Matches in SemOpenAlex for { <https://semopenalex.org/work/W3010596265> ?p ?o ?g. }
- W3010596265 endingPage "13" @default.
- W3010596265 startingPage "1" @default.
- W3010596265 abstract "The mechanism of enhanced radiosensitivity induced by mitochondrial uncoupling protein UCP2 was investigated in HeLa cells to provide a theoretical basis as a novel target for cervical cancer treatment.HeLa cells were irradiated with 4 Gy X-radiation at 1.0 Gy/min. The expression of UCP2 mRNA and protein was assayed by real-time quantitative polymerase chain reaction and western blotting. UCP2 siRNA and negative control siRNA fragments were constructed and transfected into HeLa cells 24 h after irradiation. The effect of UCP2 silencing and irradiation on HeLa cells was determined by colony formation, CCK-8 cell viability, γH2AX immunofluorescence assay of DNA damage, Annexin V-FITC/PI apoptosis assay, and propidium iodide cell cycle assay. The effects on mitochondrial structure and function were investigated with fluorescent probes including dichlorodihydrofluorescein diacetate (DCFH-DA) assay of reactive oxygen species (ROS), rhodamine 123, and MitoTracker Green assay of mitochondrial structure and function.Irradiation upregulated UCP2 expression, and UCP2 knockdown decreased the survival of irradiated HeLa cells. UCP2 silencing sensitized HeLa cells to irradiation-induced DNA damage and led to increased apoptosis, cell cycle arrest in G2/M, and increased mitochondrial ROS. Increased radiosensitivity was associated with an activation of P53, decreased Bcl-2, Bcl-xl, cyclin B, CDC2, Ku70, and Rad51 expression, and increased Apaf-1, cytochrome c, caspase-3, and caspase-9 expression.UCP2 inhibition augmented the radiosensitivity of cervical cancer cells, and it may be a potential target of radiotherapy of advanced cervical cancer." @default.
- W3010596265 created "2020-03-13" @default.
- W3010596265 creator A5010267807 @default.
- W3010596265 creator A5025163917 @default.
- W3010596265 creator A5026024877 @default.
- W3010596265 creator A5029059260 @default.
- W3010596265 creator A5068951862 @default.
- W3010596265 creator A5069614177 @default.
- W3010596265 creator A5079045511 @default.
- W3010596265 creator A5091265347 @default.
- W3010596265 date "2020-03-04" @default.
- W3010596265 modified "2023-09-24" @default.
- W3010596265 title "Inhibition of Uncoupling Protein 2 Enhances the Radiosensitivity of Cervical Cancer Cells by Promoting the Production of Reactive Oxygen Species" @default.
- W3010596265 cites W1529592271 @default.
- W3010596265 cites W1594060168 @default.
- W3010596265 cites W1594539479 @default.
- W3010596265 cites W1925799544 @default.
- W3010596265 cites W1978336416 @default.
- W3010596265 cites W1982080917 @default.
- W3010596265 cites W1984142219 @default.
- W3010596265 cites W1998564562 @default.
- W3010596265 cites W2018720655 @default.
- W3010596265 cites W2025059314 @default.
- W3010596265 cites W2049903091 @default.
- W3010596265 cites W2052429455 @default.
- W3010596265 cites W2062168232 @default.
- W3010596265 cites W2082629592 @default.
- W3010596265 cites W2112187175 @default.
- W3010596265 cites W2129846704 @default.
- W3010596265 cites W2142334471 @default.
- W3010596265 cites W2154583417 @default.
- W3010596265 cites W2155235045 @default.
- W3010596265 cites W2183106106 @default.
- W3010596265 cites W2202366653 @default.
- W3010596265 cites W2217231788 @default.
- W3010596265 cites W225038342 @default.
- W3010596265 cites W2272380640 @default.
- W3010596265 cites W2274324631 @default.
- W3010596265 cites W2396717324 @default.
- W3010596265 cites W2405823615 @default.
- W3010596265 cites W2592440901 @default.
- W3010596265 cites W2611332446 @default.
- W3010596265 cites W2617303046 @default.
- W3010596265 cites W2728843724 @default.
- W3010596265 cites W2759605842 @default.
- W3010596265 cites W2794350466 @default.
- W3010596265 cites W2802694260 @default.
- W3010596265 cites W2807007486 @default.
- W3010596265 cites W2807334958 @default.
- W3010596265 cites W2891937532 @default.
- W3010596265 cites W2913964857 @default.
- W3010596265 cites W2914568427 @default.
- W3010596265 cites W2944419623 @default.
- W3010596265 cites W3010319071 @default.
- W3010596265 cites W1874984519 @default.
- W3010596265 doi "https://doi.org/10.1155/2020/5135893" @default.
- W3010596265 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7837772" @default.
- W3010596265 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33532034" @default.
- W3010596265 hasPublicationYear "2020" @default.
- W3010596265 type Work @default.
- W3010596265 sameAs 3010596265 @default.
- W3010596265 citedByCount "5" @default.
- W3010596265 countsByYear W30105962652021 @default.
- W3010596265 countsByYear W30105962652022 @default.
- W3010596265 countsByYear W30105962652023 @default.
- W3010596265 crossrefType "journal-article" @default.
- W3010596265 hasAuthorship W3010596265A5010267807 @default.
- W3010596265 hasAuthorship W3010596265A5025163917 @default.
- W3010596265 hasAuthorship W3010596265A5026024877 @default.
- W3010596265 hasAuthorship W3010596265A5029059260 @default.
- W3010596265 hasAuthorship W3010596265A5068951862 @default.
- W3010596265 hasAuthorship W3010596265A5069614177 @default.
- W3010596265 hasAuthorship W3010596265A5079045511 @default.
- W3010596265 hasAuthorship W3010596265A5091265347 @default.
- W3010596265 hasBestOaLocation W30105962651 @default.
- W3010596265 hasConcept C111337013 @default.
- W3010596265 hasConcept C121332964 @default.
- W3010596265 hasConcept C121608353 @default.
- W3010596265 hasConcept C1491633281 @default.
- W3010596265 hasConcept C153911025 @default.
- W3010596265 hasConcept C185544564 @default.
- W3010596265 hasConcept C185592680 @default.
- W3010596265 hasConcept C190283241 @default.
- W3010596265 hasConcept C206684579 @default.
- W3010596265 hasConcept C2775934118 @default.
- W3010596265 hasConcept C2777366897 @default.
- W3010596265 hasConcept C29537977 @default.
- W3010596265 hasConcept C31573885 @default.
- W3010596265 hasConcept C48349386 @default.
- W3010596265 hasConcept C53227056 @default.
- W3010596265 hasConcept C54355233 @default.
- W3010596265 hasConcept C55493867 @default.
- W3010596265 hasConcept C86803240 @default.
- W3010596265 hasConcept C95444343 @default.
- W3010596265 hasConcept C96232424 @default.
- W3010596265 hasConceptScore W3010596265C111337013 @default.
- W3010596265 hasConceptScore W3010596265C121332964 @default.
- W3010596265 hasConceptScore W3010596265C121608353 @default.