Matches in SemOpenAlex for { <https://semopenalex.org/work/W3009291205> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W3009291205 endingPage "6" @default.
- W3009291205 startingPage "1" @default.
- W3009291205 abstract "Background: Giardia lamblia is a protozoan parasite, and it is most frequently detected as an intestinal pathogenic parasite in humans worldwide. Objective: To determine the genotype of Giardia lamblia and the sub-assemblages for each genotype using semi-nested PCR and restriction fragment length polymorphism. Methodology: DNA samples were isolated from the stools of 100 patients infected with G. lamblia (after examination by copromicroscopy) and amplified by PCR. The 93 positive samples after using PCR, were digested with NlaIV and RsaI fragment restriction fragment enzymes. The samples were taken randomly and the residence and age for every case were taken. According to the location of residence, the cases were divided into urban areas (the cities) and rural areas (the villages). Result: Genotype A represented (32.3%) {AI (5.3%), AII (26.9%)}, genotype B represented (39.8%) {BIII (22.5%), BIV (17.3%)} and mixed A, B represented (28%). Giardia genotype B was the most common genotype among cases included in this study while sub-genotype AII accounted for the majority of cases. Males were more affected 62 (66.66%) than females 31 (33.33%), (79.6%) cases were from rural areas while only (20.4%) cases were from urban; the highest incidence was between (2-6) years represented 72.1%. The variation in sex, residence and age distribution was found to be statistically significant (PV=0.05, 0.03, 0.03 respectively). Molecular analysis for glutamate dehydrogenase (gdh) gene had been shown to assemble genotypes A and B isolates into four groups: AI, AII, BIII, and BIV by using semi-nested PCR technique. Conclusion: Giardiasis is a still common parasitic infection, particularly in young children in rural areas more than urban areas, and in males more than in females in Sohag Governorate, Upper Egypt. Molecularly it was found that both genotype A (sub-genotypes AI, AII), genotype B (sub-genotypes BIIIm B IV), as well as mixed genotypes A and B, were found in examined patients." @default.
- W3009291205 created "2020-03-13" @default.
- W3009291205 creator A5001565452 @default.
- W3009291205 creator A5044883542 @default.
- W3009291205 creator A5062290534 @default.
- W3009291205 creator A5064275481 @default.
- W3009291205 date "2020-01-01" @default.
- W3009291205 modified "2023-09-26" @default.
- W3009291205 title "Genotyping of Giardia lamblia using semi-nested PCR technique and restriction fragment length polymorphism (RFLP) in Sohag Governorate, Egypt." @default.
- W3009291205 doi "https://doi.org/10.35841/diagnosis-therapy.5.1.1-6" @default.
- W3009291205 hasPublicationYear "2020" @default.
- W3009291205 type Work @default.
- W3009291205 sameAs 3009291205 @default.
- W3009291205 citedByCount "0" @default.
- W3009291205 crossrefType "journal-article" @default.
- W3009291205 hasAuthorship W3009291205A5001565452 @default.
- W3009291205 hasAuthorship W3009291205A5044883542 @default.
- W3009291205 hasAuthorship W3009291205A5062290534 @default.
- W3009291205 hasAuthorship W3009291205A5064275481 @default.
- W3009291205 hasConcept C104317684 @default.
- W3009291205 hasConcept C128319531 @default.
- W3009291205 hasConcept C135763542 @default.
- W3009291205 hasConcept C157431215 @default.
- W3009291205 hasConcept C168007829 @default.
- W3009291205 hasConcept C2777271458 @default.
- W3009291205 hasConcept C2780626423 @default.
- W3009291205 hasConcept C31467283 @default.
- W3009291205 hasConcept C42972112 @default.
- W3009291205 hasConcept C49105822 @default.
- W3009291205 hasConcept C54355233 @default.
- W3009291205 hasConcept C71924100 @default.
- W3009291205 hasConcept C86803240 @default.
- W3009291205 hasConceptScore W3009291205C104317684 @default.
- W3009291205 hasConceptScore W3009291205C128319531 @default.
- W3009291205 hasConceptScore W3009291205C135763542 @default.
- W3009291205 hasConceptScore W3009291205C157431215 @default.
- W3009291205 hasConceptScore W3009291205C168007829 @default.
- W3009291205 hasConceptScore W3009291205C2777271458 @default.
- W3009291205 hasConceptScore W3009291205C2780626423 @default.
- W3009291205 hasConceptScore W3009291205C31467283 @default.
- W3009291205 hasConceptScore W3009291205C42972112 @default.
- W3009291205 hasConceptScore W3009291205C49105822 @default.
- W3009291205 hasConceptScore W3009291205C54355233 @default.
- W3009291205 hasConceptScore W3009291205C71924100 @default.
- W3009291205 hasConceptScore W3009291205C86803240 @default.
- W3009291205 hasIssue "1" @default.
- W3009291205 hasLocation W30092912051 @default.
- W3009291205 hasOpenAccess W3009291205 @default.
- W3009291205 hasPrimaryLocation W30092912051 @default.
- W3009291205 hasRelatedWork W18589908 @default.
- W3009291205 hasRelatedWork W2012853363 @default.
- W3009291205 hasRelatedWork W2023789120 @default.
- W3009291205 hasRelatedWork W2065898617 @default.
- W3009291205 hasRelatedWork W2111110258 @default.
- W3009291205 hasRelatedWork W2114087571 @default.
- W3009291205 hasRelatedWork W2145344135 @default.
- W3009291205 hasRelatedWork W2305742138 @default.
- W3009291205 hasRelatedWork W2389108704 @default.
- W3009291205 hasRelatedWork W2412694326 @default.
- W3009291205 hasRelatedWork W2523291738 @default.
- W3009291205 hasRelatedWork W2808841630 @default.
- W3009291205 hasRelatedWork W2998600574 @default.
- W3009291205 hasRelatedWork W3003898280 @default.
- W3009291205 hasRelatedWork W3006836416 @default.
- W3009291205 hasRelatedWork W3027777953 @default.
- W3009291205 hasRelatedWork W3112155349 @default.
- W3009291205 hasRelatedWork W3129516299 @default.
- W3009291205 hasRelatedWork W3182262469 @default.
- W3009291205 hasRelatedWork W2563100828 @default.
- W3009291205 hasVolume "5" @default.
- W3009291205 isParatext "false" @default.
- W3009291205 isRetracted "false" @default.
- W3009291205 magId "3009291205" @default.
- W3009291205 workType "article" @default.