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• W301198936 abstract "Sleeping Beauty (SB) is a transposon system genetically reconstructed from teleost fish that mediates chromosomal integration of DNA sequences by a cut-and-paste mechanism. SB has been shown to mediate transposition in a variety of cells and tissues, has been used for the generation of transgenic animals and has been tested as a vector for gene therapy in several animal models of human disease. Here, we describe methods that we have developed for testing SB-mediated transposition, first in cultured mammalian cells, and then in vivo using a combination of rapid, high-volume tail vein injection for DNA delivery to the liver along with in vivo bioluminescence imaging to monitor sustained luciferase gene expression in individual animals." @default.
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• W301198936 date "2008-01-01" @default.
• W301198936 modified "2023-10-18" @default.
• W301198936 title "Liver-Directed Gene Therapy Using the Sleeping Beauty Transposon System" @default.
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• W301198936 doi "https://doi.org/10.1007/978-1-60327-248-3_16" @default.
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