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- W3009660117 abstract "A high-throughput fluorescence assay for amidases is demonstrated. It illustrates a new principle in enzyme assays, whereby changes in the pM value during the reaction are recorded (pM=−lg[M], M=free metal ions). An orange fluorescent quinacridone-based ligand 1 is the pM sensor (shown chelated to a reporter metal ion, M2+=Cu2+ or Ni2+) that is used to monitor the release of metal-chelating amino acids by the enzymes acylase I and aminopeptidase." @default.
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- W3009660117 date "2001-05-04" @default.
- W3009660117 modified "2023-10-03" @default.
- W3009660117 title "An Enzyme Assay Using pM" @default.
- W3009660117 doi "https://doi.org/10.1002/1521-3773(20010504)40:9<1771::aid-anie17710>3.3.co;2-d" @default.
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