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• W3011572331 abstract "Abstract Oligomeric intermediates are implicated as neurotoxins in the pathogenesis of protein mis-folding diseases. Structural, biophysical and biochemical characterisation of these species is challenging due to their heterogeneous and transient nature, and their typically low abun-dance. Here, we show that microfluidic free-flow electrophoresis is capable of separating heterogeneous oligomer mixtures on a timescale of seconds, at least two orders of magnitude faster than conventional techniques. This enables analysis of oligomer structural heterogeneity, zeta-potential and immunochemistry with minimal sample perturbation under physiologically-relevant conditions." @default.
• W3011572331 created "2020-03-23" @default.
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• W3011572331 date "2020-03-14" @default.
• W3011572331 modified "2023-10-16" @default.
• W3011572331 title "Rapid Fractionation and Characterisation of Alpha-Synuclein Oligomers in Solution" @default.
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• W3011572331 doi "https://doi.org/10.1101/2020.03.10.985804" @default.
• W3011572331 hasPublicationYear "2020" @default.
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