FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3008704648> ?p ?o ?g. }

• W3008704648 endingPage "103658" @default.
• W3008704648 startingPage "103658" @default.
• W3008704648 abstract "NK-lysin is an important part of the innate immune defence system and plays an important role in resisting the invasion of pathogenic microorganisms. In this study, NK-lysin from golden pompano (Trachinotus ovatus) was characterized and its expression in response to Photobacterium damselae was investigated. The full-length NK-lysin cDNA was 731 bp, which comprised a 5′-UTR of 63 bp, an ORF of 444 bp, and a 3′-UTR of 224 bp, and encoded 147 amino acids; NK-lysin consisted of a conserved saposin B domain and six conserved cysteines that formed three pairs of disulfide bonds. The genomic organization of NK-lysin was also determined and the gene consisted of four introns and five exons. The predicted promoter region of ToNK-lysin contained several putative transcription factor binding sites. Quantitative real-time (qRT-PCR) analysis indicated that ToNK-lysin was ubiquitously expressed in all examined tissues; the highest mRNA levels were observed in the skin, kidney and intestine, while the lowest expression level was detected in the stomach. After P. damselae stimulation, the expression level of NK-lysin mRNA was significantly upregulated in various tissues of golden pompano. In addition, SDS-PAGE showed that the molecular mass of recombinant NK-lysin expressed in pGEX-6P-1 was approximately 37 kDa. The purified recombinant protein showed antibacterial activity against gram-positive and gram-negative bacteria. The results indicate that golden pompano NK-lysin has potential antimicrobial roles in fish innate immunity." @default.
• W3008704648 created "2020-03-06" @default.
• W3008704648 creator A5001843018 @default.
• W3008704648 creator A5005284448 @default.
• W3008704648 creator A5008212664 @default.
• W3008704648 creator A5009318195 @default.
• W3008704648 creator A5010939818 @default.
• W3008704648 creator A5054607033 @default.
• W3008704648 creator A5074546007 @default.
• W3008704648 creator A5076080012 @default.
• W3008704648 creator A5090815103 @default.
• W3008704648 date "2020-06-01" @default.
• W3008704648 modified "2023-10-17" @default.
• W3008704648 title "Functional characterization of NK-lysin in golden pompano Trachinotus ovatus (Linnaeus 1758)" @default.
• W3008704648 cites W1910115396 @default.
• W3008704648 cites W1963612215 @default.
• W3008704648 cites W1965993834 @default.
• W3008704648 cites W1967497464 @default.
• W3008704648 cites W1986965820 @default.
• W3008704648 cites W1999548513 @default.
• W3008704648 cites W2011666992 @default.
• W3008704648 cites W2011823468 @default.
• W3008704648 cites W2023720312 @default.
• W3008704648 cites W2031789714 @default.
• W3008704648 cites W2036163843 @default.
• W3008704648 cites W2037794503 @default.
• W3008704648 cites W2048730807 @default.
• W3008704648 cites W2050043512 @default.
• W3008704648 cites W2059940291 @default.
• W3008704648 cites W2062205282 @default.
• W3008704648 cites W2067957919 @default.
• W3008704648 cites W2077135198 @default.
• W3008704648 cites W2087873917 @default.
• W3008704648 cites W2094463786 @default.
• W3008704648 cites W2102065042 @default.
• W3008704648 cites W2107277218 @default.
• W3008704648 cites W2110899053 @default.
• W3008704648 cites W2113907907 @default.
• W3008704648 cites W2121874550 @default.
• W3008704648 cites W2140561118 @default.
• W3008704648 cites W2164725520 @default.
• W3008704648 cites W2168109012 @default.
• W3008704648 cites W2178786587 @default.
• W3008704648 cites W2188464224 @default.
• W3008704648 cites W2332486970 @default.
• W3008704648 cites W2401091899 @default.
• W3008704648 cites W2766713866 @default.
• W3008704648 cites W2767040228 @default.
• W3008704648 cites W2769664848 @default.
• W3008704648 cites W2799696670 @default.
• W3008704648 cites W2886725395 @default.
• W3008704648 cites W2897058203 @default.
• W3008704648 cites W2911874905 @default.
• W3008704648 cites W2913479683 @default.
• W3008704648 cites W2921040963 @default.
• W3008704648 cites W2936672112 @default.
• W3008704648 cites W2969133655 @default.
• W3008704648 cites W2969405948 @default.
• W3008704648 doi "https://doi.org/10.1016/j.dci.2020.103658" @default.
• W3008704648 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32087193" @default.
• W3008704648 hasPublicationYear "2020" @default.
• W3008704648 type Work @default.
• W3008704648 sameAs 3008704648 @default.
• W3008704648 citedByCount "14" @default.
• W3008704648 countsByYear W30087046482020 @default.
• W3008704648 countsByYear W30087046482021 @default.
• W3008704648 countsByYear W30087046482022 @default.
• W3008704648 countsByYear W30087046482023 @default.
• W3008704648 crossrefType "journal-article" @default.
• W3008704648 hasAuthorship W3008704648A5001843018 @default.
• W3008704648 hasAuthorship W3008704648A5005284448 @default.
• W3008704648 hasAuthorship W3008704648A5008212664 @default.
• W3008704648 hasAuthorship W3008704648A5009318195 @default.
• W3008704648 hasAuthorship W3008704648A5010939818 @default.
• W3008704648 hasAuthorship W3008704648A5054607033 @default.
• W3008704648 hasAuthorship W3008704648A5074546007 @default.
• W3008704648 hasAuthorship W3008704648A5076080012 @default.
• W3008704648 hasAuthorship W3008704648A5090815103 @default.
• W3008704648 hasConcept C104317684 @default.
• W3008704648 hasConcept C105580179 @default.
• W3008704648 hasConcept C136449434 @default.
• W3008704648 hasConcept C153911025 @default.
• W3008704648 hasConcept C187882448 @default.
• W3008704648 hasConcept C22538091 @default.
• W3008704648 hasConcept C2776441376 @default.
• W3008704648 hasConcept C40767141 @default.
• W3008704648 hasConcept C54355233 @default.
• W3008704648 hasConcept C547475151 @default.
• W3008704648 hasConcept C86803240 @default.
• W3008704648 hasConcept C8891405 @default.
• W3008704648 hasConcept C89423630 @default.
• W3008704648 hasConcept C89604277 @default.
• W3008704648 hasConceptScore W3008704648C104317684 @default.
• W3008704648 hasConceptScore W3008704648C105580179 @default.
• W3008704648 hasConceptScore W3008704648C136449434 @default.
• W3008704648 hasConceptScore W3008704648C153911025 @default.
• W3008704648 hasConceptScore W3008704648C187882448 @default.
• W3008704648 hasConceptScore W3008704648C22538091 @default.

• next