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• W3007039758 abstract "La sífilis es una infección de transmisión sexual causada por Treponema pallidum subsp. pallidum, cuya incidencia está aumentando en España y en el resto del mundo. El diagnóstico se basa fundamentalmente en la serología, puesto que el diagnóstico directo mediante microscopía de campo oscuro presenta dificultades que limitan su generalización. Las técnicas de biología molecular pueden ser una herramienta útil para el diagnóstico en sífilis primaria y secundaria, si bien no todos los tipos de muestra se comportan igual. También son útiles para el diagnóstico de la sífilis congénita, mientras que para la neurosífilis, y debido a la baja sensibilidad de la reacción en cadena de la polimerasa en el líquido cefalorraquídeo, no se recomiendan. Estás técnicas se han empleado para estudiar el controvertido origen de la sífilis, y mediante el sistema mejorado de los Centers for Disease Control and Prevention para realizar la tipificación que ayude a comprender mejor la epidemiología. Por último, las técnicas moleculares permiten determinar la presencia de mutaciones relacionadas con resistencia a los macrólidos, presentes en un porcentaje muy elevado de las infecciones. Syphilis is a sexually transmitted infection caused by Treponema pallidum subsp. pallidum with an increasing incidence in Spain and in the rest of the world. Diagnosis is based mainly on serology, since direct diagnosis by dark field microscopy presents difficulties that limit its widespread use. Molecular biology techniques can be a useful tool for diagnosis in primary and secondary syphilis, although not all types of samples show the same behaviour. These techniques are also useful for the diagnosis of congenital syphilis. They are not recommended, however, for neurosyphilis, due to the low sensitivity of polymerase chain reaction in cerebrospinal fluid. These techniques have been used to study the controversial origin of syphilis, and, through the enhanced Centers for Disease Control method, to perform typing, which helps to elucidate the epidemiology of this infection. Finally, molecular techniques can detect mutations related to macrolide resistance, which are present in a very high percentage of infections." @default.
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• W3007039758 date "2020-01-01" @default.
• W3007039758 modified "2023-10-02" @default.
• W3007039758 title "Diagnóstico molecular de la sífilis" @default.
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• W3007039758 doi "https://doi.org/10.1016/j.eimc.2020.02.002" @default.
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