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- W3005267238 abstract "Single-molecule force spectroscopy techniques are powerful tools for investigating the mechanical unfolding of biomolecules. However, they are limited in throughput and low temporal resolution, restricting the utility of these techniques and the theoretical interpretation of current experiments. Motivated by these limitations, here, we report a polymer force clamp (PFC) that can unfold target molecules on-demand. The PFC consists of a plasmonic nanorod core encapsulated within a thermo-responsive polymer particle. Optical heating of the nanorod leads to the rapid collapse of the polymer, thus transducing light into mechanical work to unfold target molecules. The polymer particle functions as an excellent experimental tool to harness the input photon energy to deliver piconewton (pN) mechanical force on conjugated biological systems (i.e. biomolecules) with high spatiotemporal resolution. Most importantly, the particle collapse is highly tunable in space, time, and force magnitude, which happens to be an essential requirement of mechanobiological studies, as the delivery of a precise amount of mechanical force without thermal denaturation or alteration of the native properties of the biological sample is needed. Besides, single-molecule fluorescence imaging showed reproducible mechanical unfolding of DNA hairpins with the polymer particles. Last but not least, combining PFC with DNA technology, one can spectroscopically acquire time-resolved mechanical unfolding dynamics of biomolecules, and to efficiently boost the throughput of the force manipulation on biomolecules." @default.
- W3005267238 created "2020-02-14" @default.
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- W3005267238 date "2020-02-01" @default.
- W3005267238 modified "2023-09-27" @default.
- W3005267238 title "Polymer Force Clamps for the Mechanical Unfolding of Target Molecules" @default.
- W3005267238 doi "https://doi.org/10.1016/j.bpj.2019.11.2014" @default.
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