Matches in SemOpenAlex for { <https://semopenalex.org/work/W3007516517> ?p ?o ?g. }
- W3007516517 endingPage "530" @default.
- W3007516517 startingPage "521" @default.
- W3007516517 abstract "The low cellular uptake of Methotrexate (MTX), a commonly used anticancer drug, is a big challenge for efficient cancer therapy. Self-assembled peptide nanoparticles (SAPNs) are one of the major classes of peptide vectors that have gained much attention toward novel drug delivery systems. In the present study, different sequences of cell-penetrating peptides including R2W4R2 and W3R4W3 and their SAPNs (R2W4R2-E12 and W3R4W3-E12) were designed for efficient delivery of MTX into MCF7 breast cancer cells. Based on electron microscopy results, the obtained SAPNs were in nano scale with spherical shape. There was a positive relationship between the free energy of water to octanol transferring and cellular penetration of designed nanostructures. The R2W4R2 possessed proper free energy and ability to form a spherical structure and hydrophobic–hydrophobic interactions, therefore, exhibited more cellular penetration than W3R4W3. The cellular uptake of obtained nanoparticles was examined by flow cytometry and fluorescence microscopy, in which, R2W4R2 and R2W4R2-E12 showed more appropriate penetration into MCF7 cells than W3R4W3 and W3R4W3-E12. The cytotoxicity of MTX-loaded peptides and SAPNs was examined by MTT assay. As a result, at higher concentrations, the R2W4R2 and R2W4R2-E12 showed higher cytotoxic behavior than their counterparts. Despite their enhanced cellular internalization, the cytotoxic behavior of MTX-loaded SAPNs at lower concentrations was relatively less than free MTX, which could be ascribed to the gradual nature of drug detachment from these conjugates. Therefore, R2W4R2 could be considered as an efficient choice to enhance the therapeutic efficiency of MTX in cancer treatments." @default.
- W3007516517 created "2020-03-06" @default.
- W3007516517 creator A5003793995 @default.
- W3007516517 creator A5009670303 @default.
- W3007516517 creator A5016322017 @default.
- W3007516517 creator A5023571572 @default.
- W3007516517 creator A5029131650 @default.
- W3007516517 creator A5039593668 @default.
- W3007516517 creator A5042268259 @default.
- W3007516517 creator A5047204000 @default.
- W3007516517 creator A5056098336 @default.
- W3007516517 creator A5061666481 @default.
- W3007516517 date "2020-03-05" @default.
- W3007516517 modified "2023-10-18" @default.
- W3007516517 title "Self-assembled peptide nanoparticles for efficient delivery of methotrexate into cancer cells" @default.
- W3007516517 cites W1585436247 @default.
- W3007516517 cites W1973322743 @default.
- W3007516517 cites W1976915573 @default.
- W3007516517 cites W1994579473 @default.
- W3007516517 cites W1995578361 @default.
- W3007516517 cites W2007248387 @default.
- W3007516517 cites W2013438185 @default.
- W3007516517 cites W2015338160 @default.
- W3007516517 cites W2016544346 @default.
- W3007516517 cites W2026593599 @default.
- W3007516517 cites W2035558311 @default.
- W3007516517 cites W2048146070 @default.
- W3007516517 cites W2050944546 @default.
- W3007516517 cites W2058738744 @default.
- W3007516517 cites W2069888185 @default.
- W3007516517 cites W2086992699 @default.
- W3007516517 cites W2087722290 @default.
- W3007516517 cites W2098021220 @default.
- W3007516517 cites W2101135540 @default.
- W3007516517 cites W2103128016 @default.
- W3007516517 cites W2104534420 @default.
- W3007516517 cites W2109437819 @default.
- W3007516517 cites W2141276842 @default.
- W3007516517 cites W2155094060 @default.
- W3007516517 cites W2265596469 @default.
- W3007516517 cites W2269922408 @default.
- W3007516517 cites W2409603104 @default.
- W3007516517 cites W2410232097 @default.
- W3007516517 cites W2440783819 @default.
- W3007516517 cites W2466983808 @default.
- W3007516517 cites W2568141451 @default.
- W3007516517 cites W2766932092 @default.
- W3007516517 cites W2769306974 @default.
- W3007516517 cites W2783614941 @default.
- W3007516517 cites W2790248187 @default.
- W3007516517 cites W2914518223 @default.
- W3007516517 cites W4239028853 @default.
- W3007516517 doi "https://doi.org/10.1080/03639045.2020.1734017" @default.
- W3007516517 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32116040" @default.
- W3007516517 hasPublicationYear "2020" @default.
- W3007516517 type Work @default.
- W3007516517 sameAs 3007516517 @default.
- W3007516517 citedByCount "6" @default.
- W3007516517 countsByYear W30075165172020 @default.
- W3007516517 countsByYear W30075165172021 @default.
- W3007516517 countsByYear W30075165172022 @default.
- W3007516517 countsByYear W30075165172023 @default.
- W3007516517 crossrefType "journal-article" @default.
- W3007516517 hasAuthorship W3007516517A5003793995 @default.
- W3007516517 hasAuthorship W3007516517A5009670303 @default.
- W3007516517 hasAuthorship W3007516517A5016322017 @default.
- W3007516517 hasAuthorship W3007516517A5023571572 @default.
- W3007516517 hasAuthorship W3007516517A5029131650 @default.
- W3007516517 hasAuthorship W3007516517A5039593668 @default.
- W3007516517 hasAuthorship W3007516517A5042268259 @default.
- W3007516517 hasAuthorship W3007516517A5047204000 @default.
- W3007516517 hasAuthorship W3007516517A5056098336 @default.
- W3007516517 hasAuthorship W3007516517A5061666481 @default.
- W3007516517 hasConcept C109316439 @default.
- W3007516517 hasConcept C121332964 @default.
- W3007516517 hasConcept C121608353 @default.
- W3007516517 hasConcept C12554922 @default.
- W3007516517 hasConcept C127413603 @default.
- W3007516517 hasConcept C134306372 @default.
- W3007516517 hasConcept C139770010 @default.
- W3007516517 hasConcept C1491633281 @default.
- W3007516517 hasConcept C153911025 @default.
- W3007516517 hasConcept C154317977 @default.
- W3007516517 hasConcept C155672457 @default.
- W3007516517 hasConcept C169274487 @default.
- W3007516517 hasConcept C171250308 @default.
- W3007516517 hasConcept C178790620 @default.
- W3007516517 hasConcept C185592680 @default.
- W3007516517 hasConcept C192562407 @default.
- W3007516517 hasConcept C197336794 @default.
- W3007516517 hasConcept C202751555 @default.
- W3007516517 hasConcept C203014093 @default.
- W3007516517 hasConcept C2776929087 @default.
- W3007516517 hasConcept C2779178360 @default.
- W3007516517 hasConcept C2779281246 @default.
- W3007516517 hasConcept C2779820397 @default.
- W3007516517 hasConcept C2780783641 @default.
- W3007516517 hasConcept C2781059491 @default.