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- W3012214096 startingPage "275" @default.
- W3012214096 abstract "Transcription initiation requires formation of the open promoter complex (RPo). To generate RPo, RNA polymerase (RNAP) unwinds the DNA duplex to form the transcription bubble and loads the DNA into the RNAP active site. RPo formation is a multi-step process with transient intermediates of unknown structure. We use single-particle cryoelectron microscopy to visualize seven intermediates containing Escherichia coli RNAP with the transcription factor TraR en route to forming RPo. The structures span the RPo formation pathway from initial recognition of the duplex promoter in a closed complex to the final RPo. The structures and supporting biochemical data define RNAP and promoter DNA conformational changes that delineate steps on the pathway, including previously undetected transient promoter-RNAP interactions that contribute to populating the intermediates but do not occur in RPo. Our work provides a structural basis for understanding RPo formation and its regulation, a major checkpoint in gene expression throughout evolution." @default.
- W3012214096 created "2020-03-23" @default.
- W3012214096 creator A5012675696 @default.
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- W3012214096 creator A5084513610 @default.
- W3012214096 date "2020-04-01" @default.
- W3012214096 modified "2023-10-17" @default.
- W3012214096 title "Stepwise Promoter Melting by Bacterial RNA Polymerase" @default.
- W3012214096 cites W1487777358 @default.
- W3012214096 cites W1505548910 @default.
- W3012214096 cites W1526889047 @default.
- W3012214096 cites W1551727602 @default.
- W3012214096 cites W1590412289 @default.
- W3012214096 cites W1912050508 @default.
- W3012214096 cites W1959539540 @default.
- W3012214096 cites W1972587297 @default.
- W3012214096 cites W1980895652 @default.
- W3012214096 cites W1981577753 @default.
- W3012214096 cites W1984583080 @default.
- W3012214096 cites W1985804692 @default.
- W3012214096 cites W1986191025 @default.
- W3012214096 cites W1993908486 @default.
- W3012214096 cites W1999434982 @default.
- W3012214096 cites W2001792209 @default.
- W3012214096 cites W2004733904 @default.
- W3012214096 cites W2006373999 @default.
- W3012214096 cites W2008687368 @default.
- W3012214096 cites W2009586116 @default.
- W3012214096 cites W2009875303 @default.
- W3012214096 cites W2029582401 @default.
- W3012214096 cites W2029978813 @default.
- W3012214096 cites W2034484189 @default.
- W3012214096 cites W2035503835 @default.
- W3012214096 cites W2036758334 @default.
- W3012214096 cites W2036980961 @default.
- W3012214096 cites W2038061439 @default.
- W3012214096 cites W2041823854 @default.
- W3012214096 cites W2047243608 @default.
- W3012214096 cites W2048326649 @default.
- W3012214096 cites W2052094781 @default.
- W3012214096 cites W2054735322 @default.
- W3012214096 cites W2054901066 @default.
- W3012214096 cites W2063428034 @default.
- W3012214096 cites W2070103798 @default.
- W3012214096 cites W2072021660 @default.
- W3012214096 cites W2072420783 @default.
- W3012214096 cites W2075716499 @default.
- W3012214096 cites W2078906042 @default.
- W3012214096 cites W2081269459 @default.
- W3012214096 cites W2082148398 @default.
- W3012214096 cites W2084622414 @default.
- W3012214096 cites W2086785140 @default.
- W3012214096 cites W2091863327 @default.
- W3012214096 cites W2104234755 @default.
- W3012214096 cites W2104597190 @default.
- W3012214096 cites W2106494572 @default.
- W3012214096 cites W2109364306 @default.
- W3012214096 cites W2111441093 @default.
- W3012214096 cites W2111791895 @default.
- W3012214096 cites W2113904137 @default.
- W3012214096 cites W2125739542 @default.
- W3012214096 cites W2130512803 @default.
- W3012214096 cites W2131884256 @default.
- W3012214096 cites W2132629607 @default.
- W3012214096 cites W2135034797 @default.
- W3012214096 cites W2135296886 @default.
- W3012214096 cites W2140158396 @default.
- W3012214096 cites W2140803417 @default.
- W3012214096 cites W2142161929 @default.
- W3012214096 cites W2143091625 @default.
- W3012214096 cites W2144081223 @default.
- W3012214096 cites W2147057508 @default.
- W3012214096 cites W2148922163 @default.
- W3012214096 cites W2149418298 @default.
- W3012214096 cites W2163748720 @default.
- W3012214096 cites W2171451207 @default.
- W3012214096 cites W2180229411 @default.
- W3012214096 cites W2283441720 @default.
- W3012214096 cites W2299402784 @default.
- W3012214096 cites W2300537338 @default.
- W3012214096 cites W2334348545 @default.
- W3012214096 cites W2358564002 @default.
- W3012214096 cites W2377422330 @default.
- W3012214096 cites W2400779101 @default.
- W3012214096 cites W2552838701 @default.
- W3012214096 cites W2569618521 @default.
- W3012214096 cites W2587625522 @default.
- W3012214096 cites W2593511418 @default.