FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3005487997> ?p ?o ?g. }

• W3005487997 abstract "Abstract Long non-coding (lnc) RNAs represent a fascinating class of transcripts that remains highly controversial mainly due to ambiguity surrounding overall biological relevance of these RNAs. Multitude of reverse genetics studies showing functionality of lncRNAs are unfortunately based on assays that are either plagued by non-specific effects and/or cannot unambiguously assign observed phenotypes to the transcript per se . Here, we show application of the novel CRISPR/Cas13 RNA knockdown system that has superior specificity compared to other transcript-targeting knockdown methods like RNAi. We applied this method to a novel widespread subclass of nuclear lncRNAs — very long intergenic non-coding (vlinc) RNAs — in a high-throughput phenotypic assay based on survival challenge in response to anticancer drug treatments. We used multiple layers of controls including mismatch control for each targeting gRNA to ensure uncovering true phenotype-transcript relationships. We found evidence supporting importance for cellular survival for up to 60% of the tested protein-coding mRNAs and, importantly, 64% of vlincRNAs. Overall, this study demonstrates utility of CRISPR/Cas13 as a highly sensitive and specific tool for reverse genetics study of both protein-coding genes and lncRNAs. Furthermore, importantly, this approach provides evidence supporting biological significance of the latter transcripts in anticancer drug response." @default.
• W3005487997 created "2020-02-14" @default.
• W3005487997 creator A5005245031 @default.
• W3005487997 creator A5008738173 @default.
• W3005487997 creator A5014572828 @default.
• W3005487997 creator A5020457439 @default.
• W3005487997 creator A5038732762 @default.
• W3005487997 creator A5065865566 @default.
• W3005487997 creator A5086903709 @default.
• W3005487997 creator A5088905218 @default.
• W3005487997 date "2020-02-04" @default.
• W3005487997 modified "2023-10-15" @default.
• W3005487997 title "A CRISPR/Cas13-based approach demonstrates biological relevance of vlinc class of long non-coding RNAs in anticancer drug response" @default.
• W3005487997 cites W1934704664 @default.
• W3005487997 cites W1975288407 @default.
• W3005487997 cites W2000629769 @default.
• W3005487997 cites W2002297333 @default.
• W3005487997 cites W2007870233 @default.
• W3005487997 cites W2016086822 @default.
• W3005487997 cites W2016927884 @default.
• W3005487997 cites W2027954222 @default.
• W3005487997 cites W2034547555 @default.
• W3005487997 cites W2038138598 @default.
• W3005487997 cites W2051907464 @default.
• W3005487997 cites W2058615001 @default.
• W3005487997 cites W2081383642 @default.
• W3005487997 cites W2083042132 @default.
• W3005487997 cites W2091338613 @default.
• W3005487997 cites W2099468911 @default.
• W3005487997 cites W2105142153 @default.
• W3005487997 cites W2105349784 @default.
• W3005487997 cites W2106948237 @default.
• W3005487997 cites W2109699478 @default.
• W3005487997 cites W2119838510 @default.
• W3005487997 cites W2120077570 @default.
• W3005487997 cites W2128016314 @default.
• W3005487997 cites W2128552624 @default.
• W3005487997 cites W2136963255 @default.
• W3005487997 cites W2143393352 @default.
• W3005487997 cites W2153175562 @default.
• W3005487997 cites W2154169171 @default.
• W3005487997 cites W2166810745 @default.
• W3005487997 cites W2179438025 @default.
• W3005487997 cites W2239874339 @default.
• W3005487997 cites W2259938310 @default.
• W3005487997 cites W2311751391 @default.
• W3005487997 cites W2325972250 @default.
• W3005487997 cites W2468606088 @default.
• W3005487997 cites W2514110482 @default.
• W3005487997 cites W2566949887 @default.
• W3005487997 cites W2592811885 @default.
• W3005487997 cites W2763042654 @default.
• W3005487997 cites W2770685832 @default.
• W3005487997 cites W2783011989 @default.
• W3005487997 cites W2793392982 @default.
• W3005487997 cites W2884685868 @default.
• W3005487997 cites W2900899200 @default.
• W3005487997 cites W2909742725 @default.
• W3005487997 cites W2968526019 @default.
• W3005487997 cites W4250359879 @default.
• W3005487997 doi "https://doi.org/10.1038/s41598-020-58104-5" @default.
• W3005487997 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7000768" @default.
• W3005487997 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32020014" @default.
• W3005487997 hasPublicationYear "2020" @default.
• W3005487997 type Work @default.
• W3005487997 sameAs 3005487997 @default.
• W3005487997 citedByCount "42" @default.
• W3005487997 countsByYear W30054879972020 @default.
• W3005487997 countsByYear W30054879972021 @default.
• W3005487997 countsByYear W30054879972022 @default.
• W3005487997 countsByYear W30054879972023 @default.
• W3005487997 crossrefType "journal-article" @default.
• W3005487997 hasAuthorship W3005487997A5005245031 @default.
• W3005487997 hasAuthorship W3005487997A5008738173 @default.
• W3005487997 hasAuthorship W3005487997A5014572828 @default.
• W3005487997 hasAuthorship W3005487997A5020457439 @default.
• W3005487997 hasAuthorship W3005487997A5038732762 @default.
• W3005487997 hasAuthorship W3005487997A5065865566 @default.
• W3005487997 hasAuthorship W3005487997A5086903709 @default.
• W3005487997 hasAuthorship W3005487997A5088905218 @default.
• W3005487997 hasBestOaLocation W30054879971 @default.
• W3005487997 hasConcept C104317684 @default.
• W3005487997 hasConcept C127716648 @default.
• W3005487997 hasConcept C166703698 @default.
• W3005487997 hasConcept C173396325 @default.
• W3005487997 hasConcept C194993378 @default.
• W3005487997 hasConcept C54355233 @default.
• W3005487997 hasConcept C62203573 @default.
• W3005487997 hasConcept C67705224 @default.
• W3005487997 hasConcept C70721500 @default.
• W3005487997 hasConcept C86803240 @default.
• W3005487997 hasConcept C98108389 @default.
• W3005487997 hasConceptScore W3005487997C104317684 @default.
• W3005487997 hasConceptScore W3005487997C127716648 @default.
• W3005487997 hasConceptScore W3005487997C166703698 @default.
• W3005487997 hasConceptScore W3005487997C173396325 @default.
• W3005487997 hasConceptScore W3005487997C194993378 @default.
• W3005487997 hasConceptScore W3005487997C54355233 @default.
• W3005487997 hasConceptScore W3005487997C62203573 @default.
• W3005487997 hasConceptScore W3005487997C67705224 @default.

• next