FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3011878576> ?p ?o ?g. }

• W3011878576 abstract "Abstract Transplantation of spermatogonial stem cells (SSCs) is an alternative reproductive method to achieve conservation and production of elite animals in livestock production. Creating a recipient animal without endogenous germ cells is important for effective SSC transplantation. However, natural mutants with depletion of SSCs are difficult to obtain, and drug ablation of endogenous germ cells is arduous to perform for practical use. In this study, we used mouse models to study the preparation of recipients with congenital germ cell ablation. We knocked out (KO) Ets-variant gene 5 ( Etv5 ) in mice using the CRISPR/Cas9 system. The testicular weight of Etv5 -/- mice was significantly lower than that of wild-type (WT) mice. The germ cell layer of the seminiferous tubules gradually receded with age in Etv5 -/- mice. At 12 weeks of age, the tubules of Etv5 -/- mice lacked germ cells (Sertoli cell-only syndrome), and sperm were completely absent in the epididymis. We subsequently transplanted allogeneic SSCs with enhanced green fluorescent protein (EGFP) into 3-(immature) or 7-week-old (mature) Etv5 -/- mice. Restoration of germ cell layers in the seminiferous tubules and spermatogenesis was observed in all immature testes but not in mature adult testes at 2 months post-transplantation. The presence of heterologous genes Etv5 and EGFP in recipient testicular tissue and epididymal sperm by PCR indicated that sperm originated from the transplanted donor cells. Our study demonstrates that, although Etv5 -/- mice could accommodate and support foreign germ cell transplantation, this process occurs in a quite low efficiency to support a full spermatogenesis of transplanted SSCs. However, using Etv5 -/- mice as a recipient model for SSC transplantation is feasible, and still needs further investigation to establish an optimized transplantation process." @default.
• W3011878576 created "2020-03-23" @default.
• W3011878576 creator A5025861271 @default.
• W3011878576 creator A5026977205 @default.
• W3011878576 creator A5033790200 @default.
• W3011878576 creator A5036453417 @default.
• W3011878576 creator A5066041681 @default.
• W3011878576 creator A5067483347 @default.
• W3011878576 creator A5069333611 @default.
• W3011878576 creator A5090269873 @default.
• W3011878576 date "2020-03-10" @default.
• W3011878576 modified "2023-09-28" @default.
• W3011878576 title "Establishment of Etv5 gene knockout mice as a recipient model for spermatogonial stem cell transplantation" @default.
• W3011878576 cites W1963910681 @default.
• W3011878576 cites W1985195256 @default.
• W3011878576 cites W1990406664 @default.
• W3011878576 cites W1990574061 @default.
• W3011878576 cites W1997948433 @default.
• W3011878576 cites W2009024144 @default.
• W3011878576 cites W2013670358 @default.
• W3011878576 cites W2021971921 @default.
• W3011878576 cites W2064446194 @default.
• W3011878576 cites W2086010658 @default.
• W3011878576 cites W2096252347 @default.
• W3011878576 cites W2099930565 @default.
• W3011878576 cites W2104956810 @default.
• W3011878576 cites W2106124984 @default.
• W3011878576 cites W2117558473 @default.
• W3011878576 cites W2131717226 @default.
• W3011878576 cites W2144166281 @default.
• W3011878576 cites W2149641988 @default.
• W3011878576 cites W2153353888 @default.
• W3011878576 cites W2153416167 @default.
• W3011878576 cites W2158076879 @default.
• W3011878576 cites W2317131060 @default.
• W3011878576 cites W2768930247 @default.
• W3011878576 cites W2794680309 @default.
• W3011878576 cites W2912429831 @default.
• W3011878576 doi "https://doi.org/10.1101/2020.03.10.985408" @default.
• W3011878576 hasPublicationYear "2020" @default.
• W3011878576 type Work @default.
• W3011878576 sameAs 3011878576 @default.
• W3011878576 citedByCount "0" @default.
• W3011878576 crossrefType "posted-content" @default.
• W3011878576 hasAuthorship W3011878576A5025861271 @default.
• W3011878576 hasAuthorship W3011878576A5026977205 @default.
• W3011878576 hasAuthorship W3011878576A5033790200 @default.
• W3011878576 hasAuthorship W3011878576A5036453417 @default.
• W3011878576 hasAuthorship W3011878576A5066041681 @default.
• W3011878576 hasAuthorship W3011878576A5067483347 @default.
• W3011878576 hasAuthorship W3011878576A5069333611 @default.
• W3011878576 hasAuthorship W3011878576A5090269873 @default.
• W3011878576 hasBestOaLocation W30118785761 @default.
• W3011878576 hasConcept C102230213 @default.
• W3011878576 hasConcept C104317684 @default.
• W3011878576 hasConcept C109825262 @default.
• W3011878576 hasConcept C123765429 @default.
• W3011878576 hasConcept C126322002 @default.
• W3011878576 hasConcept C134018914 @default.
• W3011878576 hasConcept C16613235 @default.
• W3011878576 hasConcept C16685009 @default.
• W3011878576 hasConcept C182704531 @default.
• W3011878576 hasConcept C203014093 @default.
• W3011878576 hasConcept C2778442404 @default.
• W3011878576 hasConcept C2779122487 @default.
• W3011878576 hasConcept C2781068068 @default.
• W3011878576 hasConcept C2781087480 @default.
• W3011878576 hasConcept C28328180 @default.
• W3011878576 hasConcept C2911091166 @default.
• W3011878576 hasConcept C54355233 @default.
• W3011878576 hasConcept C71924100 @default.
• W3011878576 hasConcept C77957584 @default.
• W3011878576 hasConcept C86803240 @default.
• W3011878576 hasConcept C95444343 @default.
• W3011878576 hasConceptScore W3011878576C102230213 @default.
• W3011878576 hasConceptScore W3011878576C104317684 @default.
• W3011878576 hasConceptScore W3011878576C109825262 @default.
• W3011878576 hasConceptScore W3011878576C123765429 @default.
• W3011878576 hasConceptScore W3011878576C126322002 @default.
• W3011878576 hasConceptScore W3011878576C134018914 @default.
• W3011878576 hasConceptScore W3011878576C16613235 @default.
• W3011878576 hasConceptScore W3011878576C16685009 @default.
• W3011878576 hasConceptScore W3011878576C182704531 @default.
• W3011878576 hasConceptScore W3011878576C203014093 @default.
• W3011878576 hasConceptScore W3011878576C2778442404 @default.
• W3011878576 hasConceptScore W3011878576C2779122487 @default.
• W3011878576 hasConceptScore W3011878576C2781068068 @default.
• W3011878576 hasConceptScore W3011878576C2781087480 @default.
• W3011878576 hasConceptScore W3011878576C28328180 @default.
• W3011878576 hasConceptScore W3011878576C2911091166 @default.
• W3011878576 hasConceptScore W3011878576C54355233 @default.
• W3011878576 hasConceptScore W3011878576C71924100 @default.
• W3011878576 hasConceptScore W3011878576C77957584 @default.
• W3011878576 hasConceptScore W3011878576C86803240 @default.
• W3011878576 hasConceptScore W3011878576C95444343 @default.
• W3011878576 hasLocation W30118785761 @default.
• W3011878576 hasLocation W30118785762 @default.
• W3011878576 hasOpenAccess W3011878576 @default.
• W3011878576 hasPrimaryLocation W30118785761 @default.
• W3011878576 hasRelatedWork W12592052 @default.

• next