FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3009288510> ?p ?o ?g. }

• W3009288510 endingPage "327" @default.
• W3009288510 startingPage "315" @default.
• W3009288510 abstract "El objetivo del presente estudio fue aislar y seleccionar bacterias endofíticas de arroz capaces de inhibir al fitopatógeno Burkholderia glumae THT, así como caracterizarlas por su genética y bioquímica. También se buscó caracterizar la diversidad genética y los factores de virulencia presentes en cepas de B. glumae y de Burkholderia gladioli, otro patógeno de arroz, aisladas de campo. Se colectaron plantas de arroz en 4 departamentos del norte de Perú, y tras la desinfección de tejidos se aislaron bacterias endofíticas por cultivo en agar soya tripticasa (30 °C; 48 h) y en medio selectivo (pH 4,5; 41 °C; 72 h). Se evaluó la actividad antimicrobiana frente a B. glumae THT, la producción de sideróforos y la resistencia a la toxoflavina, toxina producida por este agente. La identificación molecular se realizó mediante BOX-PCR y secuenciación del gen 16S ARNr. Además, se determinó la producción de enzimas extracelulares y se efectuaron ensayos de motilidad y sensibilidad/resistencia a bactericidas. Se aislaron 189 bacterias endofíticas, de las cuales solo 9 presentaron actividad antimicrobiana contra B. glumae THT, sobresaliendo Burkholderia vietnamiensis TUR04-01, B. vietnamiensis TUR04-03 y Bacillus aryabhattai AMH12-02. Estas cepas produjeron sideróforos y al menos el 55,5% fueron resistentes a la toxoflavina. Por otro lado, 17 de las cepas de B. glumae y B. gladioli aisladas se agruparon en 9 perfiles BOX-PCR, 16 de ellas presentaron similitud con B. glumae LMG2196T (100%) y una con B. gladioli NBRC13700T (99,86%). Hubo elevada diversidad de acuerdo al origen geográfico y se encontraron factores de virulencia. En conclusión, se hallaron cepas del género Bacillus y Burkholderia que podrían ser agentes de biocontrol contra B. glumae. The aim of the present study was to isolate, select and characterize endophytic bacteria in rice inhibiting Burkholderia glumae THT as well as to characterize the genetic diversity and virulence factors in strains of B. glumae and Burkholderia gladioli of rice. Rice plants were collected in 4 departments from the northern region of Peru, isolating endophytic bacteria, after tissue sterilization, at 30 °C (48 h) in Trypticase Soy Agar (TSA), evaluating the antimicrobial activity against B. glumae THT, production of siderophores, resistance of toxoflavine and partial sequencing of the 16S rRNA gene. Furthermore, B. glumae and B. gladioli were isolated in selective medium (pH 4.5) at 41 °C/72 h. Molecular identification was performed using BOX-PCR and sequencing of the 16S rRNA gene, in addition to the production of extracellular enzymes, motility tests and sensitivity/resistance to bactericides. One hundred and eighty nine (189) endophytic bacteria were isolated, and only 9 strains showed antimicrobial activity against B. glumae THT, highlighting Burkholderia vietnamiensis TUR04-01, B. vietnamiensis TUR04-03 and Bacillus aryabhattai AMH12-02. The strains produced siderophores and at least 55.5% were resistant to toxoflavin. Additionally, 17 strains were grouped into 9 BOX-PCR profiles, where 16 had similarity with B. glumae LMG2196T (100%) and 1 with B. gladioli NBRC 13700T (99.86%). High diversity was found according to geographical origin and virulence factors. In conclusion, strains of the genus Bacillus and Burkholderia are potential biocontrol agents against B. glumae." @default.
• W3009288510 created "2020-03-13" @default.
• W3009288510 creator A5013941919 @default.
• W3009288510 creator A5018196885 @default.
• W3009288510 creator A5029413061 @default.
• W3009288510 creator A5031040943 @default.
• W3009288510 creator A5062130571 @default.
• W3009288510 date "2020-10-01" @default.
• W3009288510 modified "2023-10-18" @default.
• W3009288510 title "Caracterización genética de bacterias endofíticas de arroz (Oryza sativa L.) con actividad antimicrobiana contra Burkholderia glumae" @default.
