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- W3009259600 abstract "Abstract The incidence of intervertebral disc (IVD) degeneration disease, caused by changes in the osmotic pressure of nucleus pulposus (NP) cells, increases with age. In general, low back pain is associated with IVD degeneration. However, the mechanism and molecular target of low back pain have not been elucidated, and there are no data suggesting specific biomarkers of low back pain. Therefore, the research aims to identify and verify the significant gene biomarkers of low back pain. The differentially expressed genes (DEGs) were screened in the Gene Expression Omnibus (GEO) database, and the identification and analysis of significant gene biomarkers were also performed with various bioinformatics programs. A total of 120 patients with low back pain were recruited. Before surgery, the degree of pain was measured by the numeric rating scale (NRS), which enables comparison of the pain scores from individuals. After surgery, IVD tissues were obtained, and NP cells were isolated. The NP cells were cultured in two various osmotic media, including iso-osmotic media (293 mOsm/kg H 2 O) to account for the morbid environment of NP cells in IVD degeneration disease and hyper-osmotic media (450 mOsm/kg H 2 O) to account for the normal condition of NP cells in healthy individuals. The relative mRNA expression levels of CCL5, OPRL1, CXCL13, and SST were measured by quantitative real-time PCR in the in vitro analysis of the osmotic pressure experiments. Finally, correlation analysis and a neural network module were employed to explore the linkage between significant gene biomarkers and pain. A total of 371 DEGs were identified, including 128 downregulated genes and 243 upregulated genes. Furthermore, the four genes (CCL5, OPRL1, SST, and CXCL13) were identified as significant gene biomarkers of low back pain (P < 0.001) based on univariate linear regression, and CCL5 (odds ratio, 34.667; P = 0.003) and OPRL1 (odds ratio, 19.875; P < 0.001) were significantly related to low back pain through multivariate logistic regression. The expression of CCL5 and OPRL1 might be correlated with low back pain in patients with IVD degeneration disease caused by changes in the osmotic pressure of NP cells." @default.
- W3009259600 created "2020-03-13" @default.
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- W3009259600 date "2020-02-28" @default.
- W3009259600 modified "2023-09-29" @default.
- W3009259600 title "Identification of significant gene biomarkers of low back pain caused by changes in the osmotic pressure of nucleus pulposus cells" @default.
- W3009259600 cites W114536063 @default.
- W3009259600 cites W1523062047 @default.
- W3009259600 cites W1527933794 @default.
- W3009259600 cites W1543912755 @default.
- W3009259600 cites W1661652663 @default.
- W3009259600 cites W1968712129 @default.
- W3009259600 cites W1970509697 @default.
- W3009259600 cites W1973056823 @default.
- W3009259600 cites W1977849332 @default.
- W3009259600 cites W1984069777 @default.
- W3009259600 cites W1988029416 @default.
- W3009259600 cites W1988512128 @default.
- W3009259600 cites W1989077880 @default.
- W3009259600 cites W1989934761 @default.
- W3009259600 cites W2000027332 @default.
- W3009259600 cites W2002153331 @default.
- W3009259600 cites W2002945814 @default.
- W3009259600 cites W2006247426 @default.
- W3009259600 cites W2007413067 @default.
- W3009259600 cites W2009006906 @default.
- W3009259600 cites W2017988012 @default.
- W3009259600 cites W2022740921 @default.
- W3009259600 cites W2025043134 @default.
- W3009259600 cites W2030604088 @default.
- W3009259600 cites W2031116764 @default.
- W3009259600 cites W2031414838 @default.
- W3009259600 cites W2035168899 @default.
- W3009259600 cites W2035486012 @default.
- W3009259600 cites W2037806019 @default.
- W3009259600 cites W2048102871 @default.
- W3009259600 cites W2053411228 @default.
- W3009259600 cites W2060042889 @default.
- W3009259600 cites W2077857008 @default.
- W3009259600 cites W2082480800 @default.
- W3009259600 cites W2084656370 @default.
- W3009259600 cites W2085313497 @default.
- W3009259600 cites W2086199843 @default.
- W3009259600 cites W2090272540 @default.
- W3009259600 cites W2090321984 @default.
- W3009259600 cites W2090519904 @default.
- W3009259600 cites W2092493132 @default.
- W3009259600 cites W2092798278 @default.
- W3009259600 cites W2095297866 @default.
- W3009259600 cites W2096173332 @default.
- W3009259600 cites W2097795961 @default.
- W3009259600 cites W2114123652 @default.
- W3009259600 cites W2120256133 @default.
- W3009259600 cites W2123106337 @default.
- W3009259600 cites W2132173287 @default.
- W3009259600 cites W2136547076 @default.
- W3009259600 cites W2136850043 @default.
- W3009259600 cites W2137089630 @default.
- W3009259600 cites W2146512944 @default.
- W3009259600 cites W2149905759 @default.
- W3009259600 cites W2152920456 @default.
- W3009259600 cites W2155142785 @default.
- W3009259600 cites W2160018962 @default.
- W3009259600 cites W2166016039 @default.
- W3009259600 cites W2167561374 @default.
- W3009259600 cites W2210603493 @default.
- W3009259600 cites W2234168863 @default.
- W3009259600 cites W2343179175 @default.
- W3009259600 cites W2418558247 @default.
- W3009259600 cites W2472601390 @default.
- W3009259600 cites W2474088718 @default.
- W3009259600 cites W2475088686 @default.
- W3009259600 cites W2582897574 @default.
- W3009259600 cites W2589380060 @default.
- W3009259600 cites W2768516807 @default.
- W3009259600 cites W2796422959 @default.
- W3009259600 cites W2885064422 @default.
- W3009259600 cites W2889724656 @default.
- W3009259600 cites W2890864808 @default.
- W3009259600 cites W2912969594 @default.
- W3009259600 cites W2928665623 @default.
- W3009259600 cites W2941002319 @default.
- W3009259600 cites W2942955226 @default.
- W3009259600 cites W2950745849 @default.
- W3009259600 cites W2959190003 @default.
- W3009259600 cites W2971609214 @default.
- W3009259600 doi "https://doi.org/10.1038/s41598-020-60714-y" @default.
- W3009259600 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7048739" @default.
- W3009259600 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32111963" @default.
- W3009259600 hasPublicationYear "2020" @default.
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