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• W3010931358 endingPage "104839" @default.
• W3010931358 startingPage "104839" @default.
• W3010931358 abstract "Biogenic amines (BA) are markers of food freshness and indicators of inadequate food processing and storage conditions. Because BA determination is important for food quality evaluation, and considering the relatively complex analytical procedures available, new approaches are required for their determination. In this work, an environmentally friendly analytical procedure is proposed for BA determination in cheese, involving separation by sequential injection chromatography without organic solvents or chemical derivatization of the analytes. Sample preparation step was carried out by acid protein precipitation and low-temperature partition with a reduced volume of organic solvent (500 µL of acetonitrile). Because of the previously mentioned characteristics, an analytical eco-scale = 91 was achieved. Histamine, tyramine, and tryptamine were separated in a cyanopropyl monolithic column in the isocratic elution mode, with 3.0 mmol L−1 phosphoric acid aqueous solution as mobile phase. The analytes were quantified from their intrinsic absorption at 210 nm. The analysis time was less than 3 min, with good chromatographic efficiency (N ≥ 75000 plates m−1). For the three analytes, LOD within 0.05–0.10 mg kg−1, LOQ within 0.14–0.30 mg kg−1, and linear response ranges up to 10 mg kg−1 (histamine and tyramine) and 15 mg kg−1 (tryptamine) were achieved with good precision (CV = 3.0%, n = 12). The proposed procedure was successfully applied to the determination of tyramine in cheese samples, with recoveries in the 81–128% range." @default.
• W3010931358 created "2020-03-23" @default.
• W3010931358 creator A5029387682 @default.
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• W3010931358 date "2020-07-01" @default.
• W3010931358 modified "2023-10-18" @default.
• W3010931358 title "Solventless separation of underivatized biogenic amines by sequential injection chromatography" @default.
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• W3010931358 doi "https://doi.org/10.1016/j.microc.2020.104839" @default.
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