FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3012465536> ?p ?o ?g. }

• W3012465536 abstract "Abstract Expansion microscopy (ExM) enables super-resolution fluorescence imaging of physically expanded biological samples with conventional microscopes. By combining expansion microscopy (ExM) with single-molecule localization microscopy (SMLM) it is potentially possible to approach the resolution of electron microscopy. However, current attempts to combine both methods remained challenging because of protein and fluorophore loss during digestion or denaturation, gelation, and the incompatibility of expanded polyelectrolyte hydrogels with photoswitching buffers. Here we show that re-embedding of expanded hydrogels enables d STORM imaging of expanded samples and demonstrate that post-labeling ExM resolves the current limitations of super-resolution microscopy. Using microtubules as a reference structure and centrioles, we demonstrate that post-labeling Ex-SMLM preserves ultrastructural details, improves the labeling efficiency and reduces the positional error arising from linking fluorophores into the gel thus paving the way for super-resolution imaging of immunolabeled endogenous proteins with true molecular resolution." @default.
• W3012465536 created "2020-03-23" @default.
• W3012465536 creator A5030507676 @default.
• W3012465536 creator A5032871937 @default.
• W3012465536 creator A5050159969 @default.
• W3012465536 creator A5055107013 @default.
• W3012465536 creator A5066726616 @default.
• W3012465536 creator A5068004563 @default.
• W3012465536 creator A5068607086 @default.
• W3012465536 date "2020-03-13" @default.
• W3012465536 modified "2023-10-14" @default.
• W3012465536 title "Molecular resolution imaging by post-labeling expansion single-molecule localization microscopy (Ex-SMLM)" @default.
• W3012465536 cites W1145147272 @default.
• W3012465536 cites W1785817244 @default.
• W3012465536 cites W1985646440 @default.
• W3012465536 cites W2076115100 @default.
• W3012465536 cites W2079748290 @default.
• W3012465536 cites W2094010512 @default.
• W3012465536 cites W2106316394 @default.
• W3012465536 cites W2115149359 @default.
• W3012465536 cites W2120504118 @default.
• W3012465536 cites W2133059825 @default.
• W3012465536 cites W2133161896 @default.
• W3012465536 cites W2137318843 @default.
• W3012465536 cites W2167279371 @default.
• W3012465536 cites W2296699504 @default.
• W3012465536 cites W2317883772 @default.
• W3012465536 cites W2406725268 @default.
• W3012465536 cites W2460281481 @default.
• W3012465536 cites W2472582738 @default.
• W3012465536 cites W2494923364 @default.
• W3012465536 cites W2593401975 @default.
• W3012465536 cites W2597088123 @default.
• W3012465536 cites W2741475039 @default.
• W3012465536 cites W2742858019 @default.
• W3012465536 cites W2770017816 @default.
• W3012465536 cites W2779346725 @default.
• W3012465536 cites W2789364354 @default.
• W3012465536 cites W2790187745 @default.
• W3012465536 cites W2797351134 @default.
• W3012465536 cites W2887081644 @default.
• W3012465536 cites W2902929870 @default.
• W3012465536 cites W2903629831 @default.
• W3012465536 cites W2905344744 @default.
• W3012465536 cites W2912791793 @default.
• W3012465536 cites W2963774321 @default.
• W3012465536 cites W2969484592 @default.
• W3012465536 cites W2972955994 @default.
• W3012465536 cites W2992498497 @default.
• W3012465536 cites W2998779366 @default.
• W3012465536 cites W4232515155 @default.
• W3012465536 cites W4252713891 @default.
• W3012465536 doi "https://doi.org/10.1101/2020.03.12.988923" @default.
• W3012465536 hasPublicationYear "2020" @default.
• W3012465536 type Work @default.
• W3012465536 sameAs 3012465536 @default.
• W3012465536 citedByCount "3" @default.
• W3012465536 countsByYear W30124655362020 @default.
• W3012465536 countsByYear W30124655362021 @default.
• W3012465536 countsByYear W30124655362022 @default.
• W3012465536 crossrefType "posted-content" @default.
• W3012465536 hasAuthorship W3012465536A5030507676 @default.
• W3012465536 hasAuthorship W3012465536A5032871937 @default.
• W3012465536 hasAuthorship W3012465536A5050159969 @default.
• W3012465536 hasAuthorship W3012465536A5055107013 @default.
• W3012465536 hasAuthorship W3012465536A5066726616 @default.
• W3012465536 hasAuthorship W3012465536A5068004563 @default.
• W3012465536 hasAuthorship W3012465536A5068607086 @default.
• W3012465536 hasBestOaLocation W30124655361 @default.
• W3012465536 hasConcept C105780059 @default.
• W3012465536 hasConcept C115961682 @default.
• W3012465536 hasConcept C120665830 @default.
• W3012465536 hasConcept C121332964 @default.
• W3012465536 hasConcept C12554922 @default.
• W3012465536 hasConcept C138268822 @default.
• W3012465536 hasConcept C141239990 @default.
• W3012465536 hasConcept C147080431 @default.
• W3012465536 hasConcept C154945302 @default.
• W3012465536 hasConcept C166936260 @default.
• W3012465536 hasConcept C169274487 @default.
• W3012465536 hasConcept C185592680 @default.
• W3012465536 hasConcept C187921700 @default.
• W3012465536 hasConcept C192562407 @default.
• W3012465536 hasConcept C2778617687 @default.
• W3012465536 hasConcept C41008148 @default.
• W3012465536 hasConcept C86803240 @default.
• W3012465536 hasConcept C91881484 @default.
• W3012465536 hasConcept C93877712 @default.
• W3012465536 hasConceptScore W3012465536C105780059 @default.
• W3012465536 hasConceptScore W3012465536C115961682 @default.
• W3012465536 hasConceptScore W3012465536C120665830 @default.
• W3012465536 hasConceptScore W3012465536C121332964 @default.
• W3012465536 hasConceptScore W3012465536C12554922 @default.
• W3012465536 hasConceptScore W3012465536C138268822 @default.
• W3012465536 hasConceptScore W3012465536C141239990 @default.
• W3012465536 hasConceptScore W3012465536C147080431 @default.
• W3012465536 hasConceptScore W3012465536C154945302 @default.
• W3012465536 hasConceptScore W3012465536C166936260 @default.
• W3012465536 hasConceptScore W3012465536C169274487 @default.
• W3012465536 hasConceptScore W3012465536C185592680 @default.

• next