FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3012517478> ?p ?o ?g. }

• W3012517478 endingPage "1011" @default.
• W3012517478 startingPage "990" @default.
• W3012517478 abstract "Bioorthogonal chemistry has mainly been developed for proteins and carbohydrates. The chemistry of nucleic acids is different, and bioorthogonal labeling strategies that were successfully applied for proteins and carbohydrates cannot be simply transferred to DNA and RNA. Cycloadditions play a central role for bioorthogonal chemistry with nucleic acids. In vivo postsynthetic labeling of DNA and RNA requires copper-free variants of cycloaddition chemistry to achieve bioorthogonality that can be applied even in living cells. Currently, there are three major types of copper-free cycloadditions available for nucleic acids: (i) the ring-strain-promoted azide-alkyne cycloadditions, (ii) the photoclick 1,3-dipolar cycloadditions, and (iii) the Diels-Alder reactions with inverse electron demand. In principle, bioorthogonally reactive building blocks for postsynthetic modifications of nucleic acids by cycloaddition can be prepared by three different ways: (i) The organic synthesis of DNA and RNA applies phosphoramidites as building blocks for solid-phase automated chemistry. (ii) The biochemical preparation of DNA and RNA by primer extension (PEX) and PCR applies triphosphates as building blocks together with DNA/RNA polymerases, and works in aqueous buffer. (iii) DNA and RNA is labeled by the intrinsic metabolism in cells using bioorthogonally reactive nucleosides. In contrast to proteins and carbohydrates, for which metabolic labeling strategies are well developed, there are only a few examples in the literature for metabolic labeling of nucleic acids. In this review, we summarize the currently available DNA and RNA building blocks, both phosphoramidites and nucleotide triphosphates, for copper-free and bioorthogonal postsynthetic modification strategies." @default.
• W3012517478 created "2020-03-23" @default.
• W3012517478 creator A5002950121 @default.
• W3012517478 creator A5048519602 @default.
• W3012517478 creator A5061000743 @default.
• W3012517478 creator A5064869630 @default.
• W3012517478 creator A5080901916 @default.
• W3012517478 date "2020-03-16" @default.
• W3012517478 modified "2023-09-29" @default.
• W3012517478 title "Postsynthetic Modifications of DNA and RNA by Means of Copper-Free Cycloadditions as Bioorthogonal Reactions" @default.
• W3012517478 cites W1434485260 @default.
• W3012517478 cites W1525086170 @default.
• W3012517478 cites W1771126975 @default.
• W3012517478 cites W178501500 @default.
• W3012517478 cites W1907944904 @default.
• W3012517478 cites W1964619043 @default.
• W3012517478 cites W1964938966 @default.
• W3012517478 cites W1966095188 @default.
• W3012517478 cites W1968222033 @default.
• W3012517478 cites W1968222047 @default.
• W3012517478 cites W1968652549 @default.
• W3012517478 cites W1968666518 @default.
• W3012517478 cites W1968845526 @default.
• W3012517478 cites W1969239697 @default.
• W3012517478 cites W1969425409 @default.
• W3012517478 cites W1973664523 @default.
• W3012517478 cites W1974642741 @default.
• W3012517478 cites W1975895926 @default.
• W3012517478 cites W1976004109 @default.
• W3012517478 cites W1976563942 @default.
• W3012517478 cites W1977762520 @default.
• W3012517478 cites W1978249718 @default.
• W3012517478 cites W1982008058 @default.
• W3012517478 cites W1982713805 @default.
• W3012517478 cites W1983577564 @default.
• W3012517478 cites W1987539590 @default.
• W3012517478 cites W1988901523 @default.
• W3012517478 cites W1989293702 @default.
• W3012517478 cites W1993556264 @default.
• W3012517478 cites W1994050955 @default.
• W3012517478 cites W1995754625 @default.
• W3012517478 cites W1995919915 @default.
• W3012517478 cites W1997099073 @default.
• W3012517478 cites W1997924489 @default.
• W3012517478 cites W1998798346 @default.
• W3012517478 cites W2000547662 @default.
• W3012517478 cites W2000998282 @default.
• W3012517478 cites W2001938114 @default.
• W3012517478 cites W2002916661 @default.
• W3012517478 cites W2008388913 @default.
• W3012517478 cites W2008502734 @default.
• W3012517478 cites W2012483466 @default.
• W3012517478 cites W2012733527 @default.
• W3012517478 cites W2013821696 @default.
• W3012517478 cites W2015410955 @default.
• W3012517478 cites W2023438555 @default.
• W3012517478 cites W2024869506 @default.
• W3012517478 cites W2025217769 @default.
• W3012517478 cites W2025244200 @default.
• W3012517478 cites W2025670433 @default.
• W3012517478 cites W2034136071 @default.
• W3012517478 cites W2034403740 @default.
• W3012517478 cites W2037796752 @default.
• W3012517478 cites W2037929932 @default.
• W3012517478 cites W2039547504 @default.
• W3012517478 cites W2039730742 @default.
• W3012517478 cites W2040555400 @default.
• W3012517478 cites W2040884830 @default.
• W3012517478 cites W2041502322 @default.
• W3012517478 cites W2043821378 @default.
• W3012517478 cites W2046297408 @default.
• W3012517478 cites W2047296469 @default.
• W3012517478 cites W2051609383 @default.
• W3012517478 cites W2054364167 @default.
• W3012517478 cites W2054455698 @default.
• W3012517478 cites W2055261952 @default.
• W3012517478 cites W2056240372 @default.
• W3012517478 cites W2056880409 @default.
• W3012517478 cites W2057315451 @default.
• W3012517478 cites W2059253719 @default.
• W3012517478 cites W2063644720 @default.
• W3012517478 cites W2064611125 @default.
• W3012517478 cites W2067184591 @default.
• W3012517478 cites W2072975221 @default.
• W3012517478 cites W2073517737 @default.
• W3012517478 cites W2074928622 @default.
• W3012517478 cites W2076432328 @default.
• W3012517478 cites W2077790287 @default.
• W3012517478 cites W2081338197 @default.
• W3012517478 cites W2081890528 @default.
• W3012517478 cites W2083413121 @default.
• W3012517478 cites W2089617243 @default.
• W3012517478 cites W2093322394 @default.
• W3012517478 cites W2093338997 @default.
• W3012517478 cites W2094502567 @default.
• W3012517478 cites W2096239099 @default.
• W3012517478 cites W2096952782 @default.
• W3012517478 cites W2097953276 @default.

• next