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• W3008347455 endingPage "118195" @default.
• W3008347455 startingPage "118195" @default.
• W3008347455 abstract "Ficin has been reported to possess peroxidase activity, but its applications in some respects have been limited because of its relatively low activity. Herein, a mesoporous metal-organic framework, PCN-333(Fe), was synthesized, which was selected to encapsulate ficin to form [email protected](Fe). Compared with ficin, the peroxidase-like activity of [email protected](Fe) toward 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation was about 3 times increase in the presence of H2O2, and followed classical Michaelis-Menten model. The kinetic parameters showed that stronger affinity and higher catalytic constant (Kcat) of [email protected](Fe) to both TMB and H2O2 compared with ficin, and Kcat of [email protected](Fe) was increased by 3.65 folds and 3.59 folds for TMB and H2O2, respectively. Taking advantages of higher catalytic property of [email protected](Fe), we developed a colorimetric method with high sensitivity and selectivity to detect glucose, which displayed a good linear response toward glucose in the range of 0.5–180 μM with a limit of detection of 97 nM. Furthermore, [email protected](Fe) has been proven to successfully detect glucose in human serum, implying its great potentialities and wide applications as peroxidase mimics." @default.
• W3008347455 created "2020-03-06" @default.
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• W3008347455 date "2020-06-01" @default.
• W3008347455 modified "2023-10-16" @default.
• W3008347455 title "Ficin encapsulated in mesoporous metal-organic frameworks with enhanced peroxidase-like activity and colorimetric detection of glucose" @default.
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• W3008347455 doi "https://doi.org/10.1016/j.saa.2020.118195" @default.
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