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• W3011744174 abstract "Detection and quantification of low abundant biomarkers within biological fluids is important but remains extremely challenging in biomedical research as well as clinical diagnostics. Here, we designed and synthesized an ultrabright fluorescent nanoconstruct, termed “plasmonic-fluor”, as an “add-on” label to dramatically improve the signal-to-noise ratio of a wide variety of fluorescence bioassays without altering or complicating the conventional assay workflow or read-out devices. Plasmonic-fluor is highly tunable over the entire visible and near infrared spectrum and up to 6500 times brighter than the conventional fluorophores. Plasmonic fluors can be realized with a variety of fluorophores, including Cy3, Cy5, 680LT and 800CW. The novel nanoconstructs can be readily incorporated into a broad range of bioanalytical methods especially, immuno-microarrays to attain more than 1000 times improvement in the limit-of-detection and dynamic range. Plasmonic nanostructures at the LSPR wavelength exhibit large extinction cross-section, which can be up to 5-6 orders of magnitude larger than light absorption of most organic dyes. This unique property allows utilizing plasmonic-fluors as a multimodal bio-label. Indeed, the binding of plasmonic-fluor to the sensing domains resulted in analyte concentration-dependent color spots, which can be directly visualized by the naked eye. Proteome Profiler Kidney Biomarker Arrays (R&D Systems) qualitatively measuring 38 proteins associated with kidney health and disease were used per manufacturer’s instructions to measure biomarkers in patient urine. After determining fluorescent readout by conventional 800 CW fluor (LI-COR Biosciences) using an Odyssey CLx imager, plasmonic fluor tuned to enhance 800CW fluorescence was added and the microarrays rescanned. Additionally, plasmonic fluor enhanced microarrays were photographed by a cell phone camera in ambient light. Both fluorescent and visible images were quantified using Image Studio Lite software. Imaging using the conventional 800CW fluor detected 26 of 38 analytes over a relative fluorescence scale of 0-13. Analytes such as CXCL16, IL-6, IL-10, MCP-1, MMP-9 and TNF-alpha were not detected. However, the same array when reimaged with plasmonic fluor allowed quantification of all 38 analyte proteins over a relative fluorescence scale of 0-5000. Analysis of the cell phone image allowed quantification of all 38 analytes. A good correlation between the fluorescent and visible acquisition modes (R2=0.88) was found. Plasmonic fluor is an enabling technology to immediately improve the sensitivity of existing methodologies such as microarrays in an easy-to-implement and cost-effective manner. There is applicability of this nanoconstruct as a “visible label” in resource-limited settings to alleviate reliance on immobile and expensive fluorescence readout instruments thus enabling clinical outreach to underserved populations world-wide to diagnose acute kidney injury as a point-of-service assay." @default.
• W3011744174 created "2020-03-23" @default.
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• W3011744174 date "2020-03-01" @default.
• W3011744174 modified "2023-09-26" @default.
• W3011744174 title "SUN-044 PLASMONIC FLUOR PROVIDES ULTRASENSITIVE DETECTION OF KIDNEY INJURY BIOMARKERS ON ANTIBODY SANDWICH MICROARRAYS" @default.
• W3011744174 doi "https://doi.org/10.1016/j.ekir.2020.02.567" @default.
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