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- W3011320521 abstract "Terpene volatiles are found in many important fruit crops, but their relationship to flavor is poorly understood. Here, we demonstrate using sensory descriptive and discriminant analysis that 1,8-cineole contributes a key floral/eucalyptus note to the aroma of ripe 'Hort16A’ kiwifruit (Actinidia chinensis). Two quantitative trait loci (QTLs) for 1,8-cineole production were identified on linkage groups 27 and 29a in a segregating A. chinensis population, with the QTL on LG29a colocating with a complex cluster of putative terpene synthase (TPS)-encoding genes. Transient expression in Nicotiana benthamiana and analysis of recombinant proteins expressed in Escherichia coli showed four genes in the cluster (AcTPS1a–AcTPS1d) encoded functional TPS enzymes, which produced predominantly sabinene, 1,8-cineole, geraniol, and springene, respectively. The terpene profile produced by AcTPS1b closely resembled the terpenes detected in red-fleshed A. chinensis. AcTPS1b expression correlated with 1,8-cineole content in developing/ripening fruit and also showed a positive correlation with 1,8-cineole content in the mapping population, indicating the basis for segregation is an expression QTL. Transient overexpression of AcTPS1b in Actinidia eriantha fruit confirmed this gene produced 1,8-cineole in Actinidia. Structure-function analysis showed AcTPS1a and AcTPS1b are natural variants at key TPS catalytic site residues previously shown to change enzyme specificity in vitro. Together, our results indicate that AcTPS1b is a key gene for production of the signature flavor terpene 1,8-cineole in ripe kiwifruit. Using a sensory-directed strategy for compound identification provides a rational approach for applying marker-aided selection to improving flavor in kiwifruit as well as other fruits." @default.
- W3011320521 created "2020-03-23" @default.
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- W3011320521 date "2020-03-17" @default.
- W3011320521 modified "2023-10-06" @default.
- W3011320521 title "Sensory-Directed Genetic and Biochemical Characterization of Volatile Terpene Production in Kiwifruit" @default.
- W3011320521 cites W1496968142 @default.
- W3011320521 cites W1521163801 @default.
- W3011320521 cites W1585764830 @default.
- W3011320521 cites W1836198198 @default.
- W3011320521 cites W1856120119 @default.
- W3011320521 cites W1871402226 @default.
- W3011320521 cites W1964032075 @default.
- W3011320521 cites W1968784233 @default.
- W3011320521 cites W1974833847 @default.
- W3011320521 cites W1978794807 @default.
- W3011320521 cites W1980346224 @default.
- W3011320521 cites W1992665072 @default.
- W3011320521 cites W1998086303 @default.
- W3011320521 cites W1998890447 @default.
- W3011320521 cites W2009002262 @default.
- W3011320521 cites W2012197405 @default.
- W3011320521 cites W2022205995 @default.
- W3011320521 cites W2025601876 @default.
- W3011320521 cites W2027269000 @default.
- W3011320521 cites W2027643932 @default.
- W3011320521 cites W2032388739 @default.
- W3011320521 cites W2034501578 @default.
- W3011320521 cites W2036016254 @default.
- W3011320521 cites W2056189298 @default.
- W3011320521 cites W2056258012 @default.
- W3011320521 cites W2057781022 @default.
- W3011320521 cites W2058255651 @default.
- W3011320521 cites W2065947504 @default.
- W3011320521 cites W2070154410 @default.
- W3011320521 cites W2080087312 @default.
- W3011320521 cites W2081184252 @default.
- W3011320521 cites W2088510619 @default.
- W3011320521 cites W2091344286 @default.
- W3011320521 cites W2095335004 @default.
- W3011320521 cites W2097706568 @default.
- W3011320521 cites W2099267861 @default.
- W3011320521 cites W2101509576 @default.
- W3011320521 cites W2104860833 @default.
- W3011320521 cites W2106121393 @default.
- W3011320521 cites W2106246537 @default.
- W3011320521 cites W2116053845 @default.
- W3011320521 cites W2117087701 @default.
- W3011320521 cites W2123695536 @default.
- W3011320521 cites W2125360702 @default.
- W3011320521 cites W2125838338 @default.
- W3011320521 cites W2126074854 @default.
- W3011320521 cites W2126119322 @default.
- W3011320521 cites W2128137074 @default.
- W3011320521 cites W2136037535 @default.
- W3011320521 cites W2141405875 @default.
- W3011320521 cites W2152207030 @default.
- W3011320521 cites W2152458080 @default.
- W3011320521 cites W2153650805 @default.
- W3011320521 cites W2154913580 @default.
- W3011320521 cites W2155016987 @default.
- W3011320521 cites W2159148582 @default.
- W3011320521 cites W2159474015 @default.
- W3011320521 cites W2160818446 @default.
- W3011320521 cites W2163448850 @default.
- W3011320521 cites W2165853297 @default.
- W3011320521 cites W2168920342 @default.
- W3011320521 cites W2170278083 @default.
- W3011320521 cites W2170666070 @default.
- W3011320521 cites W2249219263 @default.
- W3011320521 cites W2416243938 @default.
- W3011320521 cites W2472470407 @default.
- W3011320521 cites W2518072219 @default.
- W3011320521 cites W2530402980 @default.
- W3011320521 cites W2562897222 @default.
- W3011320521 cites W2748632207 @default.
- W3011320521 cites W2774178591 @default.
- W3011320521 cites W2794148246 @default.
- W3011320521 cites W2806038273 @default.
- W3011320521 cites W2883755802 @default.
- W3011320521 cites W2898655184 @default.
- W3011320521 cites W2953222385 @default.
- W3011320521 cites W2956052111 @default.
- W3011320521 cites W3168775563 @default.
- W3011320521 doi "https://doi.org/10.1104/pp.20.00186" @default.
- W3011320521 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7210626" @default.
- W3011320521 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32184346" @default.