FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3007452950> ?p ?o ?g. }

• W3007452950 abstract "Abstract Phosphorylation of Munc18-1 ( Stxbp1 ), a presynaptic organizer of synaptic vesicle fusion, is a powerful mechanism to regulate synaptic strength. Munc18-1 is a proposed substrate for the Down Syndrome-related kinase dual-specificity tyrosine phosphorylation-regulate kinase 1a ( Dyrk1a ) and mutations in both genes cause intellectual disability. However, the functional consequences of Dyrk1a-dependent phosphorylation of Munc18-1 for synapse function are unknown. Here, we show that the proposed Munc18-1 phosphorylation site, T479, is among the highly constrained phosphorylation sites in the coding regions of the gene and is also located within a larger constrained coding region. We confirm that Dyrk1a phosphorylates Munc18-1 at T479. Patch-clamp physiology in conditional null mutant hippocampal neurons expressing Cre and either wildtype, or mutants mimicking or preventing phosphorylation, revealed that synaptic transmission is similar among the three groups: frequency/amplitude of mEPSCs, evoked EPSCs, paired pulse plasticity, rundown kinetics upon intense activity and the readily releasable pool. However, synapses expressing the phosphomimic mutant responded to intense activity with more pronounced facilitation. These data indicate that Dyrk1a-dependent Munc18-1 phosphorylation has a minor impact on synaptic transmission, only after intense activity, and that the role of genetic variation in both genes in intellectual disability may be through different mechanisms." @default.
• W3007452950 created "2020-03-06" @default.
• W3007452950 creator A5026872836 @default.
• W3007452950 creator A5043321849 @default.
• W3007452950 creator A5056409348 @default.
• W3007452950 creator A5061802355 @default.
• W3007452950 creator A5066246563 @default.
• W3007452950 date "2020-02-21" @default.
• W3007452950 modified "2023-10-10" @default.
• W3007452950 title "A Munc18-1 mutant mimicking phosphorylation by Down Syndrome-related kinase Dyrk1a supports normal synaptic transmission and promotes recovery after intense activity" @default.
• W3007452950 cites W1602183564 @default.
• W3007452950 cites W1608240838 @default.
• W3007452950 cites W1822005186 @default.
• W3007452950 cites W1964178482 @default.
• W3007452950 cites W1978701742 @default.
• W3007452950 cites W1979702680 @default.
• W3007452950 cites W1980815431 @default.
• W3007452950 cites W1986729183 @default.
• W3007452950 cites W1987428952 @default.
• W3007452950 cites W1987907723 @default.
• W3007452950 cites W2002095454 @default.
• W3007452950 cites W2004602452 @default.
• W3007452950 cites W2007210656 @default.
• W3007452950 cites W2009761723 @default.
• W3007452950 cites W2015658471 @default.
• W3007452950 cites W2016516993 @default.
• W3007452950 cites W2019592149 @default.
• W3007452950 cites W2027520723 @default.
• W3007452950 cites W2036384121 @default.
• W3007452950 cites W2043274355 @default.
• W3007452950 cites W2044935263 @default.
• W3007452950 cites W2050776234 @default.
• W3007452950 cites W2051988683 @default.
• W3007452950 cites W2055103159 @default.
• W3007452950 cites W2067354100 @default.
• W3007452950 cites W2069389312 @default.
• W3007452950 cites W2070842726 @default.
• W3007452950 cites W2073032316 @default.
• W3007452950 cites W2106598300 @default.
• W3007452950 cites W2108994842 @default.
• W3007452950 cites W2109823971 @default.
• W3007452950 cites W2112181124 @default.
• W3007452950 cites W2118410873 @default.
• W3007452950 cites W2123347941 @default.
• W3007452950 cites W2128649861 @default.
• W3007452950 cites W2132849964 @default.
• W3007452950 cites W2135461141 @default.
• W3007452950 cites W2139268921 @default.
• W3007452950 cites W2141315977 @default.
• W3007452950 cites W2143060505 @default.
• W3007452950 cites W2147154230 @default.
• W3007452950 cites W2158456505 @default.
• W3007452950 cites W2160532124 @default.
• W3007452950 cites W2167791125 @default.
• W3007452950 cites W2170349574 @default.
• W3007452950 cites W2314723091 @default.
• W3007452950 cites W2341584309 @default.
• W3007452950 cites W2587764664 @default.
• W3007452950 cites W2603594400 @default.
• W3007452950 cites W2772776818 @default.
• W3007452950 cites W2791913816 @default.
• W3007452950 cites W2892826525 @default.
• W3007452950 cites W2952766192 @default.
• W3007452950 doi "https://doi.org/10.1038/s41598-020-59757-y" @default.
• W3007452950 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7035266" @default.
• W3007452950 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32081899" @default.
• W3007452950 hasPublicationYear "2020" @default.
• W3007452950 type Work @default.
• W3007452950 sameAs 3007452950 @default.
• W3007452950 citedByCount "3" @default.
• W3007452950 countsByYear W30074529502023 @default.
• W3007452950 crossrefType "journal-article" @default.
• W3007452950 hasAuthorship W3007452950A5026872836 @default.
• W3007452950 hasAuthorship W3007452950A5043321849 @default.
• W3007452950 hasAuthorship W3007452950A5056409348 @default.
• W3007452950 hasAuthorship W3007452950A5061802355 @default.
• W3007452950 hasAuthorship W3007452950A5066246563 @default.
• W3007452950 hasBestOaLocation W30074529501 @default.
• W3007452950 hasConcept C11960822 @default.
• W3007452950 hasConcept C127445978 @default.
• W3007452950 hasConcept C130316041 @default.
• W3007452950 hasConcept C148785051 @default.
• W3007452950 hasConcept C169760540 @default.
• W3007452950 hasConcept C170493617 @default.
• W3007452950 hasConcept C184235292 @default.
• W3007452950 hasConcept C200170125 @default.
• W3007452950 hasConcept C2777553839 @default.
• W3007452950 hasConcept C2777736865 @default.
• W3007452950 hasConcept C41625074 @default.
• W3007452950 hasConcept C55493867 @default.
• W3007452950 hasConcept C86803240 @default.
• W3007452950 hasConcept C95444343 @default.
• W3007452950 hasConcept C98229152 @default.
• W3007452950 hasConceptScore W3007452950C11960822 @default.
• W3007452950 hasConceptScore W3007452950C127445978 @default.
• W3007452950 hasConceptScore W3007452950C130316041 @default.
• W3007452950 hasConceptScore W3007452950C148785051 @default.
• W3007452950 hasConceptScore W3007452950C169760540 @default.
• W3007452950 hasConceptScore W3007452950C170493617 @default.
• W3007452950 hasConceptScore W3007452950C184235292 @default.

• next