FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3011800413> ?p ?o ?g. }

• W3011800413 abstract "Objective Injured tubular reabsorption is highlighted as one of the causes of increased albuminuria in the early stage of diabetic nephropathy; however, the underlying mechanism has not been fully elucidated. In this study, we aimed to explore whether reducing inflammation and remodeling the insulin signaling pathway could improve albumin uptake of renal tubules. Methods 8-week-old male db/db mice (n=8), a type 2 diabetic nephropathy model, administered with nuclear factor kappa-B (NF-κB) inhibitor parthenolide (PTN, 1 mg/kg) intraperitoneally every other day for 8 weeks, were as the treatment group. Meanwhile, the age-matched male db/m mice (n=5) and db/db mice (n=8) were treated with saline as the control group and type 2 diabetic nephropathy group. When the mice were sacrificed, blood and urine were collected to examine homeostasis model assessment of insulin resistance (HOMA-IR) and urine albumin creatinine ratio, and kidney samples were used to analyze histopathologic changes with periodic acid-Schiff (PAS) staining, NF-κB p65, phosphorylation of AKT (p-AKT), amnionless and cubilin expressions with immunohistochemistry as well as western blot, and the albumin uptake of renal tubules by using immunofluorescence. In addition, HKC cells were divided into the insulin group treated with insulin alone, the TNF-α group treated with insulin and tumor necrosis factor (TNF-α), and the TNF-α+PTN group exposed to PTN, insulin and TNF-α. The levels of albumin uptake and expression levels of NF-κB p65, p-IRS-1/IRS-1, p-AKT/AKT, amnionless and cubilin in HKC cells were measured. Results Compared with the db/db group, the db/db+PTN group demonstrated decreased levels of HOMA-IR (36.83±14.09 vs. 31.07±28.05) and urine albumin creatinine ratio (190.3±7.3 vs. 143.0±97.6 mg/mmol); however, the differences were not statistically significant (P>0.05). Periodic acid-Schiff staining showed PTN could alleviate the glomerular hypertrophy and reduce the matrix in mesangial areas of db/db mice. The renal expression of NF-κB p65 was increased and p-AKT (s473) decreased in the db/db group compared with the db/m group (P<0.05). PTN significantly reduced the renal expression of NF-κB p65 and ameliorated the decline of p-AKT (s473) compared with the db/db group (P<0.05). Compared with the db/m group, the expression of amnionless and cubilin decreased and albumin uptake in tubules were reduced in the db/db group (P<0.05), and PTN could significantly increase the expression of cubilin (P<0.05), and improve albumin uptake in tubules. Insulin promoted albumin uptake and the expression of amnionless and cubilin in HKC cells (P<0.05). TNF-α stimulated the expression of NF-κB p65, increased p-IRS-1 (s307) and reduced p-AKT (s473) in HKC cells (P<0.05). In the TNF-α+PTN group, the expression of NF-κB p65 declined and p-IRS-1 (s307) and p-AKT (s473) were restored, compared with the TNF-α group (P<0.05). The expression of amnionless and cubilin decreased in the TNF-α group (P<0.05), and PTN could significantly increase the expression of cubilin (P<0.05). Conclusions Inflammation caused damage to insulin signaling, which reduced amnionless-cubilin expression and albumin uptake. PTN could reduce inflammation and remodel the impaired insulin signaling pathway, which promoted the expression of cubilin and albumin uptake. Our study can shed light on the role of inflammation in the reduction of albumin uptake of renal tubules in type 2 diabetic nephropathy." @default.
• W3011800413 created "2020-03-23" @default.
• W3011800413 creator A5023528183 @default.
• W3011800413 creator A5024301463 @default.
• W3011800413 creator A5025708753 @default.
• W3011800413 creator A5056302397 @default.
• W3011800413 creator A5061265927 @default.
• W3011800413 creator A5063431132 @default.
• W3011800413 date "2020-01-01" @default.
• W3011800413 modified "2023-10-14" @default.
• W3011800413 title "<b>NF-κB Inhibitor Parthenolide Promotes Renal Tubules Albumin Uptake in Type 2 Diabetic Nephropathy</b>" @default.
• W3011800413 cites W1976535742 @default.
• W3011800413 cites W1989109588 @default.
• W3011800413 cites W2030970828 @default.
• W3011800413 cites W2085827306 @default.
• W3011800413 cites W2088529331 @default.
• W3011800413 cites W2101760243 @default.
• W3011800413 cites W2103557717 @default.
• W3011800413 cites W2106124956 @default.
• W3011800413 cites W2127006232 @default.
