Matches in SemOpenAlex for { <https://semopenalex.org/work/W3005822432> ?p ?o ?g. }
- W3005822432 abstract "Abstract Actinobacillus pleuropneumoniae is an important veterinary pathogen that causes porcine pleuropneumonia. Lipoproteins of bacterial pathogens play pleiotropic roles in the infection process. In addition, many bacterial lipoproteins are antigenic and immunoprotective. Therefore, characterization of lipoproteins is a promising strategy for identification of novel vaccine candidates or diagnostic markers. We cloned 58 lipoproteins from A . pleuropneumoniae JL03 (serovar 3) and expressed them in Escherichia coli . Five proteins with strong positive signals in western blotting analysis were used to immunize mice. These proteins elicited significant antibody responses, and three of them (APJL_0922, APJL_1380 and APJL_1976) generated efficient immunoprotection in mice against lethal heterologous challenge with A . pleuropneumoniae 4074 (serovar 1), both in the active and passive immunization assays. Then immunogenicity of these three lipoproteins (APJL_0922, APJL_1380 and APJL_1976) were further tested in pigs. Results showed that these proteins elicited considerable humoral immune responses and effective protective immunity against virulent A . pleuropneumoniae challenge. Our findings suggest that these three novel lipoproteins could be potential subunit vaccine candidates." @default.
- W3005822432 created "2020-02-24" @default.
- W3005822432 creator A5014889588 @default.
- W3005822432 creator A5016677423 @default.
- W3005822432 creator A5019391870 @default.
- W3005822432 creator A5026869217 @default.
- W3005822432 creator A5028652883 @default.
- W3005822432 creator A5038112478 @default.
- W3005822432 creator A5044702979 @default.
- W3005822432 creator A5050470383 @default.
- W3005822432 creator A5066716873 @default.
- W3005822432 date "2020-02-11" @default.
- W3005822432 modified "2023-09-24" @default.
- W3005822432 title "Genome-wide screening of lipoproteins in Actinobacillus pleuropneumoniae identifies three antigens that confer protection against virulent challenge" @default.
- W3005822432 cites W114568797 @default.
- W3005822432 cites W1969851729 @default.
- W3005822432 cites W1973875183 @default.
- W3005822432 cites W1981273940 @default.
- W3005822432 cites W1981776522 @default.
- W3005822432 cites W1996223704 @default.
- W3005822432 cites W1999530100 @default.
- W3005822432 cites W2013358742 @default.
- W3005822432 cites W2031340445 @default.
- W3005822432 cites W2034365378 @default.
- W3005822432 cites W2037743006 @default.
- W3005822432 cites W2042717860 @default.
- W3005822432 cites W2044840212 @default.
- W3005822432 cites W2048596671 @default.
- W3005822432 cites W2058379677 @default.
- W3005822432 cites W2073481677 @default.
- W3005822432 cites W2076503830 @default.
- W3005822432 cites W2095480650 @default.
- W3005822432 cites W2099280476 @default.
- W3005822432 cites W2100078415 @default.
- W3005822432 cites W2112886230 @default.
- W3005822432 cites W2130011291 @default.
- W3005822432 cites W2132065459 @default.
- W3005822432 cites W2147070662 @default.
- W3005822432 cites W2148833420 @default.
- W3005822432 cites W2151451423 @default.
- W3005822432 cites W2161000731 @default.
- W3005822432 cites W2163759087 @default.
- W3005822432 cites W2172573144 @default.
- W3005822432 cites W2417203243 @default.
- W3005822432 cites W2559520101 @default.
- W3005822432 cites W2582855915 @default.
- W3005822432 cites W2600305956 @default.
- W3005822432 cites W2736761791 @default.
- W3005822432 cites W2784114096 @default.
- W3005822432 cites W2792188161 @default.
- W3005822432 cites W2794314732 @default.
- W3005822432 cites W2910649574 @default.
- W3005822432 doi "https://doi.org/10.1038/s41598-020-58968-7" @default.
- W3005822432 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7012816" @default.
- W3005822432 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32047221" @default.
- W3005822432 hasPublicationYear "2020" @default.
- W3005822432 type Work @default.
- W3005822432 sameAs 3005822432 @default.
- W3005822432 citedByCount "2" @default.
- W3005822432 countsByYear W30058224322022 @default.
- W3005822432 crossrefType "journal-article" @default.
- W3005822432 hasAuthorship W3005822432A5014889588 @default.
- W3005822432 hasAuthorship W3005822432A5016677423 @default.
- W3005822432 hasAuthorship W3005822432A5019391870 @default.
- W3005822432 hasAuthorship W3005822432A5026869217 @default.
- W3005822432 hasAuthorship W3005822432A5028652883 @default.
- W3005822432 hasAuthorship W3005822432A5038112478 @default.
- W3005822432 hasAuthorship W3005822432A5044702979 @default.
- W3005822432 hasAuthorship W3005822432A5050470383 @default.
- W3005822432 hasAuthorship W3005822432A5066716873 @default.
- W3005822432 hasBestOaLocation W30058224321 @default.
- W3005822432 hasConcept C10389963 @default.
- W3005822432 hasConcept C104317684 @default.
- W3005822432 hasConcept C147483822 @default.
- W3005822432 hasConcept C159047783 @default.
- W3005822432 hasConcept C203014093 @default.
- W3005822432 hasConcept C2776460866 @default.
- W3005822432 hasConcept C2780115692 @default.
- W3005822432 hasConcept C2780861368 @default.
- W3005822432 hasConcept C2780868878 @default.
- W3005822432 hasConcept C54355233 @default.
- W3005822432 hasConcept C547475151 @default.
- W3005822432 hasConcept C60987743 @default.
- W3005822432 hasConcept C86803240 @default.
- W3005822432 hasConcept C8891405 @default.
- W3005822432 hasConcept C89423630 @default.
- W3005822432 hasConceptScore W3005822432C10389963 @default.
- W3005822432 hasConceptScore W3005822432C104317684 @default.
- W3005822432 hasConceptScore W3005822432C147483822 @default.
- W3005822432 hasConceptScore W3005822432C159047783 @default.
- W3005822432 hasConceptScore W3005822432C203014093 @default.
- W3005822432 hasConceptScore W3005822432C2776460866 @default.
- W3005822432 hasConceptScore W3005822432C2780115692 @default.
- W3005822432 hasConceptScore W3005822432C2780861368 @default.
- W3005822432 hasConceptScore W3005822432C2780868878 @default.
- W3005822432 hasConceptScore W3005822432C54355233 @default.
- W3005822432 hasConceptScore W3005822432C547475151 @default.
- W3005822432 hasConceptScore W3005822432C60987743 @default.
- W3005822432 hasConceptScore W3005822432C86803240 @default.
- W3005822432 hasConceptScore W3005822432C8891405 @default.