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- W3005923209 abstract "The study aimed to test the potential for bone marrow stem cells (BMSC) mobilized by granulocyte macrophage colony stimulating factor (GM-CSF) to promote neovascularization and cardiomyocytes regeneration in a rat model of myocardial infarction. The myocardial infracted rats were randomly assigned to receive GM-CSF injection as GM-CSF group or received saline injection as control group. Evaluation of CD34+ stem cells was performed by flow cytometry. Cardiac functions were monitored using a multiple channel recorder via cardiac catheterization. Immunobiological staining including factor VIII and Ki67, and phosphotungstic acid-hematoxylin (PTAH) staining, were performed to assess angiogenesis and myogenesis and calculated myocardial infarction size. The CD34+ stem cells in blood and bone marrow of GM-CSF group increased significantlyon day 7 and day 14 comparing with control group, and declined on day 28. Immunobiological staining showed neovasculature formation and more Ki67 expression in the infracted regions of theGM-CSF group.Ki67 and PTAH double staining showed Ki67 positive signals were overlap with lymphocytes, fibroblasts and endothelial cells but not myocytes. No significant decrease of infracted size occurred in the GM-CSF group. These results suggested BMSC could be mobilized effectively by GM-CSF after myocardial infarction, which could only promote neovascularization without myogenesis." @default.
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- W3005923209 date "2017-01-01" @default.
- W3005923209 modified "2023-09-22" @default.
- W3005923209 title "Absence of myocyte regeneration by mobilization of bone marrow stem cells after myocardial infarction." @default.
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