FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3012017220> ?p ?o ?g. }

• W3012017220 abstract "Abstract Protein overexpression sometimes causes cellular defects, although the underlying mechanism is still unknown. A protein’s expression limit, which triggers cellular defects, is a useful indication of the underlying mechanism. In this study, we developed an experimental method of estimating the expression limits of target proteins in the human embryonic kidney cell line HEK293 by measuring the proteins’ expression levels in cells that survived after the high-copy introduction of plasmid DNA by which the proteins were expressed under a strong cytomegalovirus promoter. The expression limits of nonfluorescent target proteins were indirectly estimated by measuring the levels of green fluorescent protein (GFP) connected to the target proteins with the self-cleaving sequence P2A. The expression limit of a model GFP was ~5.0% of the total protein, and sustained GFP overexpression caused cell death. The expression limits of GFPs with mitochondria-targeting signals and endoplasmic reticulum localization signals were 1.6% and 0.38%, respectively. The expression limits of four proteins involved in vesicular trafficking were far lower compared to a red fluorescent protein. The protein expression limit estimation method developed will be valuable for defining toxic proteins and consequences of protein overexpression." @default.
• W3012017220 created "2020-03-23" @default.
• W3012017220 creator A5006207706 @default.
• W3012017220 creator A5025670880 @default.
• W3012017220 creator A5042991989 @default.
• W3012017220 creator A5051545544 @default.
• W3012017220 date "2020-03-16" @default.
• W3012017220 modified "2023-10-17" @default.
• W3012017220 title "Development of an experimental method of systematically estimating protein expression limits in HEK293 cells" @default.
• W3012017220 cites W1530368384 @default.
• W3012017220 cites W1594451352 @default.
• W3012017220 cites W1598802541 @default.
• W3012017220 cites W1969489179 @default.
• W3012017220 cites W1996913154 @default.
• W3012017220 cites W2021420018 @default.
• W3012017220 cites W2023545713 @default.
• W3012017220 cites W2034271226 @default.
• W3012017220 cites W2040439697 @default.
• W3012017220 cites W2061104342 @default.
• W3012017220 cites W2066432628 @default.
• W3012017220 cites W2070218019 @default.
• W3012017220 cites W2077060302 @default.
• W3012017220 cites W2080419619 @default.
• W3012017220 cites W2088097090 @default.
• W3012017220 cites W2088180120 @default.
• W3012017220 cites W2097866765 @default.
• W3012017220 cites W2099042745 @default.
• W3012017220 cites W2100122328 @default.
• W3012017220 cites W2116960784 @default.
• W3012017220 cites W2142145420 @default.
• W3012017220 cites W2166357485 @default.
• W3012017220 cites W2219121760 @default.
• W3012017220 cites W2233644193 @default.
• W3012017220 cites W2234388732 @default.
• W3012017220 cites W2341577869 @default.
• W3012017220 cites W2508698766 @default.
• W3012017220 cites W2514377612 @default.
• W3012017220 cites W2593007003 @default.
• W3012017220 cites W2615853094 @default.
• W3012017220 cites W2766900039 @default.
• W3012017220 cites W2887445691 @default.
• W3012017220 cites W2904698639 @default.
• W3012017220 cites W2965272248 @default.
• W3012017220 cites W2965286186 @default.
• W3012017220 cites W374143785 @default.
• W3012017220 cites W805509078 @default.
• W3012017220 doi "https://doi.org/10.1038/s41598-020-61646-3" @default.
• W3012017220 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7075890" @default.
• W3012017220 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32179769" @default.
• W3012017220 hasPublicationYear "2020" @default.
• W3012017220 type Work @default.
• W3012017220 sameAs 3012017220 @default.
• W3012017220 citedByCount "17" @default.
• W3012017220 countsByYear W30120172202020 @default.
• W3012017220 countsByYear W30120172202021 @default.
• W3012017220 countsByYear W30120172202022 @default.
• W3012017220 countsByYear W30120172202023 @default.
• W3012017220 crossrefType "journal-article" @default.
• W3012017220 hasAuthorship W3012017220A5006207706 @default.
• W3012017220 hasAuthorship W3012017220A5025670880 @default.
• W3012017220 hasAuthorship W3012017220A5042991989 @default.
• W3012017220 hasAuthorship W3012017220A5051545544 @default.
• W3012017220 hasBestOaLocation W30120172201 @default.
• W3012017220 hasConcept C104317684 @default.
• W3012017220 hasConcept C142613039 @default.
• W3012017220 hasConcept C153911025 @default.
• W3012017220 hasConcept C158617107 @default.
• W3012017220 hasConcept C174510640 @default.
• W3012017220 hasConcept C33161422 @default.
• W3012017220 hasConcept C40767141 @default.
• W3012017220 hasConcept C54009773 @default.
• W3012017220 hasConcept C54355233 @default.
• W3012017220 hasConcept C55493867 @default.
• W3012017220 hasConcept C6856738 @default.
• W3012017220 hasConcept C81885089 @default.
• W3012017220 hasConcept C86803240 @default.
• W3012017220 hasConcept C95444343 @default.
• W3012017220 hasConceptScore W3012017220C104317684 @default.
• W3012017220 hasConceptScore W3012017220C142613039 @default.
• W3012017220 hasConceptScore W3012017220C153911025 @default.
• W3012017220 hasConceptScore W3012017220C158617107 @default.
• W3012017220 hasConceptScore W3012017220C174510640 @default.
• W3012017220 hasConceptScore W3012017220C33161422 @default.
• W3012017220 hasConceptScore W3012017220C40767141 @default.
• W3012017220 hasConceptScore W3012017220C54009773 @default.
• W3012017220 hasConceptScore W3012017220C54355233 @default.
• W3012017220 hasConceptScore W3012017220C55493867 @default.
• W3012017220 hasConceptScore W3012017220C6856738 @default.
• W3012017220 hasConceptScore W3012017220C81885089 @default.
• W3012017220 hasConceptScore W3012017220C86803240 @default.
• W3012017220 hasConceptScore W3012017220C95444343 @default.
• W3012017220 hasFunder F4320334764 @default.
• W3012017220 hasIssue "1" @default.
• W3012017220 hasLocation W30120172201 @default.
• W3012017220 hasLocation W30120172202 @default.
• W3012017220 hasLocation W30120172203 @default.
• W3012017220 hasLocation W30120172204 @default.
• W3012017220 hasOpenAccess W3012017220 @default.
• W3012017220 hasPrimaryLocation W30120172201 @default.
• W3012017220 hasRelatedWork W1983031293 @default.

• next