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• W3006682340 endingPage "112111" @default.
• W3006682340 startingPage "112111" @default.
• W3006682340 abstract "Electrochemical biosensors possess numerous desirable qualities for target detection, such as portability and ease of use, and are often considered for point-of-care (POC) development. Label-free affinity electrochemical biosensors constructed with semiconductor manufacturing technology (SMT)-produced electrodes and a streptavidin biomediator currently display the highest reproducibility, accuracy, and stability in modern biosensors. However, such biosensors still do not meet POC guidelines regarding these three characteristics. The purpose of this research was to resolve the limitations in reproducibility and accuracy caused by problems with production of the biosensors, with the aim of developing a platform capable of producing devices that exceed POC standards. SMT production settings were optimized and bioreceptor immobilization was improved through the use of a unique linker, producing a biosensor with exceptional reproducibility, impressive accuracy, and high stability. Importantly, the three characteristics of the sensors produced using the proposed platform all meet POC standards set by the Clinical and Laboratory Standards Institute (CLSI). This suggests possible approval of the biosensors for POC development. Furthermore, the detection range of the platform was demonstrated by constructing biosensors capable of detecting common POC targets, including circulating tumor cells (CTCs), DNA/RNA, and curcumin, and the devices were optimized for POC use. Overall, the platform developed in this study shows high potential for production of POC biosensors." @default.
• W3006682340 created "2020-03-06" @default.
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• W3006682340 date "2020-05-01" @default.
• W3006682340 modified "2023-10-17" @default.
• W3006682340 title "Improving the reproducibility, accuracy, and stability of an electrochemical biosensor platform for point-of-care use" @default.
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• W3006682340 doi "https://doi.org/10.1016/j.bios.2020.112111" @default.
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