Matches in SemOpenAlex for { <https://semopenalex.org/work/W3006329114> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W3006329114 endingPage "37" @default.
- W3006329114 startingPage "30" @default.
- W3006329114 abstract "Background and Objective: Poly-γ-glutamic acid (γ-PGA) is a constituent of the Bacillus anthracis capsule and a potential virulence factor of S. epidermidis . In this study, a methodology for the isolation, purification and quantification of γ-PGA in the isolates was adapted. In addition, the fate of the produced γ-PGA and its antiphagocytic activity were investigated. Methods: The capB gene was investigated by the PCR method in 50 isolates of S. epidermidis . A modified methodology was used for the extraction, purification, and quantification of γ-PGA using Cetyltrimethylammonium Bromide (CTAB) solution. The fate of γ-PGA was determined in Tryptic Soy Broth (TSB) medium, as well as the effect of ethanol, NaCl and KCl on the induction of the polymer production. The ability of neutrophils to phagocyte both FITC-labeled latex particles in the presence of free γ-PGA and S. epidermidis with and without anchored γ-PGA was evaluated by cytometry. Results: The production of γ-PGA was detected in 40 isolates; all of them were capB gene carriers. Free γ-PGA was detected and in the strain, the amount of released γ-PGA in the supernatant was 67% greater than the cell anchored γ-PGA. Phagocytosis tests performed with one γ-PGA producer isolate showed a significant reduction in neutrophil internalization. Conclusion: The adapted methodology was able to detect γ-PGA in the isolates studied. In addition to being found attached to the cell wall, it was demonstrated in this study that γ-PGA can also be found in the culture supernatant. Free γ-PGA did not determine a reduction in the internalization of latex by neutrophils, but cells with anchored γ-PGA showed significant protection against phagocytosis." @default.
- W3006329114 created "2020-02-24" @default.
- W3006329114 creator A5010915839 @default.
- W3006329114 creator A5013569079 @default.
- W3006329114 creator A5013578757 @default.
- W3006329114 creator A5032079144 @default.
- W3006329114 creator A5085420594 @default.
- W3006329114 date "2020-02-10" @default.
- W3006329114 modified "2023-09-25" @default.
- W3006329114 title "Production of Poly-γ-Glutamic Acid (γ-PGA) by Clinical Isolates of Staphylococcus Epidermidis" @default.
- W3006329114 cites W1487494898 @default.
- W3006329114 cites W1509202041 @default.
- W3006329114 cites W1979958088 @default.
- W3006329114 cites W2012539366 @default.
- W3006329114 cites W2023996751 @default.
- W3006329114 cites W2032686335 @default.
- W3006329114 cites W2034595187 @default.
- W3006329114 cites W2062374558 @default.
- W3006329114 cites W2104617674 @default.
- W3006329114 cites W2105512129 @default.
- W3006329114 cites W2115366678 @default.
- W3006329114 cites W2136514308 @default.
- W3006329114 cites W2143288882 @default.
- W3006329114 cites W2149443281 @default.
- W3006329114 cites W2156685415 @default.
- W3006329114 cites W2158977162 @default.
- W3006329114 cites W2335008813 @default.
- W3006329114 cites W2415074157 @default.
- W3006329114 cites W2468943383 @default.
- W3006329114 cites W2564149642 @default.
- W3006329114 cites W2773512002 @default.
- W3006329114 cites W2900027099 @default.
- W3006329114 cites W4239511728 @default.
- W3006329114 cites W4242084189 @default.
- W3006329114 cites W4252855438 @default.
- W3006329114 doi "https://doi.org/10.2174/1874285802014010030" @default.
- W3006329114 hasPublicationYear "2020" @default.
- W3006329114 type Work @default.
- W3006329114 sameAs 3006329114 @default.
- W3006329114 citedByCount "1" @default.
- W3006329114 countsByYear W30063291142022 @default.
- W3006329114 crossrefType "journal-article" @default.
- W3006329114 hasAuthorship W3006329114A5010915839 @default.
- W3006329114 hasAuthorship W3006329114A5013569079 @default.
- W3006329114 hasAuthorship W3006329114A5013578757 @default.
- W3006329114 hasAuthorship W3006329114A5032079144 @default.
- W3006329114 hasAuthorship W3006329114A5085420594 @default.
- W3006329114 hasBestOaLocation W30063291141 @default.
- W3006329114 hasConcept C104317684 @default.
- W3006329114 hasConcept C139770010 @default.
- W3006329114 hasConcept C1491633281 @default.
- W3006329114 hasConcept C185592680 @default.
- W3006329114 hasConcept C2776207055 @default.
- W3006329114 hasConcept C2777949923 @default.
- W3006329114 hasConcept C2779489039 @default.
- W3006329114 hasConcept C43617362 @default.
- W3006329114 hasConcept C523546767 @default.
- W3006329114 hasConcept C54355233 @default.
- W3006329114 hasConcept C55493867 @default.
- W3006329114 hasConcept C57409179 @default.
- W3006329114 hasConcept C60987743 @default.
- W3006329114 hasConcept C86803240 @default.
- W3006329114 hasConcept C89423630 @default.
- W3006329114 hasConceptScore W3006329114C104317684 @default.
- W3006329114 hasConceptScore W3006329114C139770010 @default.
- W3006329114 hasConceptScore W3006329114C1491633281 @default.
- W3006329114 hasConceptScore W3006329114C185592680 @default.
- W3006329114 hasConceptScore W3006329114C2776207055 @default.
- W3006329114 hasConceptScore W3006329114C2777949923 @default.
- W3006329114 hasConceptScore W3006329114C2779489039 @default.
- W3006329114 hasConceptScore W3006329114C43617362 @default.
- W3006329114 hasConceptScore W3006329114C523546767 @default.
- W3006329114 hasConceptScore W3006329114C54355233 @default.
- W3006329114 hasConceptScore W3006329114C55493867 @default.
- W3006329114 hasConceptScore W3006329114C57409179 @default.
- W3006329114 hasConceptScore W3006329114C60987743 @default.
- W3006329114 hasConceptScore W3006329114C86803240 @default.
- W3006329114 hasConceptScore W3006329114C89423630 @default.
- W3006329114 hasIssue "1" @default.
- W3006329114 hasLocation W30063291141 @default.
- W3006329114 hasOpenAccess W3006329114 @default.
- W3006329114 hasPrimaryLocation W30063291141 @default.
- W3006329114 hasRelatedWork W1594987181 @default.
- W3006329114 hasRelatedWork W1838684040 @default.
- W3006329114 hasRelatedWork W1996121325 @default.
- W3006329114 hasRelatedWork W2034359773 @default.
- W3006329114 hasRelatedWork W2067863020 @default.
- W3006329114 hasRelatedWork W2093808906 @default.
- W3006329114 hasRelatedWork W2611477722 @default.
- W3006329114 hasRelatedWork W3164964059 @default.
- W3006329114 hasRelatedWork W3213025941 @default.
- W3006329114 hasRelatedWork W2186532395 @default.
- W3006329114 hasVolume "14" @default.
- W3006329114 isParatext "false" @default.
- W3006329114 isRetracted "false" @default.
- W3006329114 magId "3006329114" @default.
- W3006329114 workType "article" @default.