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- W3005403222 abstract "Bacterial mechanosensitive channels gate in response to tension in the membrane driven by dramatic shifts in osmolarity, often rescuing the cell from cell death. In Escherichia coli the mechanosensitive channel of small conductance (MscS) and the mechanosensitive channel of large conductance (MscL) gate in response to mechanical tension across the membrane. E. coli MscS is the most well studied member of the MscS superfamily of ion channels, a genetically diverse group of channels that often contain additional domains on either the N and C termini when compared to MscS. In many bacterial genomes several MscS members are predicted. This duplicity in bacterial genomes is relatively rare and suggests that MscS superfamily members mayhave multiple essential roles within the cell cycle. E. coli MscL is the model channel for the MscL family of channels, which typically contain two transmembrane domains and a large non-selective pore. Paraburkholderia graminis, a soil bacterium responsible for carbon sequestration and nitrogen fixation, is predicted to have six unique MscS superfamily members and a single MscL channel. These predicted MscS superfamily members fall into three subfamilies: bacterial cyclic nucleotide gated, multi-transmembrane, and an extended N-terminus family. To study these channels in their native environment, we developed a protocol to create giant spheroplasts for use in patch clamp electrophysiology. When mechanical tension is applied to native Paraburkholderia graminis membranes three unique conductances are observed: a mini, a small, and a large conductance. The pressure threshold ratio between the small and large conductance channels is comparable to the pressure threshold ratio observed in E. coli. The mini conductance channel opens at similar pressure to the large conductance channel. The presence of active mechanosensitive channels in native P. graminis membranes shows that soil bacterial contain similar mechanisms to E. coli to survive osmotic changes." @default.
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- W3005403222 date "2020-02-01" @default.
- W3005403222 modified "2023-09-26" @default.
- W3005403222 title "Mechanosensitive Channels in Paraburkholderia Graminis" @default.
- W3005403222 doi "https://doi.org/10.1016/j.bpj.2019.11.254" @default.
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