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- W3005192095 abstract "Molecular tools that reliably recognize tumors, cells, and their components are essential to cancer research, diagnosis, and treatment. However, due to the shortcomings of current reagent generation methods (e.g., hybridoma technology, phage display, yeast display), these reagents are often poorly characterized and extremely expensive. Thus, there is a pressing need for inexpensive ways to generate high-quality target-directed reagents. mRNA display is a powerful molecular selection technology that identifies ultrahigh affinity reagents to cancer-relevant targets among libraries of more than a trillion unique molecules. To enrich the peptides with high affinity to the target, a library of peptides is bound to the immobilized target. Subsequently, the unbound peptides are washed off and the remaining peptides are amplified. For high selectivity, it is important to minimize rebinding of the unbound peptides during washing. The current method to minimize rebinding requires adding excess free target after an initial wash, so that an unbound peptide binds to a free target instead of an immobilized target. However, this technique is costly because of the amount of target consumed. In this study, the selection step of mRNA display was performed in a microfluidic device where the target was immobilized and the unbound peptides were washed away by a continuous flow. Using this technique, the enrichment of high affinity peptides to cancer marker Bcl-xL was demonstrated. By reducing the amount of target needed during the selection step, continuous-flow microfluidics enables mRNA display to be performed economically and efficiently, making it broadly accessible to the research community." @default.
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- W3005192095 date "2020-02-01" @default.
- W3005192095 modified "2023-10-16" @default.
- W3005192095 title "Preparation of Peptides with High Affinity to Cancer Targets in MRNA Display via Continuous-Flow Microfluidics" @default.
- W3005192095 doi "https://doi.org/10.1016/j.bpj.2019.11.950" @default.
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