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• W3005545701 abstract "Abstract The main objective of the study was to develop a bioprocess to hydrolyse galacto-oligosaccharides (GOs) using genetically engineered Pseudomonas chlororaphis which could produce biopolymers and biosurfactants. To this end, alpha-galactosidase genes were expressed in P. chlororaphis transformants either via a plasmid vector (i.e., strain [dAG]) or integrated into the chromosome (i.e., strain [chr::AG]). Pseudomonas secretion signals for protein were tested to facilitate extracellular secretion of the gene product. Furthermore, a two-stage condensed-cell-density fermentation process was adopted to enable hydrolysis of GOs by the P. chlororaphis recombinants. The results showed that the recombinant P. chlororaphis strains could hydrolyse GOs in culture media containing raffinose alone, stachyose alone, crude soy molasses, and tofu whey waste byproducts. Specifically, the most active recombinant P. chlororaphis [dAG] strain reduced the levels of raffinose (0.5% w/v) to" @default.
• W3005545701 created "2020-02-24" @default.
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• W3005545701 date "2020-03-01" @default.
• W3005545701 modified "2023-09-27" @default.
• W3005545701 title "Bioprocess for hydrolysis of galacto-oligosaccharides in soy molasses and tofu whey by recombinant Pseudomonas chlororaphis" @default.
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• W3005545701 doi "https://doi.org/10.1016/j.bcab.2020.101529" @default.
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