FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3009173812> ?p ?o ?g. }

• W3009173812 abstract "Abstract Mycobacterium tuberculosis ( M.tb ) causes the current leading infectious disease. Examination of the functional genomics of M.tb and development of drugs and vaccines are hampered by the complicated and time-consuming genetic manipulation techniques for M.tb. Here, we reprogrammed M.tb endogenous type III-A CRISPR-Cas10 system for simple and efficient gene editing, RNA interference and screening via simple delivery of a plasmid harboring a mini-CRISPR array, thereby avoiding the introduction of exogenous proteins and minimizing proteotoxicity. We demonstrated that M.tb genes were efficiently and specifically knocked-in/out by this system, which was confirmed by whole-genome sequencing. This system was further employed for single and simultaneous multiple-gene RNA interference. Moreover, we successfully applied this system for genome-wide CRISPR interference screening to identify the in-vitro and intracellular growth-regulating genes. This system can be extensively used to explore the functional genomics of M.tb and facilitate the development of new anti- Mycobacterial drugs and vaccines. Summary Tuberculosis caused by Mycobacterium tuberculosis ( M.tb ) is the current leading infectious disease affecting more than ten million people annually. To dissect the functional genomics and understand its virulence, persistence, and antibiotics resistance, a powerful genome editing tool and high-throughput screening methods are desperately wanted. Our study developed an efficient and a robust tool for genome editing and RNA interference in M.tb using its endogenous CRISPR cas10 system. Moreover, the system has been successfully applied for genome-wide CRISPR interference screening. This tool could be employed to explore the functional genomics of M.tb and facilitate the development of anti- M.tb drugs and vaccines." @default.
• W3009173812 created "2020-03-13" @default.
• W3009173812 creator A5008171524 @default.
• W3009173812 creator A5010436871 @default.
• W3009173812 creator A5015878350 @default.
• W3009173812 creator A5030980350 @default.
• W3009173812 creator A5033494854 @default.
• W3009173812 creator A5034524285 @default.
• W3009173812 creator A5052030148 @default.
• W3009173812 creator A5055072758 @default.
• W3009173812 creator A5068146252 @default.
• W3009173812 creator A5075857347 @default.
• W3009173812 creator A5080196425 @default.
• W3009173812 creator A5080403361 @default.
• W3009173812 creator A5082598946 @default.
• W3009173812 creator A5083122800 @default.
• W3009173812 creator A5086818276 @default.
• W3009173812 creator A5087956762 @default.
• W3009173812 creator A5088344976 @default.
• W3009173812 date "2020-03-09" @default.
• W3009173812 modified "2023-09-23" @default.
• W3009173812 title "Reprogramming the endogenous type III-A CRISPR-Cas system for genome editing, RNA interference and CRISPRi screening in <i>Mycobacterium tuberculosis</i>" @default.
• W3009173812 cites W1514631861 @default.
• W3009173812 cites W1517221380 @default.
• W3009173812 cites W1554484593 @default.
• W3009173812 cites W1931234589 @default.
• W3009173812 cites W1934542416 @default.
• W3009173812 cites W1977531433 @default.
• W3009173812 cites W1994145955 @default.
• W3009173812 cites W2000046950 @default.
• W3009173812 cites W2000652167 @default.
• W3009173812 cites W2005548636 @default.
• W3009173812 cites W2017173637 @default.
• W3009173812 cites W2024880741 @default.
• W3009173812 cites W2048071312 @default.
• W3009173812 cites W2055252390 @default.
• W3009173812 cites W2067576392 @default.
• W3009173812 cites W2074231493 @default.
• W3009173812 cites W2083159789 @default.
• W3009173812 cites W2083632051 @default.
• W3009173812 cites W2084467121 @default.
• W3009173812 cites W2101830136 @default.
• W3009173812 cites W2102545353 @default.
• W3009173812 cites W2102619694 @default.
• W3009173812 cites W2103110885 @default.
• W3009173812 cites W2103441770 @default.
• W3009173812 cites W2107959600 @default.
• W3009173812 cites W2108234281 @default.
• W3009173812 cites W2118755041 @default.
• W3009173812 cites W2119180969 @default.
• W3009173812 cites W2120503144 @default.
• W3009173812 cites W2120651372 @default.
• W3009173812 cites W2125057177 @default.
• W3009173812 cites W2125301421 @default.
• W3009173812 cites W2127631899 @default.
• W3009173812 cites W2128482141 @default.
• W3009173812 cites W2128669068 @default.
• W3009173812 cites W2131271579 @default.
• W3009173812 cites W2136609905 @default.
• W3009173812 cites W2140354550 @default.
• W3009173812 cites W2152239989 @default.
• W3009173812 cites W2159386537 @default.
• W3009173812 cites W2161366481 @default.
• W3009173812 cites W2163241233 @default.
• W3009173812 cites W2165539745 @default.
• W3009173812 cites W2274320127 @default.
• W3009173812 cites W2303243520 @default.
• W3009173812 cites W2304692062 @default.
• W3009173812 cites W2305032637 @default.
• W3009173812 cites W2341289363 @default.
• W3009173812 cites W2469203838 @default.
• W3009173812 cites W2536485573 @default.
• W3009173812 cites W2574984917 @default.
• W3009173812 cites W2586637020 @default.
• W3009173812 cites W2601854228 @default.
• W3009173812 cites W2623277795 @default.
• W3009173812 cites W2732924043 @default.
• W3009173812 cites W2734470795 @default.
• W3009173812 cites W2752362658 @default.
• W3009173812 cites W2775420542 @default.
• W3009173812 cites W2794376461 @default.
• W3009173812 cites W2808800351 @default.
• W3009173812 cites W2812187546 @default.
• W3009173812 cites W2884607607 @default.
• W3009173812 cites W2886269807 @default.
• W3009173812 cites W2915650154 @default.
• W3009173812 cites W2917714207 @default.
• W3009173812 cites W2940877588 @default.
• W3009173812 cites W2949787845 @default.
• W3009173812 cites W2953131670 @default.
• W3009173812 cites W2968452410 @default.
• W3009173812 cites W4235939910 @default.
• W3009173812 doi "https://doi.org/10.1101/2020.03.09.983494" @default.
• W3009173812 hasPublicationYear "2020" @default.
• W3009173812 type Work @default.
• W3009173812 sameAs 3009173812 @default.
• W3009173812 citedByCount "4" @default.
• W3009173812 countsByYear W30091738122020 @default.
• W3009173812 countsByYear W30091738122021 @default.
• W3009173812 countsByYear W30091738122022 @default.

• next