FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3012565014> ?p ?o ?g. }

• W3012565014 abstract "Abstract Background The stripe rust pathogen, Puccinia striiformis f. sp. tritici ( Pst ), threats world wheat production. Resistance to Pst is often overcome by pathogen virulence changes, but the mechanisms of variation are not clearly understood. To determine the role of mutation in Pst virulence changes, in previous studies 30 mutant isolates were developed from a least virulent isolate using ethyl methanesulfonate (EMS) mutagenesis and phenotyped for virulence changes. The progenitor isolate was sequenced, assembled and annotated for establishing a high-quality reference genome. In the present study, the 30 mutant isolates were sequenced and compared to the wide-type isolate to determine the genomic variation and identify candidates for avirulence ( Avr ) genes. Results The sequence reads of the 30 mutant isolates were mapped to the wild-type reference genome to identify genomic changes. After selecting EMS preferred mutations, 264,630 and 118,913 single nucleotide polymorphism (SNP) sites and 89,078 and 72,513 Indels (Insertion/deletion) were detected among the 30 mutant isolates compared to the primary scaffolds and haplotigs of the wild-type isolate, respectively. Deleterious variants including SNPs and Indels occurred in 1866 genes. Genome wide association analysis identified 754 genes associated with avirulence phenotypes. A total of 62 genes were found significantly associated to 16 avirulence genes after selection through six criteria for putative effectors and degree of association, including 48 genes encoding secreted proteins (SPs) and 14 non-SP genes but with high levels of association ( P ≤ 0.001) to avirulence phenotypes. Eight of the SP genes were identified as avirulence-associated effectors with high-confidence as they met five or six criteria used to determine effectors. Conclusions Genome sequence comparison of the mutant isolates with the progenitor isolate unraveled a large number of mutation sites along the genome and identified high-confidence effector genes as candidates for avirulence genes in Pst. Since the avirulence gene candidates were identified from associated SNPs and Indels caused by artificial mutagenesis, these avirulence gene candidates are valuable resources for elucidating the mechanisms of the pathogen pathogenicity, and will be studied to determine their functions in the interactions between the wheat host and the Pst pathogen." @default.
• W3012565014 created "2020-03-27" @default.
• W3012565014 creator A5001754653 @default.
• W3012565014 creator A5015072928 @default.
• W3012565014 creator A5057831967 @default.
• W3012565014 creator A5064943277 @default.
• W3012565014 creator A5075331489 @default.
• W3012565014 date "2020-03-20" @default.
• W3012565014 modified "2023-10-08" @default.
• W3012565014 title "Whole-genome sequencing of Puccinia striiformis f. sp. tritici mutant isolates identifies avirulence gene candidates" @default.
• W3012565014 cites W1900937478 @default.
• W3012565014 cites W1970280863 @default.
• W3012565014 cites W1975779489 @default.
• W3012565014 cites W1979692920 @default.
• W3012565014 cites W1982102508 @default.
• W3012565014 cites W1990717499 @default.
• W3012565014 cites W1991993713 @default.
• W3012565014 cites W1993275641 @default.
• W3012565014 cites W2002920521 @default.
• W3012565014 cites W2007091390 @default.
• W3012565014 cites W2011651180 @default.
• W3012565014 cites W2012221569 @default.
• W3012565014 cites W2014084419 @default.
• W3012565014 cites W2016059715 @default.
• W3012565014 cites W2020280795 @default.
• W3012565014 cites W2026936889 @default.
• W3012565014 cites W2033174994 @default.
• W3012565014 cites W2048074683 @default.
• W3012565014 cites W2054810412 @default.
• W3012565014 cites W2063117061 @default.
• W3012565014 cites W2067327084 @default.
• W3012565014 cites W2072266670 @default.
• W3012565014 cites W2077299969 @default.
• W3012565014 cites W2101000074 @default.
• W3012565014 cites W2103441770 @default.
• W3012565014 cites W2104484780 @default.
• W3012565014 cites W2104657603 @default.
• W3012565014 cites W2106578986 @default.
• W3012565014 cites W2108796629 @default.
• W3012565014 cites W2110035718 @default.
• W3012565014 cites W2114342250 @default.
• W3012565014 cites W2118859524 @default.
• W3012565014 cites W2119511951 @default.
• W3012565014 cites W2120249321 @default.
• W3012565014 cites W2124061299 @default.
• W3012565014 cites W2131271579 @default.
• W3012565014 cites W2131869897 @default.
• W3012565014 cites W2135777982 @default.
• W3012565014 cites W2136510901 @default.
• W3012565014 cites W2139617161 @default.
• W3012565014 cites W2140952049 @default.
• W3012565014 cites W2143186975 @default.
• W3012565014 cites W2146187800 @default.
• W3012565014 cites W2152770371 @default.
• W3012565014 cites W2152798887 @default.
• W3012565014 cites W2153269523 @default.
• W3012565014 cites W2155611044 @default.
• W3012565014 cites W2156189508 @default.
• W3012565014 cites W2156652398 @default.
• W3012565014 cites W2157502333 @default.
• W3012565014 cites W2161620511 @default.
• W3012565014 cites W2162515800 @default.
• W3012565014 cites W2171209136 @default.
• W3012565014 cites W2204687393 @default.
• W3012565014 cites W2215295950 @default.
• W3012565014 cites W2263772370 @default.
• W3012565014 cites W2311689248 @default.
• W3012565014 cites W2342514051 @default.
• W3012565014 cites W2406722049 @default.
• W3012565014 cites W2511783501 @default.
• W3012565014 cites W2533656718 @default.
• W3012565014 cites W2538143681 @default.
• W3012565014 cites W2560154919 @default.
• W3012565014 cites W2583970955 @default.
• W3012565014 cites W2588109190 @default.
• W3012565014 cites W2606882733 @default.
• W3012565014 cites W2607601109 @default.
• W3012565014 cites W2734818422 @default.
• W3012565014 cites W2750921637 @default.
• W3012565014 cites W2751020399 @default.
• W3012565014 cites W2758731014 @default.
• W3012565014 cites W2769169083 @default.
• W3012565014 cites W2775877430 @default.
• W3012565014 cites W2776178524 @default.
• W3012565014 cites W2787339353 @default.
• W3012565014 cites W2804451349 @default.
• W3012565014 cites W2807287534 @default.
• W3012565014 cites W2890545725 @default.
• W3012565014 cites W2909413744 @default.
• W3012565014 cites W2912990441 @default.
• W3012565014 cites W2914940247 @default.
• W3012565014 cites W2918063357 @default.
• W3012565014 cites W2943780131 @default.
• W3012565014 cites W2949819099 @default.
• W3012565014 cites W2973799422 @default.
• W3012565014 cites W374729652 @default.
• W3012565014 doi "https://doi.org/10.1186/s12864-020-6677-y" @default.
• W3012565014 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7085141" @default.
• W3012565014 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32197579" @default.
• W3012565014 hasPublicationYear "2020" @default.

• next