default.
- W3005138049 doi "https://doi.org/10.1101/2020.02.04.932988" @default.
- W3005138049 hasPublicationYear "2020" @default.
- W3005138049 type Work @default.
- W3005138049 sameAs 3005138049 @default.
- W3005138049 citedByCount "4" @default.
- W3005138049 countsByYear W30051380492020 @default.
- W3005138049 countsByYear W30051380492021 @default.
- W3005138049 countsByYear W30051380492022 @default.
- W3005138049 crossrefType "posted-content" @default.
- W3005138049 hasAuthorship W3005138049A5013943962 @default.
- W3005138049 hasAuthorship W3005138049A5016217664 @default.
- W3005138049 hasAuthorship W3005138049A5018179968 @default.
- W3005138049 hasAuthorship W3005138049A5025368177 @default.
- W3005138049 hasAuthorship W3005138049A5030109235 @default.
- W3005138049 hasAuthorship W3005138049A5049526512 @default.
- W3005138049 hasAuthorship W3005138049A5052760522 @default.
- W3005138049 hasAuthorship W3005138049A5078302316 @default.
- W3005138049 hasAuthorship W3005138049A5090253536 @default.
- W3005138049 hasAuthorship W3005138049A5091824858 @default.
- W3005138049 hasBestOaLocation W30051380491 @default.
- W3005138049 hasConcept C104317684 @default.
- W3005138049 hasConcept C1491633281 @default.
- W3005138049 hasConcept C150194340 @default.
- W3005138049 hasConcept C162317418 @default.
- W3005138049 hasConcept C54355233 @default.
- W3005138049 hasConcept C70721500 @default.
- W3005138049 hasConcept C74318829 @default.
- W3005138049 hasConcept C86803240 @default.
- W3005138049 hasConcept C95444343 @default.
- W3005138049 hasConceptScore W3005138049C104317684 @default.
- W3005138049 hasConceptScore W3005138049C1491633281 @default.
- W3005138049 hasConceptScore W3005138049C150194340 @default.
- W3005138049 hasConceptScore W3005138049C162317418 @default.
- W3005138049 hasConceptScore W3005138049C54355233 @default.
- W3005138049 hasConceptScore W3005138049C70721500 @default.
- W3005138049 hasConceptScore W3005138049C74318829 @default.
- W3005138049 hasConceptScore W3005138049C86803240 @default.
- W3005138049 hasConceptScore W3005138049C95444343 @default.
- W3005138049 hasLocation W30051380491 @default.
- W3005138049 hasOpenAccess W3005138049 @default.
- W3005138049 hasPrimaryLocation W30051380491 @default.
- W3005138049 hasRelatedWork W11392542 @default.
- W3005138049 hasRelatedWork W11596768 @default.