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- W3005829119 abstract "The migration of SiO2 nanoparticles (NPs) was investigated during agarose gel electrophoresis with various gel mesh sizes. As a model system, spherical SiO2 NPs of different sizes were synthesized by Stöber synthesis, and the surface was modified by covalently binding different types of ligands to tailor the ζ-potential as required. In the following step, agarose gels were cast with Tris/borate/EDTA (TBE) buffer solution, thereby controlling the mesh sizes by varying agarose concentrations, with higher concentrations leading to smaller mesh sizes. Additionally, the applied voltage could be varied for different electrophoretic experiments. Generally, large mesh sizes and high applied voltages lead to faster particle migration. The migration of SiO2 NPs occurs under two regimes: restricted migration, where small mesh size interferes with large particles so that the gel is acting similar to a sieve; and unrestricted migration, where the mesh size does not influence the particles’ migration behavior and they move according to their ζ-potential. Following these regimes, two different mechanisms of NP separation were possible inside agarose gels. Both mechanisms were used to separate binary mixtures into the former individual fractions, as proven by scanning electron microscopy (SEM) or the ninhydrin test." @default.
- W3005829119 created "2020-02-24" @default.
- W3005829119 creator A5026194056 @default.
- W3005829119 creator A5081969138 @default.
- W3005829119 date "2020-02-12" @default.
- W3005829119 modified "2023-10-12" @default.
- W3005829119 title "Restricted and Unrestricted Migration Mechanisms of Silica Nanoparticles in Agarose Gels and Their Utilization for the Separation of Binary Mixtures" @default.
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- W3005829119 doi "https://doi.org/10.1021/acs.jpcc.9b10644" @default.
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