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• W3007077233 endingPage "212" @default.
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• W3007077233 abstract "Abstract Trans ‐10, cis ‐12 conjugated linoleic acid (CLA) is a potent inhibitor of milk fat synthesis in the cow and similarly reduces milk fat in rodents. The objective of this study was to determine whether dietary fat can overcome CLA inhibition of milk fat concentration in lactating mice. Wild type C57Bl/6J mice ( n = 31) were fed semipurified diets containing either low fat (LF; 4% fat) or high fat (HF; 23.6% fat) starting 4–6 days postpartum. Dietary fat was increased by inclusion of high oleic sunflower oil. After 2 days on the experimental diets, lactating dams were orally dosed with either water (control) or trans ‐10, cis ‐12 CLA (20 mg/day) for 5 days. CLA treatment decreased pup growth similarly in both HF and LF diets. Milk fat percent was increased over 16% by the HF diet and decreased over 12% by CLA, but there was no interaction of dietary fat and CLA. Both CLA and the HF diet reduced the proportion of short‐ and medium‐chain fatty acids that originate from de novo synthesis, and there was no interaction of diet and CLA. CLA had no effect on the percent of preformed fatty acids, but the HF diet increased their abundance. Dietary fat and CLA both modified mammary expression of lipogenic enzymes and regulators, but no interactions were observed. In conclusion, CLA reduced milk fat concentration and litter growth, but these effects were not overcome by increased dietary fat from high oleic sunflower oil. CLA inhibition of milk fat in the mammary gland is not substrate dependent, and the mechanism is independent from dietary supply of oleic acid." @default.
• W3007077233 created "2020-03-06" @default.
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• W3007077233 date "2020-02-24" @default.
• W3007077233 modified "2023-09-27" @default.
• W3007077233 title "Dietary Fat Does Not Overcome<i>trans</i>‐10,<i>cis</i>‐12 Conjugated Linoleic Acid Inhibition of Milk Fat Synthesis in Lactating mice" @default.
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• W3007077233 doi "https://doi.org/10.1002/lipd.12228" @default.
• W3007077233 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32092162" @default.
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