FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3009530812> ?p ?o ?g. }

• W3009530812 abstract "Abstract Background Persistent infection with hepatitis B virus (HBV) accounts for the majority of hepatocellular carcinoma (HCC), but the molecular mechanisms underlying liver carcinogenesis are still not completely understood. Increasing evidence demonstrates that microRNAs (miRNAs) play significant functional roles in virus–host interactions. The aim of this study was to explore differentially expressed miRNA profiles and investigate the molecular mechanism of miR-0308-3p in HBV-positive HCC carcinogenesis. Methods High-throughput sequencing was used to detect novel miRNAs in three samples of HBV-positive HCC tissue compared to matched HBV-negative HCC tissue. The Cancer Genome Atlas (TCGA) database was used to mine miRNAs related to HBV-positive HCC. Bioinformatics analyses were conducted to predict the miRNAs’ possible biological and pathway regulatory functions. Quantitative polymerase chain reaction (qPCR) was then applied to evaluate the expression levels of randomly selected miRNAs. CCK-8 was used to measure cell proliferation and cell cycles were analyzed using flow cytometry. A dual luciferase reporter gene assay was used to confirm the downstream targets of miR-0308-3p. Results In total, there were 34 overlapping miRNAs in both our miRNA-seq data and the TCGA database. We found two overlapping miRNAs in both the HBV-positive HCC samples and the TCGA database, and 205 novel pre-miRNA sequences were predicted. miR-522 and miR-523 were markedly overexpressed in HBV-positive HCC and were associated with a significantly poorer long-term prognosis (miR-522, HR 2.19, 95% CI 1.33–3.6, p = 0.0015; miR-523HR 1.5, 95% CI 1–2.44, p = 0.0047). Of note, we found that the novel miR-0308-3p was markedly downregulated in HBV-positive HCC samples and HCC cancer cell lines compared with HBV-negative HCC samples and adjacent normal hepatic tissue. Moreover, elevated expression of miR-0308-3p was found to inhibit proliferation of cancer cells by promoting G1/S cell cycle arrest but did not influence the apoptosis of cancer cells. A dual luciferase reporter activity assay identified that miR-0308-3p acted directly on the target sequence of the CDK6 and Cyclin D1 mRNA 3ʹUTR to suppress CDK6 and Cyclin D1 expression. Conclusions MiR-0308-3p upregulation dramatically suppressed HCC cell proliferation and induced G1/S cell cycle arrest by directly targeting CDK6/Cyclin D1. These findings reveal a novel molecular mechanism for activation of G1/S arrest in HCC and may prove clinically useful for developing new therapeutic targets." @default.
• W3009530812 created "2020-03-13" @default.
• W3009530812 creator A5005241892 @default.
• W3009530812 creator A5018984110 @default.
• W3009530812 creator A5036638655 @default.
• W3009530812 creator A5036780597 @default.
• W3009530812 creator A5051676738 @default.
• W3009530812 creator A5075079013 @default.
• W3009530812 creator A5083588367 @default.
• W3009530812 creator A5089961613 @default.
• W3009530812 date "2020-02-27" @default.
• W3009530812 modified "2023-10-11" @default.
• W3009530812 title "A novel miR-0308-3p revealed by miRNA-seq of HBV-positive hepatocellular carcinoma suppresses cell proliferation and promotes G1/S arrest by targeting double CDK6/Cyclin D1 genes" @default.
• W3009530812 cites W1530007295 @default.
• W3009530812 cites W1710605776 @default.
• W3009530812 cites W1864967712 @default.
• W3009530812 cites W1983142022 @default.
• W3009530812 cites W1991901646 @default.
• W3009530812 cites W2014057686 @default.
• W3009530812 cites W2025183726 @default.
• W3009530812 cites W2030081423 @default.
• W3009530812 cites W2030712838 @default.
• W3009530812 cites W2051126469 @default.
• W3009530812 cites W2079340921 @default.
• W3009530812 cites W2125050407 @default.
