FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3009944609> ?p ?o ?g. }

• W3009944609 endingPage "356" @default.
• W3009944609 startingPage "356" @default.
• W3009944609 abstract "Kinetoplastids are protozoa containing a range of ubiquitous free_living species–pathogens of invertebrates, vertebrates and even some plants. Some of them are causative agents of canine vector-borne diseases. Their diagnosis is often missing in a gold standard. Here, we proposed a molecular approach for the diagnosis and study of Kinetoplastida. The TaqMan qPCR assays target the following genes: 24Sa LSU of Kinetoplastida, 28S LSU of Leishmania/ Trypanosoma spp., 5.8S rRNA of Trypanosoma spp., 18S SSU of Leishmania spp., kinetoplast minicircle DNA (kDNA) of L. donovani complex and kDNA of L. infantum, were designed, validated for their sensitivity (Se) and specificity (Sp) in silico and in vitro using a panel of known DNAs. They were then used to screen 369 blood samples (358 dogs, 2 equids, 9 monkeys). In addition, new 28S LSU primer sets are presented to use for Kinetoplastida’s identification by PCR/sequencing. All qPCRs showed consistently high analytical sensitivities and reproducibility. They detect approximately 0.01 parasite/ mL blood for the kDNA based- qPCRs and at least a single cell-equivalent of rDNA for the other systems. Based on the sequencing results, after screening, Se and Sp were: 0. 919 and 0.971, 0.853 and 0.979, 1.00 and 0.987, 0.826 and 0.995 for all of Kinetoplastida, Leishmania/ Trypanosoma, Trypanosoma, Leishmania spp. specific qPCRs, respectively. kDNA based qPCRs were more sensitive and specific (Se: 1.00; Sp: 0.997). PCR/sequencing allowed the detection of Kinetoplastids in animal blood samples such as L. infantum, L. guyanensis, T. congolense, T. evansi and Bodo spp. The molecular approach proposed here is useful for epidemiological studies, fundamental research such as screening for new Kinetoplastida species, diagnosis and therapeutic follow-up. In addition, researchers are free to choose the molecular tools adapted to their aims." @default.
• W3009944609 created "2020-03-13" @default.
• W3009944609 creator A5003122561 @default.
• W3009944609 creator A5010669194 @default.
• W3009944609 creator A5039407890 @default.
• W3009944609 creator A5087816407 @default.
• W3009944609 date "2020-03-02" @default.
• W3009944609 modified "2023-10-04" @default.
• W3009944609 title "New Molecular Approach for the Detection of Kinetoplastida Parasites of Medical and Veterinary Interest" @default.
• W3009944609 cites W121761181 @default.
• W3009944609 cites W1605146840 @default.
• W3009944609 cites W1964108923 @default.
• W3009944609 cites W1964869212 @default.
• W3009944609 cites W1970668301 @default.
• W3009944609 cites W1975055427 @default.
• W3009944609 cites W1977440469 @default.
• W3009944609 cites W1994066209 @default.
• W3009944609 cites W1994702061 @default.
• W3009944609 cites W2001958004 @default.
• W3009944609 cites W2024337911 @default.
• W3009944609 cites W2030387285 @default.
• W3009944609 cites W2032019813 @default.
• W3009944609 cites W2036832110 @default.
• W3009944609 cites W2054357066 @default.
• W3009944609 cites W2055145487 @default.
• W3009944609 cites W2058136774 @default.
• W3009944609 cites W2066374577 @default.
• W3009944609 cites W2066860206 @default.
• W3009944609 cites W2073240335 @default.
• W3009944609 cites W2073302338 @default.
• W3009944609 cites W2077237334 @default.
• W3009944609 cites W2088211934 @default.
• W3009944609 cites W2104330137 @default.
• W3009944609 cites W2105680185 @default.
• W3009944609 cites W2111348995 @default.
• W3009944609 cites W2112315489 @default.
• W3009944609 cites W2115511902 @default.
• W3009944609 cites W2120011724 @default.
• W3009944609 cites W2120800541 @default.
• W3009944609 cites W2127957580 @default.
• W3009944609 cites W2128040248 @default.
• W3009944609 cites W2133449112 @default.
• W3009944609 cites W2141453329 @default.
• W3009944609 cites W2146840772 @default.
• W3009944609 cites W2147324529 @default.
• W3009944609 cites W2148299087 @default.
• W3009944609 cites W2154401636 @default.
• W3009944609 cites W2159008215 @default.
• W3009944609 cites W2159539317 @default.
• W3009944609 cites W2161904760 @default.
• W3009944609 cites W2520957530 @default.
• W3009944609 cites W2535085122 @default.
• W3009944609 cites W2584298728 @default.
• W3009944609 cites W2588226033 @default.
• W3009944609 cites W2623137855 @default.
• W3009944609 cites W2750821234 @default.
• W3009944609 cites W2911593555 @default.
• W3009944609 cites W2950837430 @default.
• W3009944609 cites W2962415013 @default.
• W3009944609 cites W2999895891 @default.
• W3009944609 cites W4378951057 @default.
• W3009944609 cites W44454559 @default.
• W3009944609 doi "https://doi.org/10.3390/microorganisms8030356" @default.
• W3009944609 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7143920" @default.
• W3009944609 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32131458" @default.
• W3009944609 hasPublicationYear "2020" @default.
• W3009944609 type Work @default.
• W3009944609 sameAs 3009944609 @default.
• W3009944609 citedByCount "16" @default.
• W3009944609 countsByYear W30099446092020 @default.
• W3009944609 countsByYear W30099446092021 @default.
• W3009944609 countsByYear W30099446092022 @default.
• W3009944609 countsByYear W30099446092023 @default.
• W3009944609 crossrefType "journal-article" @default.
• W3009944609 hasAuthorship W3009944609A5003122561 @default.
• W3009944609 hasAuthorship W3009944609A5010669194 @default.
• W3009944609 hasAuthorship W3009944609A5039407890 @default.
• W3009944609 hasAuthorship W3009944609A5087816407 @default.
• W3009944609 hasBestOaLocation W30099446091 @default.
• W3009944609 hasConcept C104317684 @default.
• W3009944609 hasConcept C136764020 @default.
• W3009944609 hasConcept C143660080 @default.
• W3009944609 hasConcept C153911025 @default.
• W3009944609 hasConcept C159047783 @default.
• W3009944609 hasConcept C190612196 @default.
• W3009944609 hasConcept C203014093 @default.
• W3009944609 hasConcept C2776027055 @default.
• W3009944609 hasConcept C2776555147 @default.
• W3009944609 hasConcept C2778048844 @default.
• W3009944609 hasConcept C2778566189 @default.
• W3009944609 hasConcept C2778689377 @default.
• W3009944609 hasConcept C2779121891 @default.
• W3009944609 hasConcept C2779549974 @default.
• W3009944609 hasConcept C2781092759 @default.
• W3009944609 hasConcept C2781257258 @default.
• W3009944609 hasConcept C3019824283 @default.
• W3009944609 hasConcept C41008148 @default.
• W3009944609 hasConcept C49105822 @default.

• next