Matches in SemOpenAlex for { <https://semopenalex.org/work/W3010062017> ?p ?o ?g. }
- W3010062017 endingPage "116784" @default.
- W3010062017 startingPage "116784" @default.
- W3010062017 abstract "The bacterial resistance caused by the irrational usage of antibiotic has become a global public health crisis. Phage therapy is expected to be an alternative to antibiotics for the treatment of drug-resistant infections. However, industrial separation and purification of phage are cumbersome, time-consuming, high cost, or low efficiency. In this study, two-step salting-out extraction of Klebsiella phage was evaluated. In the first step, under the optimal conditions of 10 wt% sodium citrate/30 wt% ethyl acetate, 99 ± 4.86% of phage was recovered in the bottom phase. Meanwhile, most of the cells and proteins could be removed. Subsequently, n-propanol was added into the bottom phase for the second salting-out extraction. Under the optimal conditions, 77 ± 1.3% of the phage can be recovered in the intermediate phase, and the concentration factor can be as high as 475 ± 17.6. Through the two-step salting-out extraction, the purification fold of phage to total proteins, cells and endotoxins was 19.31 ± 1.26, 18.04 ± 1.3 and 5.19 ± 0.49, respectively, as well as a total phage recovery of 77 ± 2.6%, a total concentration factor of 500 ± 24 and no significant reduction in infectivity of phage. This work provides a potential method for industrial phage purification and has a great significance for the future development of phage therapy." @default.
- W3010062017 created "2020-03-13" @default.
- W3010062017 creator A5001421484 @default.
- W3010062017 creator A5037611710 @default.
- W3010062017 creator A5038540335 @default.
- W3010062017 creator A5040485202 @default.
- W3010062017 creator A5042955722 @default.
- W3010062017 date "2020-07-01" @default.
- W3010062017 modified "2023-10-13" @default.
- W3010062017 title "Separation and purification of Klebsiella phage by two-step salting-out extraction" @default.
- W3010062017 cites W1964024159 @default.
- W3010062017 cites W1965743348 @default.
- W3010062017 cites W1968334024 @default.
- W3010062017 cites W1971229729 @default.
- W3010062017 cites W1975004037 @default.
- W3010062017 cites W1978116622 @default.
- W3010062017 cites W1980633337 @default.
- W3010062017 cites W2002195374 @default.
- W3010062017 cites W2003813754 @default.
- W3010062017 cites W2007249153 @default.
- W3010062017 cites W2007705677 @default.
- W3010062017 cites W2021272316 @default.
- W3010062017 cites W2025513201 @default.
- W3010062017 cites W2036210404 @default.
- W3010062017 cites W2038206835 @default.
- W3010062017 cites W2051271674 @default.
- W3010062017 cites W2054970686 @default.
- W3010062017 cites W2057175200 @default.
- W3010062017 cites W2059510043 @default.
- W3010062017 cites W2060807469 @default.
- W3010062017 cites W2066596304 @default.
- W3010062017 cites W2069862023 @default.
- W3010062017 cites W2083424845 @default.
- W3010062017 cites W2106999995 @default.
- W3010062017 cites W2125381415 @default.
- W3010062017 cites W2132911648 @default.
- W3010062017 cites W2146157756 @default.
- W3010062017 cites W2168519960 @default.
- W3010062017 cites W2340376277 @default.
- W3010062017 cites W2490082788 @default.
- W3010062017 cites W2542493528 @default.
- W3010062017 cites W2558354518 @default.
- W3010062017 cites W2727258187 @default.
- W3010062017 cites W2733046286 @default.
- W3010062017 cites W2759570191 @default.
- W3010062017 cites W2766499163 @default.
- W3010062017 cites W2796042626 @default.
- W3010062017 cites W2797064849 @default.
- W3010062017 cites W2803847277 @default.
- W3010062017 cites W2894790287 @default.
- W3010062017 cites W2897254003 @default.
- W3010062017 cites W2915072186 @default.
- W3010062017 cites W2935519167 @default.
- W3010062017 cites W35080799 @default.
- W3010062017 cites W4250164815 @default.
- W3010062017 doi "https://doi.org/10.1016/j.seppur.2020.116784" @default.
- W3010062017 hasPublicationYear "2020" @default.
- W3010062017 type Work @default.
- W3010062017 sameAs 3010062017 @default.
- W3010062017 citedByCount "8" @default.
- W3010062017 countsByYear W30100620172020 @default.
- W3010062017 countsByYear W30100620172021 @default.
- W3010062017 countsByYear W30100620172022 @default.
- W3010062017 countsByYear W30100620172023 @default.
- W3010062017 crossrefType "journal-article" @default.
- W3010062017 hasAuthorship W3010062017A5001421484 @default.
- W3010062017 hasAuthorship W3010062017A5037611710 @default.
- W3010062017 hasAuthorship W3010062017A5038540335 @default.
- W3010062017 hasAuthorship W3010062017A5040485202 @default.
- W3010062017 hasAuthorship W3010062017A5042955722 @default.
- W3010062017 hasConcept C104317684 @default.
- W3010062017 hasConcept C169490005 @default.
- W3010062017 hasConcept C178790620 @default.
- W3010062017 hasConcept C184651966 @default.
- W3010062017 hasConcept C185592680 @default.
- W3010062017 hasConcept C2779116282 @default.
- W3010062017 hasConcept C3018256011 @default.
- W3010062017 hasConcept C43617362 @default.
- W3010062017 hasConcept C4725764 @default.
- W3010062017 hasConcept C547475151 @default.
- W3010062017 hasConcept C55493867 @default.
- W3010062017 hasConcept C86803240 @default.
- W3010062017 hasConcept C89423630 @default.
- W3010062017 hasConceptScore W3010062017C104317684 @default.
- W3010062017 hasConceptScore W3010062017C169490005 @default.
- W3010062017 hasConceptScore W3010062017C178790620 @default.
- W3010062017 hasConceptScore W3010062017C184651966 @default.
- W3010062017 hasConceptScore W3010062017C185592680 @default.
- W3010062017 hasConceptScore W3010062017C2779116282 @default.
- W3010062017 hasConceptScore W3010062017C3018256011 @default.
- W3010062017 hasConceptScore W3010062017C43617362 @default.
- W3010062017 hasConceptScore W3010062017C4725764 @default.
- W3010062017 hasConceptScore W3010062017C547475151 @default.
- W3010062017 hasConceptScore W3010062017C55493867 @default.
- W3010062017 hasConceptScore W3010062017C86803240 @default.
- W3010062017 hasConceptScore W3010062017C89423630 @default.
- W3010062017 hasLocation W30100620171 @default.
- W3010062017 hasOpenAccess W3010062017 @default.