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- W3006647641 abstract "The bacterium enterohemorrhagic Escherichia coli (EHEC) is one of the maincauses of food borne illness. The toxin of this bacteria is shiga toxin (STx) whichinhibits the protein biosynthesis of cells. Approaching the cells B-subunit of STx(STxB) binds to the receptor lipid globotriaosyl ceramide (Gb3) which is heterogeneousdistributed in the outer membrane of human cells. Such a cellular membrane isitself very heterogeneous and has regions of higher and lower ordering which influencesGb3 distribution. Furthermore, the cellular membrane is heterogeneously connectedeither to the cytoskeleton in the inside or to the surfaces on the outside of the cell,inducing different adhesional properties in the cell membrane (adhered membranesand non-adhered membranes). As postulated in a theory of Lipowsky et al.[1] this isassumed to influence ordering and lipid distribution in lipid membranes.In this work, the phase distribution of the Gb3 is analyzed in biomimetic modelmembranes as a function of the creamide backbone harboring different fatty acids.Fatty acid labeled Gb3s showed unnatural phase preference of STxB Gb3 binding.Fluorescently head group labeled Gb3s were investigated to study the influence ofdifferent fatty acids in liquid ordered (lo)/liquid disordered (ld) phase-separated giantunilamellar vesicles. Gb3s with the saturated fatty acid is preferentially distributedin the lo phase, while the Gb3s with the unsaturated fatty acid prefer the ld phase.An a-hydroxylation at the fatty acid had no effect of the distribution of Gb3. Incontrast different sphingomyelins (SMs) influence the partitioning of Gb3.The influence of heterogeneous membrane adhesion to biomimic membranes wasanalyzed using pore-spanning membranes (PSMs) on SiOx functionalized substrates.The heterogeneous membrane adhesion influences the measured phase diagram. Amembrane composed of DOPC/SM/cholesterol (Chol) (1:1:2) is still phase-separatedwith the ld phase in the solid supported PSM (s-PSM) and the lo phase in thefreestanding PSM (f-PSM). Phase diagrams of homogeneous membrane adhesion werereported to have no phase separation at the same Chol content which verifies thetheory of Lipowsky et al. for the analyzed lipid compositions." @default.
- W3006647641 created "2020-02-24" @default.
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- W3006647641 date "2019-12-11" @default.
- W3006647641 modified "2023-09-27" @default.
- W3006647641 title "Phase separation of biomimetic membranes" @default.
- W3006647641 hasPublicationYear "2019" @default.
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