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- W3008218898 abstract "Abstract Bacillus megaterium is a spore forming bacteria that is mostly used as a probiotic in aquaculture. Spore formation for probiotic production using carbon source is costly. This research evaluated the effect of different carbon doses of tapioca ( Manihot esculenta ) flour on vegetative cells, spore production, sporulation efficiency and spore germination of B. megaterium . Experiments were carried out in Aquaculture Laboratory, University of Brawijaya. In flasks, 50 ml of growth media were used and were inoculated with 1% of B. megaterium (2.6 x 10 8 cells/ml). The cultures were mixed (120 rpm) and incubated at 37 °C for 120 hours with 4 different treatments of carbon doses (5, 10, 15 and 20 g/L, respectively). The results showed that the highest vegetative cells (8.4 x 10 7 cell/ml) and spore production (4.1 x 10 7 spore/ml) of B. megaterium were found at 15 g/L carbon dose. However, it was not followed by high sporulation efficiency (only 49.01%). The high spore germination was observed in more than 5 g/L carbon dose. Thus, 5-15 g/L carbon doses of tapioca flour could positively enhance vegetative cells, spore production, sporulation efficiency and spore germination of B. megaterium and could be used as a potential source of probiotics in aquaculture." @default.
- W3008218898 created "2020-03-06" @default.
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- W3008218898 date "2020-02-01" @default.
- W3008218898 modified "2023-09-26" @default.
- W3008218898 title "Effect of different carbon doses of tapioca (Manihot esculenta) flour on vegetative cells and spore production of Bacillus megaterium" @default.
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- W3008218898 doi "https://doi.org/10.1088/1755-1315/441/1/012106" @default.
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