Matches in SemOpenAlex for { <https://semopenalex.org/work/W3005144411> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W3005144411 abstract "High specificity of trypsin is a prerequisite for accurate identification and quantification of proteins in shotgun proteomics. It is important to minimize nonspecific enzymatic cleavages during proteomic sample preparation. Methods In this study, protein extraction and trypsin digestion conditions were extensively evaluated using the less-complex Escherichia coli lysates to improve the sensitivity of detecting low-abundance nonspecific peptides by liquid chromatography/tandem mass spectrometry. Results Trypsin digestion buffers and digestion times were proved to have a significant effect on nonspecific cleavages. The triethylammonium bicarbonate buffer induces significantly lower nonspecific cleavages than the other two buffers, but a freshly prepared urea solution does not induce more than sodium dodecyl sulfate. Because prolonged trypsin digestion resulted in a considerable number of nonspecific cleavages, an optimized 2-h protocol was developed with 45.2% less semispecific tryptic peptides but 18.5% more unmodified peptides identified than the commonly used 16-h protocol. Conclusions The significant decrease in nonspecific cleavages and artificial modifications improves the accuracy of protein quantification and the identification of low-abundance proteins, and it is especially useful for studying protein posttranslational modifications. For trypsin digestion, the proposed 2-h protocol can potentially be a replacement for the traditional 16-h protocol." @default.
- W3005144411 created "2020-02-14" @default.
- W3005144411 creator A5004969934 @default.
- W3005144411 creator A5011244338 @default.
- W3005144411 creator A5022175957 @default.
- W3005144411 creator A5045898861 @default.
- W3005144411 creator A5063569282 @default.
- W3005144411 creator A5064765042 @default.
- W3005144411 creator A5068460027 @default.
- W3005144411 date "2020-03-05" @default.
- W3005144411 modified "2023-10-18" @default.
- W3005144411 title "Evaluation and minimization of nonspecific tryptic cleavages in proteomic sample preparation" @default.
- W3005144411 cites W1877838458 @default.
- W3005144411 cites W1970133096 @default.
- W3005144411 cites W1984336508 @default.
- W3005144411 cites W1996593009 @default.
- W3005144411 cites W2023096047 @default.
- W3005144411 cites W2036321220 @default.
- W3005144411 cites W2048386842 @default.
- W3005144411 cites W2053943711 @default.
- W3005144411 cites W2065986307 @default.
- W3005144411 cites W2080752012 @default.
- W3005144411 cites W2096057003 @default.
- W3005144411 cites W2097594688 @default.
- W3005144411 cites W2097942944 @default.
- W3005144411 cites W2115511703 @default.
- W3005144411 cites W2125499226 @default.
- W3005144411 cites W2129450490 @default.
- W3005144411 cites W2138460371 @default.
- W3005144411 cites W2139525501 @default.
- W3005144411 cites W2141036148 @default.
- W3005144411 cites W2160074540 @default.
- W3005144411 cites W2164522178 @default.
- W3005144411 cites W2168270559 @default.
- W3005144411 cites W2298659676 @default.
- W3005144411 cites W2299289681 @default.
- W3005144411 cites W2312465510 @default.
- W3005144411 cites W2321436468 @default.
- W3005144411 cites W2325020798 @default.
- W3005144411 cites W2892286792 @default.
- W3005144411 cites W2910730299 @default.
- W3005144411 cites W2923671190 @default.
- W3005144411 cites W2792412764 @default.
- W3005144411 doi "https://doi.org/10.1002/rcm.8733" @default.
- W3005144411 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32031715" @default.
- W3005144411 hasPublicationYear "2020" @default.
- W3005144411 type Work @default.
- W3005144411 sameAs 3005144411 @default.
- W3005144411 citedByCount "8" @default.
- W3005144411 countsByYear W30051444112020 @default.
- W3005144411 countsByYear W30051444112021 @default.
- W3005144411 countsByYear W30051444112022 @default.
- W3005144411 countsByYear W30051444112023 @default.
- W3005144411 crossrefType "journal-article" @default.
- W3005144411 hasAuthorship W3005144411A5004969934 @default.
- W3005144411 hasAuthorship W3005144411A5011244338 @default.
- W3005144411 hasAuthorship W3005144411A5022175957 @default.
- W3005144411 hasAuthorship W3005144411A5045898861 @default.
- W3005144411 hasAuthorship W3005144411A5063569282 @default.
- W3005144411 hasAuthorship W3005144411A5064765042 @default.
- W3005144411 hasAuthorship W3005144411A5068460027 @default.
- W3005144411 hasConcept C185592680 @default.
- W3005144411 hasConcept C198531522 @default.
- W3005144411 hasConcept C2778576202 @default.
- W3005144411 hasConcept C43617362 @default.
- W3005144411 hasConceptScore W3005144411C185592680 @default.
- W3005144411 hasConceptScore W3005144411C198531522 @default.
- W3005144411 hasConceptScore W3005144411C2778576202 @default.
- W3005144411 hasConceptScore W3005144411C43617362 @default.
- W3005144411 hasFunder F4320327675 @default.
- W3005144411 hasIssue "10" @default.
- W3005144411 hasLocation W30051444111 @default.
- W3005144411 hasOpenAccess W3005144411 @default.
- W3005144411 hasPrimaryLocation W30051444111 @default.
- W3005144411 hasRelatedWork W1518870844 @default.
- W3005144411 hasRelatedWork W1964667958 @default.
- W3005144411 hasRelatedWork W2005102210 @default.
- W3005144411 hasRelatedWork W2032357048 @default.
- W3005144411 hasRelatedWork W2349445879 @default.
- W3005144411 hasRelatedWork W2966172367 @default.
- W3005144411 hasRelatedWork W3087918462 @default.
- W3005144411 hasRelatedWork W4238238172 @default.
- W3005144411 hasRelatedWork W10798041 @default.
- W3005144411 hasRelatedWork W2059332397 @default.
- W3005144411 hasVolume "34" @default.
- W3005144411 isParatext "false" @default.
- W3005144411 isRetracted "false" @default.
- W3005144411 magId "3005144411" @default.
- W3005144411 workType "article" @default.