• W3011800413 cites W2131432849 @default.
• W3011800413 cites W2133632236 @default.
• W3011800413 cites W2145536420 @default.
• W3011800413 cites W2152377123 @default.
• W3011800413 cites W2153710142 @default.
• W3011800413 cites W2153979344 @default.
• W3011800413 cites W2154768306 @default.
• W3011800413 cites W2171126403 @default.
• W3011800413 cites W2176164092 @default.
• W3011800413 cites W2222653645 @default.
• W3011800413 cites W2272561987 @default.
• W3011800413 cites W2330359364 @default.
• W3011800413 cites W2465492279 @default.
• W3011800413 cites W2472425605 @default.
• W3011800413 cites W2481995427 @default.
• W3011800413 cites W2512845699 @default.
• W3011800413 cites W2536523837 @default.
• W3011800413 cites W2611419608 @default.
• W3011800413 cites W2615714795 @default.
• W3011800413 cites W2745033466 @default.
• W3011800413 cites W2768405672 @default.
• W3011800413 cites W2769518750 @default.
• W3011800413 cites W2770928390 @default.
• W3011800413 cites W2810264827 @default.
• W3011800413 cites W2886135897 @default.
• W3011800413 cites W2909903726 @default.
• W3011800413 doi "https://doi.org/10.24920/003573" @default.
• W3011800413 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32299536" @default.
• W3011800413 hasPublicationYear "2020" @default.
• W3011800413 type Work @default.
• W3011800413 sameAs 3011800413 @default.
• W3011800413 citedByCount "1" @default.
• W3011800413 countsByYear W30118004132023 @default.
• W3011800413 crossrefType "journal-article" @default.
• W3011800413 hasAuthorship W3011800413A5023528183 @default.
• W3011800413 hasAuthorship W3011800413A5024301463 @default.
• W3011800413 hasAuthorship W3011800413A5025708753 @default.
• W3011800413 hasAuthorship W3011800413A5056302397 @default.
• W3011800413 hasAuthorship W3011800413A5061265927 @default.
• W3011800413 hasAuthorship W3011800413A5063431132 @default.
• W3011800413 hasBestOaLocation W30118004131 @default.
• W3011800413 hasConcept C104317684 @default.
• W3011800413 hasConcept C126322002 @default.
• W3011800413 hasConcept C134018914 @default.
• W3011800413 hasConcept C159641895 @default.
• W3011800413 hasConcept C185592680 @default.
• W3011800413 hasConcept C190283241 @default.
• W3011800413 hasConcept C2776125364 @default.
• W3011800413 hasConcept C2776415932 @default.
• W3011800413 hasConcept C2779922275 @default.
• W3011800413 hasConcept C2780091579 @default.
• W3011800413 hasConcept C2780306776 @default.
• W3011800413 hasConcept C2780321935 @default.
• W3011800413 hasConcept C2781184683 @default.
• W3011800413 hasConcept C55493867 @default.
• W3011800413 hasConcept C555293320 @default.
• W3011800413 hasConcept C62478195 @default.
• W3011800413 hasConcept C71924100 @default.
• W3011800413 hasConcept C75217442 @default.
• W3011800413 hasConceptScore W3011800413C104317684 @default.
• W3011800413 hasConceptScore W3011800413C126322002 @default.
• W3011800413 hasConceptScore W3011800413C134018914 @default.
• W3011800413 hasConceptScore W3011800413C159641895 @default.
• W3011800413 hasConceptScore W3011800413C185592680 @default.
• W3011800413 hasConceptScore W3011800413C190283241 @default.
• W3011800413 hasConceptScore W3011800413C2776125364 @default.
• W3011800413 hasConceptScore W3011800413C2776415932 @default.
• W3011800413 hasConceptScore W3011800413C2779922275 @default.
• W3011800413 hasConceptScore W3011800413C2780091579 @default.
• W3011800413 hasConceptScore W3011800413C2780306776 @default.
• W3011800413 hasConceptScore W3011800413C2780321935 @default.
• W3011800413 hasConceptScore W3011800413C2781184683 @default.
• W3011800413 hasConceptScore W3011800413C55493867 @default.
• W3011800413 hasConceptScore W3011800413C555293320 @default.
• W3011800413 hasConceptScore W3011800413C62478195 @default.
• W3011800413 hasConceptScore W3011800413C71924100 @default.
• W3011800413 hasConceptScore W3011800413C75217442 @default.
• W3011800413 hasLocation W30118004131 @default.
• W3011800413 hasOpenAccess W3011800413 @default.
• W3011800413 hasPrimaryLocation W30118004131 @default.

• next