Matches in SemOpenAlex for { <https://semopenalex.org/work/W3010864437> ?p ?o ?g. }
- W3010864437 endingPage "106373" @default.
- W3010864437 startingPage "106373" @default.
- W3010864437 abstract "This study demonstrates the presence of α7 nicotinic acetylcholine receptors (nAChR) in B lymphocyte-derived SP-2/0 cells by means of flow cytometry and immunocytochemistry. According to lectin and sandwich ELISA, the α7 subunits expressed in SP-2/0 cells are more glycosylated compared to those expressed in the brain or normal B lymphocytes and are combined with β2 subunits. At zero and negative pipette potentials, either acetylcholine or α7-specific agonist PNU282987 stimulated the ion channel activity in SP-2/0 cells revealed by single channel patch-clamp recordings. The conductivity was within the range of 19 to 39 pS and reversal potential was between -17 mV and +28 mV, the currents were potentiated by α7-specific positive allosteric modulator PNU120596 and were partially blocked by α7-specific antagonist methyllicaconitine (MLA). However, they were oriented downwards suggesting that the channels mediated the cation outflux rather than influx. As shown by Ca2+ imaging studies, PNU282987 did not stimulate immediate Ca2+ influx into SP-2/0 cells. Instead, Ca2+ influx through Ca-release-activated channels (CRACs) was observed within minutes after either PNU282987 or MLA application. It is concluded that SP-2/0 express α7β2 nAChRs, which mediate the cation outflux under negative pipette potentials applied, possibly, due to depolarized membrane or negative surface charge formed by carbohydrate residues. In addition, α7β2 nAChRs may influence CRACs in ion-independent way." @default.
- W3010864437 created "2020-03-23" @default.
- W3010864437 creator A5028893651 @default.
- W3010864437 creator A5032769245 @default.
- W3010864437 creator A5032830414 @default.
- W3010864437 creator A5056955749 @default.
- W3010864437 creator A5064519240 @default.
- W3010864437 creator A5083819128 @default.
- W3010864437 date "2020-05-01" @default.
- W3010864437 modified "2023-09-26" @default.
- W3010864437 title "Unusual properties of α7 nicotinic acetylcholine receptor ion channels in B lymphocyte-derived SP-2/0 cells" @default.
- W3010864437 cites W1497472945 @default.
- W3010864437 cites W1571140265 @default.
- W3010864437 cites W1587611015 @default.
- W3010864437 cites W1847960242 @default.
- W3010864437 cites W1886320289 @default.
- W3010864437 cites W1969805452 @default.
- W3010864437 cites W1973052095 @default.
- W3010864437 cites W1974953816 @default.
- W3010864437 cites W1977958878 @default.
- W3010864437 cites W1989580086 @default.
- W3010864437 cites W1992858113 @default.
- W3010864437 cites W1996461898 @default.
- W3010864437 cites W1998078961 @default.
- W3010864437 cites W1998719957 @default.
- W3010864437 cites W2003285951 @default.
- W3010864437 cites W2005952760 @default.
- W3010864437 cites W2013074035 @default.
- W3010864437 cites W2017542169 @default.
- W3010864437 cites W2019025856 @default.
- W3010864437 cites W2019057590 @default.
- W3010864437 cites W2019269080 @default.
- W3010864437 cites W2020042478 @default.
- W3010864437 cites W2021096057 @default.
- W3010864437 cites W2023499236 @default.
- W3010864437 cites W2026227988 @default.
- W3010864437 cites W2031916288 @default.
- W3010864437 cites W2035582406 @default.
- W3010864437 cites W2037897034 @default.
- W3010864437 cites W2042678786 @default.
- W3010864437 cites W2045369887 @default.
- W3010864437 cites W2049442238 @default.
- W3010864437 cites W2050279099 @default.
- W3010864437 cites W2051306849 @default.
- W3010864437 cites W2054366648 @default.
- W3010864437 cites W2055865789 @default.
- W3010864437 cites W2059234084 @default.
- W3010864437 cites W2061743609 @default.
- W3010864437 cites W20778914 @default.
- W3010864437 cites W2079307058 @default.
- W3010864437 cites W2082705025 @default.
- W3010864437 cites W2085657703 @default.
- W3010864437 cites W2099587101 @default.
- W3010864437 cites W2106341602 @default.
- W3010864437 cites W2114838789 @default.
- W3010864437 cites W2115155277 @default.
- W3010864437 cites W2118958190 @default.
- W3010864437 cites W2124366608 @default.
- W3010864437 cites W2128845098 @default.
- W3010864437 cites W2132781656 @default.
- W3010864437 cites W2141578443 @default.
- W3010864437 cites W2141829216 @default.
- W3010864437 cites W2146710322 @default.
- W3010864437 cites W2154748059 @default.
- W3010864437 cites W2156595997 @default.
- W3010864437 cites W2161605623 @default.
- W3010864437 cites W2169117352 @default.
- W3010864437 cites W2525429856 @default.
- W3010864437 cites W2562638273 @default.
- W3010864437 cites W2731807203 @default.
- W3010864437 cites W2802632767 @default.
- W3010864437 doi "https://doi.org/10.1016/j.intimp.2020.106373" @default.
- W3010864437 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32163855" @default.
- W3010864437 hasPublicationYear "2020" @default.
- W3010864437 type Work @default.
- W3010864437 sameAs 3010864437 @default.
- W3010864437 citedByCount "3" @default.
- W3010864437 countsByYear W30108644372021 @default.
- W3010864437 countsByYear W30108644372022 @default.
- W3010864437 crossrefType "journal-article" @default.
- W3010864437 hasAuthorship W3010864437A5028893651 @default.
- W3010864437 hasAuthorship W3010864437A5032769245 @default.
- W3010864437 hasAuthorship W3010864437A5032830414 @default.
- W3010864437 hasAuthorship W3010864437A5056955749 @default.
- W3010864437 hasAuthorship W3010864437A5064519240 @default.
- W3010864437 hasAuthorship W3010864437A5083819128 @default.
- W3010864437 hasConcept C12554922 @default.
- W3010864437 hasConcept C134018914 @default.
- W3010864437 hasConcept C147944092 @default.
- W3010864437 hasConcept C170493617 @default.
- W3010864437 hasConcept C181911157 @default.
- W3010864437 hasConcept C185592680 @default.
- W3010864437 hasConcept C188987157 @default.
- W3010864437 hasConcept C2775910092 @default.
- W3010864437 hasConcept C2778071365 @default.
- W3010864437 hasConcept C2779570518 @default.
- W3010864437 hasConcept C2780758401 @default.
- W3010864437 hasConcept C33789571 @default.