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- W30108092 abstract "Research Article1 April 1994free access The basis for germline specificity of the hobo transposable element in Drosophila melanogaster. B.R. Calvi B.R. Calvi Department of Cellular and Developmental Biology, Harvard University, Cambridge, MA 02138. Search for more papers by this author W.M. Gelbart W.M. Gelbart Department of Cellular and Developmental Biology, Harvard University, Cambridge, MA 02138. Search for more papers by this author B.R. Calvi B.R. Calvi Department of Cellular and Developmental Biology, Harvard University, Cambridge, MA 02138. Search for more papers by this author W.M. Gelbart W.M. Gelbart Department of Cellular and Developmental Biology, Harvard University, Cambridge, MA 02138. Search for more papers by this author Author Information B.R. Calvi1 and W.M. Gelbart1 1Department of Cellular and Developmental Biology, Harvard University, Cambridge, MA 02138. The EMBO Journal (1994)13:1636-1644https://doi.org/10.1002/j.1460-2075.1994.tb06427.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Previous results suggested that the hobo transposable element is active predominantly in the germline of Drosophila. We investigate germline restriction of hobo transposition by testing in vitro modified elements for their ability to mobilize marked elements in vivo. Although intact hobo elements are germline specific, an hsp70 promoter-hobo transposase fusion is active in the soma. Analysis of the hsp70-promoted transcript does not provide evidence for splicing. Moreover, the hobo promoter confers germline bias to a highly sensitive reporter, delta 2-3 P transposase. These results indicate that hobo transposition is germline specific due to regulation of transposase production at the level of transcription. Thus, although hobo is similar to the P transposable element in organization and tissue specificity, it differs in the underlying mechanism governing germline specific activity. Previous ArticleNext Article Volume 13Issue 71 April 1994In this issue RelatedDetailsLoading ..." @default.
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- W30108092 title "The basis for germline specificity of the hobo transposable element in Drosophila melanogaster." @default.
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