FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3010034984> ?p ?o ?g. }

• W3010034984 endingPage "G869" @default.
• W3010034984 startingPage "G854" @default.
• W3010034984 abstract "DRA (downregulated in adenoma, SLC26A3) and NHE3 (Na + /H + exchanger 3, SLC9A3) together mediate intestinal electroneutral NaCl absorption. Both transporters contain PDZ (postsynaptic density 95, disc large, zonula occludens 1) binding motifs and interact with PDZ adaptor proteins regulating their activity and recycling. SNX27 (sorting nexin 27) contains a PDZ domain and is involved in the recycling of cargo proteins including NHE3. The interaction of SNX27 with DRA and its potential role for the activity and recycling of DRA have been evaluated in this study. SNX27 specifically interacts with DRA via its PDZ domain. The knockdown (KD) of SNX27 reduced DRA activity by 50% but was not accompanied by a decrease of DRA surface expression. This indicates that DRA is trafficked to specific functional domains in the plasma membrane in which DRA is particularly active. Consistently, the disruption of lipid raft integrity by methyl-β-cyclodextrin has an inhibitory effect on DRA activity that was strongly reduced after SNX27 KD. In differentiated intestinal Caco2 cells, superresolution microscopy and a novel quantitative axial approach revealed that DRA and SNX27 colocalize in rab5-positive early endosomes at the apical pole. SNX27 regulates the activity of DRA in the apical plasma membrane through binding with its PDZ domain. This interaction occurs in rab5-positive early endosomes at the apical pole of differentiated intestinal Caco2 cells. SNX27 is involved in the direct recycling of DRA to the plasma membrane where it is inserted into lipid rafts facilitating increased activity. NEW & NOTEWORTHY SNX27 has a PDZ domain and is involved in the regulation and recycling of transmembrane proteins. The role of SNX27 on the activity and recycling of the intestinal Cl − /[Formula: see text] exchanger DRA has not yet been studied. This study shows that SNX27 directly interacts with DRA in early endosomes at the apical pole of intestinal Caco2 cells and mediates its direct recycling to facilitate high activity in lipid rafts in the apical plasma membrane." @default.
• W3010034984 created "2020-03-13" @default.
• W3010034984 creator A5008797926 @default.
• W3010034984 creator A5012794413 @default.
• W3010034984 creator A5044763904 @default.
• W3010034984 creator A5051060247 @default.
• W3010034984 creator A5056477509 @default.
• W3010034984 creator A5058300426 @default.
• W3010034984 creator A5062218486 @default.
• W3010034984 date "2020-05-01" @default.
• W3010034984 modified "2023-10-12" @default.
• W3010034984 title "SNX27 regulates DRA activity and mediates its direct recycling by PDZ-interaction in early endosomes at the apical pole of Caco2 cells" @default.
• W3010034984 cites W1543809086 @default.
• W3010034984 cites W1976541921 @default.
• W3010034984 cites W1978224396 @default.
• W3010034984 cites W1988607142 @default.
• W3010034984 cites W1995411796 @default.
• W3010034984 cites W1998544686 @default.
• W3010034984 cites W2013369484 @default.
• W3010034984 cites W2016045507 @default.
• W3010034984 cites W2021318244 @default.
• W3010034984 cites W2026050056 @default.
• W3010034984 cites W2026489455 @default.
• W3010034984 cites W2046133662 @default.
• W3010034984 cites W2050271023 @default.
• W3010034984 cites W2054883747 @default.
• W3010034984 cites W2056957404 @default.
• W3010034984 cites W2063795338 @default.
• W3010034984 cites W2083829293 @default.
• W3010034984 cites W2091349763 @default.
• W3010034984 cites W2092482155 @default.
• W3010034984 cites W2101133471 @default.
• W3010034984 cites W2105471476 @default.
• W3010034984 cites W2106436554 @default.
• W3010034984 cites W2109384903 @default.
• W3010034984 cites W2116002414 @default.
• W3010034984 cites W2117712813 @default.
• W3010034984 cites W2125099638 @default.
• W3010034984 cites W2128684716 @default.
• W3010034984 cites W2130449935 @default.
• W3010034984 cites W2148545066 @default.
• W3010034984 cites W2149039618 @default.
• W3010034984 cites W2150737519 @default.
• W3010034984 cites W2151040238 @default.
• W3010034984 cites W2156374977 @default.
• W3010034984 cites W2157559409 @default.
• W3010034984 cites W2160305024 @default.
• W3010034984 cites W2199817432 @default.
• W3010034984 cites W2257290296 @default.
• W3010034984 cites W2312836697 @default.
• W3010034984 cites W2415298180 @default.
• W3010034984 cites W2466084781 @default.
• W3010034984 cites W2469540674 @default.
• W3010034984 cites W2552634635 @default.
• W3010034984 cites W2737581548 @default.
• W3010034984 cites W2747981361 @default.
• W3010034984 cites W2907283267 @default.
• W3010034984 cites W313357887 @default.
• W3010034984 cites W423768294 @default.
• W3010034984 cites W628797561 @default.
• W3010034984 cites W2518900853 @default.
• W3010034984 doi "https://doi.org/10.1152/ajpgi.00374.2019" @default.
• W3010034984 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32116023" @default.
• W3010034984 hasPublicationYear "2020" @default.
• W3010034984 type Work @default.
• W3010034984 sameAs 3010034984 @default.
• W3010034984 citedByCount "5" @default.
• W3010034984 countsByYear W30100349842021 @default.
• W3010034984 countsByYear W30100349842022 @default.
• W3010034984 countsByYear W30100349842023 @default.
• W3010034984 crossrefType "journal-article" @default.
• W3010034984 hasAuthorship W3010034984A5008797926 @default.
• W3010034984 hasAuthorship W3010034984A5012794413 @default.
• W3010034984 hasAuthorship W3010034984A5044763904 @default.
• W3010034984 hasAuthorship W3010034984A5051060247 @default.
• W3010034984 hasAuthorship W3010034984A5056477509 @default.
• W3010034984 hasAuthorship W3010034984A5058300426 @default.
• W3010034984 hasAuthorship W3010034984A5062218486 @default.
• W3010034984 hasBestOaLocation W30100349841 @default.
• W3010034984 hasConcept C102747710 @default.
• W3010034984 hasConcept C139770010 @default.
• W3010034984 hasConcept C1491633281 @default.
• W3010034984 hasConcept C154428179 @default.
• W3010034984 hasConcept C185592680 @default.
• W3010034984 hasConcept C2778350249 @default.
• W3010034984 hasConcept C55493867 @default.
• W3010034984 hasConcept C62478195 @default.
• W3010034984 hasConcept C79266657 @default.
• W3010034984 hasConcept C79879829 @default.
• W3010034984 hasConcept C86803240 @default.
• W3010034984 hasConcept C95444343 @default.
• W3010034984 hasConceptScore W3010034984C102747710 @default.
• W3010034984 hasConceptScore W3010034984C139770010 @default.
• W3010034984 hasConceptScore W3010034984C1491633281 @default.
• W3010034984 hasConceptScore W3010034984C154428179 @default.
• W3010034984 hasConceptScore W3010034984C185592680 @default.
• W3010034984 hasConceptScore W3010034984C2778350249 @default.
• W3010034984 hasConceptScore W3010034984C55493867 @default.

• next