• W3009530812 cites W2144597430 @default.
• W3009530812 cites W2162611332 @default.
• W3009530812 cites W2165744153 @default.
• W3009530812 cites W2323320914 @default.
• W3009530812 cites W2341442578 @default.
• W3009530812 cites W2366142655 @default.
• W3009530812 cites W2417229567 @default.
• W3009530812 cites W2490768749 @default.
• W3009530812 cites W2517456784 @default.
• W3009530812 cites W2524871795 @default.
• W3009530812 cites W2549606754 @default.
• W3009530812 cites W2591797433 @default.
• W3009530812 cites W2591948158 @default.
• W3009530812 cites W2617575782 @default.
• W3009530812 cites W2658062003 @default.
• W3009530812 cites W2751440157 @default.
• W3009530812 cites W2766563914 @default.
• W3009530812 cites W2768663336 @default.
• W3009530812 cites W2768704196 @default.
• W3009530812 cites W2771300693 @default.
• W3009530812 cites W2781525129 @default.
• W3009530812 cites W2787142790 @default.
• W3009530812 cites W2787340302 @default.
• W3009530812 cites W2793809918 @default.
• W3009530812 cites W2884284889 @default.
• W3009530812 cites W2884326945 @default.
• W3009530812 cites W2894400238 @default.
• W3009530812 cites W2898256353 @default.
• W3009530812 cites W2899769516 @default.
• W3009530812 cites W2902488470 @default.
• W3009530812 cites W2906105449 @default.
• W3009530812 cites W2907605167 @default.
• W3009530812 cites W2911193256 @default.
• W3009530812 cites W2916529938 @default.
• W3009530812 cites W2937977485 @default.
• W3009530812 cites W2945111049 @default.
• W3009530812 cites W2954138549 @default.
• W3009530812 cites W2955324018 @default.
• W3009530812 cites W2959896606 @default.
• W3009530812 cites W2964324661 @default.
• W3009530812 cites W2966550193 @default.
• W3009530812 cites W2966684014 @default.
• W3009530812 cites W2985867343 @default.
• W3009530812 cites W2996435285 @default.
• W3009530812 cites W4211105405 @default.
• W3009530812 cites W639723229 @default.
• W3009530812 doi "https://doi.org/10.1186/s13578-020-00382-7" @default.
• W3009530812 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7047384" @default.
• W3009530812 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32128112" @default.
• W3009530812 hasPublicationYear "2020" @default.
• W3009530812 type Work @default.
• W3009530812 sameAs 3009530812 @default.
• W3009530812 citedByCount "14" @default.
• W3009530812 countsByYear W30095308122020 @default.
• W3009530812 countsByYear W30095308122021 @default.
• W3009530812 countsByYear W30095308122022 @default.
• W3009530812 countsByYear W30095308122023 @default.
• W3009530812 crossrefType "journal-article" @default.
• W3009530812 hasAuthorship W3009530812A5005241892 @default.
• W3009530812 hasAuthorship W3009530812A5018984110 @default.
• W3009530812 hasAuthorship W3009530812A5036638655 @default.
• W3009530812 hasAuthorship W3009530812A5036780597 @default.
• W3009530812 hasAuthorship W3009530812A5051676738 @default.
• W3009530812 hasAuthorship W3009530812A5075079013 @default.
• W3009530812 hasAuthorship W3009530812A5083588367 @default.
• W3009530812 hasAuthorship W3009530812A5089961613 @default.
• W3009530812 hasBestOaLocation W30095308121 @default.
• W3009530812 hasConcept C104317684 @default.
• W3009530812 hasConcept C145059251 @default.
• W3009530812 hasConcept C150194340 @default.
• W3009530812 hasConcept C152724338 @default.
• W3009530812 hasConcept C159047783 @default.
• W3009530812 hasConcept C162317418 @default.
• W3009530812 hasConcept C199649820 @default.
• W3009530812 hasConcept C199835354 @default.

• next