Matches in SemOpenAlex for { <https://semopenalex.org/work/W3005655146> ?p ?o ?g. }
- W3005655146 endingPage "1160" @default.
- W3005655146 startingPage "1136" @default.
- W3005655146 abstract "Unlike C3 plants, Crassulacean acid metabolism (CAM) plants fix CO2 in the dark using phosphoenolpyruvate carboxylase (PPC; EC 4.1.1.31). PPC combines phosphoenolpyruvate with CO2 (as HCO3-), forming oxaloacetate. The oxaloacetate is converted to malate, leading to malic acid accumulation in the vacuole, which peaks at dawn. During the light period, malate decarboxylation concentrates CO2 around Rubisco for secondary fixation. CAM mutants lacking PPC have not been described. Here, we employed RNA interference to silence the CAM isogene PPC1 in Kalanchoë laxiflora Line rPPC1-B lacked PPC1 transcripts, PPC activity, dark period CO2 fixation, and nocturnal malate accumulation. Light period stomatal closure was also perturbed, and the plants displayed reduced but detectable dark period stomatal conductance and arrhythmia of the CAM CO2 fixation circadian rhythm under constant light and temperature free-running conditions. By contrast, the rhythm of delayed fluorescence was enhanced in plants lacking PPC1 Furthermore, a subset of gene transcripts within the central circadian oscillator was upregulated and oscillated robustly in this line. The regulation of guard cell genes involved in controlling stomatal movements was also perturbed in rPPC1-B These findings provide direct evidence that the regulatory patterns of key guard cell signaling genes are linked with the characteristic inverse pattern of stomatal opening and closing during CAM." @default.
- W3005655146 created "2020-02-24" @default.
- W3005655146 creator A5003224959 @default.
- W3005655146 creator A5047686097 @default.
- W3005655146 creator A5058762072 @default.
- W3005655146 creator A5062764928 @default.
- W3005655146 creator A5075718407 @default.
- W3005655146 creator A5078809383 @default.
- W3005655146 creator A5084605117 @default.
- W3005655146 date "2020-02-12" @default.
- W3005655146 modified "2023-10-15" @default.
- W3005655146 title "<i>Kalanchoë</i> PPC1 Is Essential for Crassulacean Acid Metabolism and the Regulation of Core Circadian Clock and Guard Cell Signaling Genes" @default.
- W3005655146 cites W1484075860 @default.
- W3005655146 cites W1513693018 @default.
- W3005655146 cites W1853231671 @default.
- W3005655146 cites W1901022830 @default.
- W3005655146 cites W1920667152 @default.
- W3005655146 cites W1930867597 @default.
- W3005655146 cites W1968209592 @default.
- W3005655146 cites W1973743529 @default.
- W3005655146 cites W1974429249 @default.
- W3005655146 cites W1974907033 @default.
- W3005655146 cites W1975361738 @default.
- W3005655146 cites W1975996842 @default.
- W3005655146 cites W1980111781 @default.
- W3005655146 cites W1981529935 @default.
- W3005655146 cites W1983289412 @default.
- W3005655146 cites W1984639268 @default.
- W3005655146 cites W1990528941 @default.
- W3005655146 cites W1990707565 @default.
- W3005655146 cites W1997038372 @default.
- W3005655146 cites W1999931859 @default.
- W3005655146 cites W2007482320 @default.
- W3005655146 cites W2011007693 @default.
- W3005655146 cites W2011104448 @default.
- W3005655146 cites W2017168486 @default.
- W3005655146 cites W2019702445 @default.
- W3005655146 cites W2024064642 @default.
- W3005655146 cites W2027374968 @default.
- W3005655146 cites W2036131768 @default.
- W3005655146 cites W2042167665 @default.
- W3005655146 cites W2047123786 @default.
- W3005655146 cites W2048117041 @default.
- W3005655146 cites W2052370382 @default.
- W3005655146 cites W2058651702 @default.
- W3005655146 cites W2060687391 @default.
- W3005655146 cites W2062027605 @default.
- W3005655146 cites W2075860357 @default.
- W3005655146 cites W2076043250 @default.
- W3005655146 cites W2077074817 @default.
- W3005655146 cites W2077545872 @default.
- W3005655146 cites W2078929441 @default.
- W3005655146 cites W2081629276 @default.
- W3005655146 cites W2081977049 @default.
- W3005655146 cites W2083479533 @default.
- W3005655146 cites W2089238244 @default.
- W3005655146 cites W2093250314 @default.
- W3005655146 cites W2097404112 @default.
- W3005655146 cites W2108900585 @default.
- W3005655146 cites W2108906483 @default.
- W3005655146 cites W2113298484 @default.
- W3005655146 cites W2113523707 @default.
- W3005655146 cites W2114773774 @default.
- W3005655146 cites W2121017657 @default.
- W3005655146 cites W2121631499 @default.
- W3005655146 cites W2122207098 @default.
- W3005655146 cites W2124956928 @default.
- W3005655146 cites W2125136905 @default.
- W3005655146 cites W2127849304 @default.
- W3005655146 cites W2131205184 @default.
- W3005655146 cites W2133528607 @default.
- W3005655146 cites W2136727913 @default.
- W3005655146 cites W2137069308 @default.
- W3005655146 cites W2152231376 @default.
- W3005655146 cites W2154173720 @default.
- W3005655146 cites W2156513800 @default.
- W3005655146 cites W2156723264 @default.
- W3005655146 cites W2159476659 @default.
- W3005655146 cites W2169966075 @default.
- W3005655146 cites W2171878353 @default.
- W3005655146 cites W2173163031 @default.
- W3005655146 cites W2178552215 @default.
- W3005655146 cites W2188385721 @default.
- W3005655146 cites W2222440211 @default.
- W3005655146 cites W2265398774 @default.
- W3005655146 cites W2340232012 @default.
- W3005655146 cites W2340716304 @default.
- W3005655146 cites W2340984222 @default.
- W3005655146 cites W2507204728 @default.
- W3005655146 cites W2534816540 @default.
- W3005655146 cites W2591873856 @default.
- W3005655146 cites W2596314872 @default.
- W3005655146 cites W2606996598 @default.
- W3005655146 cites W273553601 @default.
- W3005655146 cites W2737398968 @default.
- W3005655146 cites W2748763306 @default.
- W3005655146 cites W2752020941 @default.
- W3005655146 cites W2753475659 @default.