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- W3011672446 abstract "Plants surviving under stressed conditions are believed to benefit from associated microorganisms named plant growth promoting bacteria (PGPB). Enterobacter sp. SA187 is a model PGPB that was isolated from the root nodules of Indigofera argentea, Jazan region, Kingdom of Saudi Arabia. SA187 showed several PGPB traits for nutrient acquisition, hormone production, as well as enhancing the growth of crop plants under different abiotic stresses. A complete genome sequence of the SA187 showed different metabolic capacity with 4,347 predicted protein-coding DNA sequences (CDS). To identify the essential genes/ pathways involved in the beneficial SA187/ plant interaction and to correlate different phenotypes and functions to their genomic sequences and pathways, we constructed a saturated mutant library using Tn5 random mutagenesis. The generated library theoretically covered all the genome of SA187 with approximately 16,000 generated clones, all obtained mutants acquired the resistance to the antibiotic Kanamycin as a marker. The Tn5 insertion SA187 library was mapped using Sanger sequencing with specific primers designed for amplification of the flanking region of transposon sequence. Large number of genes were identified including genes involved in mobility, carbohydrates metabolism, surface appendages as well as protein with unknown function, but most importantly, the TrpCF mutant responsible for tryptophan biosynthesis, which eventually contributes to the synthesis of indole3-acetic acid (IAA). Overall, the generated mutagenesis library provides a toolbox to screen and identify the essential genes involved in the SA187 lifestyle as a free-living soil bacterium and as an endophytic bacterium within its host plant." @default.
- W3011672446 created "2020-03-23" @default.
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- W3011672446 date "2019-07-31" @default.
- W3011672446 modified "2023-09-24" @default.
- W3011672446 title "Constructing of a comprehensive transposon library of the plant growth promoting bacterium Enterobacter sp. SA187" @default.
- W3011672446 hasPublicationYear "2019" @default.
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