Matches in SemOpenAlex for { <https://semopenalex.org/work/W301263453> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W301263453 abstract "A translating ribosome protects a discrete fragment of ∼30 nt on its mRNA template from nuclease digestion [Steitz, JA, (1969) Nature 224, 957]. Ribosome profiling determines the positions of active ribosomes on cellular mRNA by deep sequencing the ribosome-protected mRNA fragments [Ingolia, NT et al. (2009) Science 324, 218]. Quantitative measurement of abundance in this complex library is obtained by counting the number of times any given sequence is read by deep sequencing. The rate of protein synthesis is measured by examining the ribosome density of each mRNA.Current methods to isolate polysomes and monosomes rely on several hours of ultracentrifugation using either a sucrose gradient or a cushion. We have investigated size-exclusion chromatography (SEC) in disposable spin-columns as an alternative to ultracentrifugation to isolate polysomes and monosomes for ribosome profiling. The size-exclusion method is simpler and rapid, and does not require any special equipment. Using this method we have streamlined the steps to convert ribosome-protected RNA fragments into libraries compatible with sequencing on Illumina® instruments.We present ribosome profiling data generated using these methods in conjunction with bioinformatics tools for analyzing the deep sequencing data in mammalian samples." @default.
- W301263453 created "2016-06-24" @default.
- W301263453 creator A5008116814 @default.
- W301263453 creator A5027243729 @default.
- W301263453 creator A5042043179 @default.
- W301263453 creator A5056872061 @default.
- W301263453 creator A5087409122 @default.
- W301263453 date "2013-05-01" @default.
- W301263453 modified "2023-09-23" @default.
- W301263453 title "Assessing mRNA Translation: Deep Sequencing of Ribosome Footprints" @default.
- W301263453 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3635272" @default.
- W301263453 hasPublicationYear "2013" @default.
- W301263453 type Work @default.
- W301263453 sameAs 301263453 @default.
- W301263453 citedByCount "0" @default.
- W301263453 crossrefType "journal-article" @default.
- W301263453 hasAuthorship W301263453A5008116814 @default.
- W301263453 hasAuthorship W301263453A5027243729 @default.
- W301263453 hasAuthorship W301263453A5042043179 @default.
- W301263453 hasAuthorship W301263453A5056872061 @default.
- W301263453 hasAuthorship W301263453A5087409122 @default.
- W301263453 hasConcept C104317684 @default.
- W301263453 hasConcept C105580179 @default.
- W301263453 hasConcept C132917006 @default.
- W301263453 hasConcept C141231307 @default.
- W301263453 hasConcept C149364088 @default.
- W301263453 hasConcept C182325514 @default.
- W301263453 hasConcept C54689828 @default.
- W301263453 hasConcept C55493867 @default.
- W301263453 hasConcept C65666806 @default.
- W301263453 hasConcept C67705224 @default.
- W301263453 hasConcept C70721500 @default.
- W301263453 hasConcept C86803240 @default.
- W301263453 hasConcept C88478588 @default.
- W301263453 hasConceptScore W301263453C104317684 @default.
- W301263453 hasConceptScore W301263453C105580179 @default.
- W301263453 hasConceptScore W301263453C132917006 @default.
- W301263453 hasConceptScore W301263453C141231307 @default.
- W301263453 hasConceptScore W301263453C149364088 @default.
- W301263453 hasConceptScore W301263453C182325514 @default.
- W301263453 hasConceptScore W301263453C54689828 @default.
- W301263453 hasConceptScore W301263453C55493867 @default.
- W301263453 hasConceptScore W301263453C65666806 @default.
- W301263453 hasConceptScore W301263453C67705224 @default.
- W301263453 hasConceptScore W301263453C70721500 @default.
- W301263453 hasConceptScore W301263453C86803240 @default.
- W301263453 hasConceptScore W301263453C88478588 @default.
- W301263453 hasLocation W3012634531 @default.
- W301263453 hasOpenAccess W301263453 @default.
- W301263453 hasPrimaryLocation W3012634531 @default.
- W301263453 hasRelatedWork W14947108 @default.
- W301263453 hasRelatedWork W2023237059 @default.
- W301263453 hasRelatedWork W2058541698 @default.
- W301263453 hasRelatedWork W2068239108 @default.
- W301263453 hasRelatedWork W2077710278 @default.
- W301263453 hasRelatedWork W2083160212 @default.
- W301263453 hasRelatedWork W2131902806 @default.
- W301263453 hasRelatedWork W2158307983 @default.
- W301263453 hasRelatedWork W2168138973 @default.
- W301263453 hasRelatedWork W2177784250 @default.
- W301263453 hasRelatedWork W2417089824 @default.
- W301263453 hasRelatedWork W2430684772 @default.
- W301263453 hasRelatedWork W2804929933 @default.
- W301263453 hasRelatedWork W2951280166 @default.
- W301263453 hasRelatedWork W2998026663 @default.
- W301263453 hasRelatedWork W3098529469 @default.
- W301263453 hasRelatedWork W3188596023 @default.
- W301263453 hasRelatedWork W3199765866 @default.
- W301263453 hasRelatedWork W67639098 @default.
- W301263453 hasRelatedWork W94679499 @default.
- W301263453 hasVolume "24" @default.
- W301263453 isParatext "false" @default.
- W301263453 isRetracted "false" @default.
- W301263453 magId "301263453" @default.
- W301263453 workType "article" @default.