• W3009288510 cites W1705420649 @default.
• W3009288510 cites W1826240101 @default.
• W3009288510 cites W1930726173 @default.
• W3009288510 cites W1930802312 @default.
• W3009288510 cites W1965088110 @default.
• W3009288510 cites W1966237982 @default.
• W3009288510 cites W1970507446 @default.
• W3009288510 cites W1979808915 @default.
• W3009288510 cites W2000837818 @default.
• W3009288510 cites W2011667204 @default.
• W3009288510 cites W2016122690 @default.
• W3009288510 cites W2025651966 @default.
• W3009288510 cites W2054600557 @default.
• W3009288510 cites W2063771504 @default.
• W3009288510 cites W2097910699 @default.
• W3009288510 cites W2111683082 @default.
• W3009288510 cites W2134984740 @default.
• W3009288510 cites W2145118994 @default.
• W3009288510 cites W2154401636 @default.
• W3009288510 cites W2156709176 @default.
• W3009288510 cites W2159620031 @default.
• W3009288510 cites W2166421183 @default.
• W3009288510 cites W2166986886 @default.
• W3009288510 cites W2234551430 @default.
• W3009288510 cites W2293971007 @default.
• W3009288510 cites W2300485800 @default.
• W3009288510 cites W2345140541 @default.
• W3009288510 cites W2346958730 @default.
• W3009288510 cites W2368713186 @default.
• W3009288510 cites W2508198032 @default.
• W3009288510 cites W2563438519 @default.
• W3009288510 cites W2713829388 @default.
• W3009288510 cites W2767159709 @default.
• W3009288510 cites W2772940805 @default.
• W3009288510 cites W2782393269 @default.
• W3009288510 cites W2804363957 @default.
• W3009288510 cites W2896191314 @default.
• W3009288510 cites W2899561228 @default.
• W3009288510 cites W2914322486 @default.
• W3009288510 cites W2914603526 @default.
• W3009288510 cites W2914642505 @default.
• W3009288510 cites W3103855632 @default.
• W3009288510 doi "https://doi.org/10.1016/j.ram.2019.12.002" @default.
• W3009288510 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32147231" @default.
• W3009288510 hasPublicationYear "2020" @default.
• W3009288510 type Work @default.
• W3009288510 sameAs 3009288510 @default.
• W3009288510 citedByCount "3" @default.
• W3009288510 countsByYear W30092885102021 @default.
• W3009288510 countsByYear W30092885102023 @default.
• W3009288510 crossrefType "journal-article" @default.
• W3009288510 hasAuthorship W3009288510A5013941919 @default.
• W3009288510 hasAuthorship W3009288510A5018196885 @default.
• W3009288510 hasAuthorship W3009288510A5029413061 @default.
• W3009288510 hasAuthorship W3009288510A5031040943 @default.
• W3009288510 hasAuthorship W3009288510A5062130571 @default.
• W3009288510 hasBestOaLocation W30092885101 @default.
• W3009288510 hasConcept C153911025 @default.
• W3009288510 hasConcept C86803240 @default.
• W3009288510 hasConceptScore W3009288510C153911025 @default.
• W3009288510 hasConceptScore W3009288510C86803240 @default.
• W3009288510 hasIssue "4" @default.
• W3009288510 hasLocation W30092885101 @default.
• W3009288510 hasOpenAccess W3009288510 @default.
• W3009288510 hasPrimaryLocation W30092885101 @default.
• W3009288510 hasRelatedWork W1624724 @default.
• W3009288510 hasRelatedWork W16941392 @default.
• W3009288510 hasRelatedWork W18673665 @default.
• W3009288510 hasRelatedWork W19607970 @default.
• W3009288510 hasRelatedWork W23858761 @default.
• W3009288510 hasRelatedWork W2755145 @default.
• W3009288510 hasRelatedWork W34031416 @default.
• W3009288510 hasRelatedWork W36157141 @default.
• W3009288510 hasRelatedWork W6680445 @default.
• W3009288510 hasRelatedWork W8530470 @default.
• W3009288510 hasVolume "52" @default.
• W3009288510 isParatext "false" @default.
• W3009288510 isRetracted "false" @default.
• W3009288510 magId "3009288510" @default.
• W3009288510 workType "article" @default.